Astragaloside IV and cycloastragenol production in in vitro produced Astragalus trojanus Stev.

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dc.contributor.authorNartop P.
dc.contributor.authorGürel A.
dc.contributor.authorAkgün I.H.
dc.contributor.authorBedir E.
dc.date.accessioned2019-10-26T21:21:01Z
dc.date.available2019-10-26T21:21:01Z
dc.date.issued2015
dc.departmentEge Üniversitesien_US
dc.description.abstractIn the present paper, an efficient method for clonal propagation of an endemic plant, Astragalus trojanus Stev., was described. Effect of sucrose and 6-benzyladenine (BA) concentrations on shoot proliferation and rooting was studied. Woody plant medium (WPM) supplemented with two different concentrations of BA (S1 medium with 0.5 mg/L BA&S2 medium with 1 mg/L BA) was used, where S2 medium resulted in higher rates in both shoot elongation and multiple shoot proliferation of clonally propagated A. trojanus shoot cultures. Of 27 clones, 3 were selected for further studies based on the highest regeneration capacity (Clones 1, 12&15). A total of 340 shoots were obtained from Clone 1 in S1 medium. In this medium, the rooting ratio of shoots for Clone 15 was 11.67%. The highest shoot elongation (1.99 cm), multiple shoot proliferation ratio (4.35) and multiple shoot proliferation percentage (91.67%) were obtained from Clone 1 in S2 medium. Two media, WPM medium supplemented with 15 g/L (K1 medium) and 30 g/L (K2 medium) sucrose were used for rooting. The root formation of Clone 1 was 26.67% in K2 medium. The highest astragaloside IV (0.5486 µg/mg) and cycloastragenol (0.1263 µg/mg) contents were detected in roots grown in K1 medium.en_US
dc.identifier.endpage546en_US
dc.identifier.issn0972-5849
dc.identifier.issn0972-5849en_US
dc.identifier.issue4en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage540en_US
dc.identifier.urihttps://hdl.handle.net/11454/16827
dc.identifier.volume14en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherNational Institute of Science Communication and Information Resources (NISCAIR)en_US
dc.relation.ispartofIndian Journal of Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAstragaloside IVen_US
dc.subjectAstragalus trojanusen_US
dc.subjectCycloastragenolen_US
dc.subjectMultiple shoot proliferationen_US
dc.subjectRootingen_US
dc.titleAstragaloside IV and cycloastragenol production in in vitro produced Astragalus trojanus Stev.en_US
dc.typeArticleen_US

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