Primary insect cell culture from total embryo and embryonic brain tissue of Periplaneta americana: A preliminary study

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dc.contributor.authorSoya S.
dc.contributor.authorCan H.
dc.contributor.authorYikilmaz M.S.
dc.date.accessioned2019-10-27T08:21:15Z
dc.date.available2019-10-27T08:21:15Z
dc.date.issued2015
dc.departmentEge Üniversitesien_US
dc.description.abstractThe aim of this preliminary study was to establish a primary insect cell culture from total embryos and embryonic brain tissues of Periplaneta americana, collected from Izmir, Turkey. Cells were cultured at 29°C in Grace's insect medium for one month. In the embryonic brain tissue culture, single cells and cell clumps containing spherical and ovoid as well as dividing cells were observed. Single bipolar neurons were detected after 4 days in culture. Network systems comprised of bipolar neurons were observed on the 5th day of incubation. In addition, presumably glia cells were observed in the embryonic brain culture. In the total embryo culture, the cell population exhibited variable morphologies, including spherical, spindle-like, polygonal and giant cells after nearly 20 days; the culture covered almost half of the Petri dish area within 30 days. This preliminary study associated with Periplaneta americana primary cell culture is the first of its kind in Turkey. These results should contribute to the development of new insect cell lines that are indigenous to Turkey.en_US
dc.identifier.doi10.2298/ABS150302096C
dc.identifier.endpage1208en_US
dc.identifier.issn0354-4664
dc.identifier.issn0354-4664en_US
dc.identifier.issue4en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage1203en_US
dc.identifier.urihttps://doi.org/10.2298/ABS150302096C
dc.identifier.urihttps://hdl.handle.net/11454/25967
dc.identifier.volume67en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherInstitut za Bioloska Istrazivanjaen_US
dc.relation.ispartofArchives of Biological Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBrain tissueen_US
dc.subjectEmbryoen_US
dc.subjectInsect cell cultureen_US
dc.subjectPeriplaneta americanaen_US
dc.titlePrimary insect cell culture from total embryo and embryonic brain tissue of Periplaneta americana: A preliminary studyen_US
dc.typeArticleen_US

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