Altering effects of caffeic acid phenethyl ester (CAPE) and ischemia/reperfusion injury: an experimental study in a rat TRAM flap model

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dc.contributor.authorYegin, Mehmet Emre
dc.contributor.authorBilkay, Ufuk
dc.contributor.authorTiftikciodlu, Yigit Ozer
dc.contributor.authorUyanikgil, Yigit
dc.contributor.authorCavusodlu, Turker
dc.contributor.authorErcan, Gulinnaz
dc.contributor.authorGurdal, Mehmet
dc.date.accessioned2020-12-01T12:01:16Z
dc.date.available2020-12-01T12:01:16Z
dc.date.issued2020
dc.departmentEge Üniversitesien_US
dc.description.abstractBackground Many studies have been performed since the discovery of ischemia-reperfusion injury (I/R) to find substances that reverse its effects on flaps. However, because of minor or major side effects these substances are mostly failed to be put in clinical practice. in this study, the mitigation effects of caffeic acid phenethyl ester (CAPE) were investigated for its protective effects on flap necrosis in a rat model. Methods Forty-two randomized male Sprague-Dawley rats weighing between 250 and 300 g were assigned to the regular flap (G1) (n = 14), ischemic flap (G2) (n = 14), and ischemic flap + CAPE (G3) (n = 14) groups. Bilateral pedicled TRAM flaps were designed for the first seven rats of each group,; bilateral-free TRAM flaps were designed for the remaining rats in each group and referred as subgroup 1 and 2 (S1 and S2), respectively. in G1, flaps were returned to their original locations without clamping or injection. in G2 and G3, a 2-h ischemia was performed. Before the ischemic process, intraperitoneal saline and CAPE solution was administered intraperitoneally in the second and third groups, respectively. on day 7 after the operation, all flaps were harvested and subjected to biochemical (malondialdehyde, superoxide dismutase, and catalase levels) and histological examinations (hematoxylin & eosin, Mallory-Azan, anti-VEGF, anti-BAX, anti Bcl-2, and anti-iNOS stainings), respectively. Enzyme levels and the Verhofstadt scores showed a statistically significant difference in flaps among the groups. Less injury was noted in G3 than in G2 (p < 0.01). Conclusions in this experimental model, CAPE was effective in protecting tissues against ischemia-reperfusion injury. Level of evidence: no ratableen_US
dc.description.sponsorshipEge University Directory, Scientific Research BureauEge Universityen_US
dc.description.sponsorshipThis project was funded by the Ege University Directory, Scientific Research Bureau.en_US
dc.identifier.doi10.1007/s00238-020-01637-9
dc.identifier.endpage534en_US
dc.identifier.issn0930-343X
dc.identifier.issn1435-0130
dc.identifier.issn0930-343Xen_US
dc.identifier.issn1435-0130en_US
dc.identifier.issue5en_US
dc.identifier.scopus2-s2.0-85085500995en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage527en_US
dc.identifier.urihttps://doi.org/10.1007/s00238-020-01637-9
dc.identifier.urihttps://hdl.handle.net/11454/62368
dc.identifier.volume43en_US
dc.identifier.wosWOS:000534210500001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofEuropean Journal of Plastic Surgeryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCaffeic acid phenethyl esteren_US
dc.subjectFlapen_US
dc.subjectIschemia-reperfusion injuryen_US
dc.titleAltering effects of caffeic acid phenethyl ester (CAPE) and ischemia/reperfusion injury: an experimental study in a rat TRAM flap modelen_US
dc.typeArticleen_US

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