Effects of salt stress on total RNA and Poly(A)+RNA contents in tobacco suspension cultured cells at the different time course

Suspension-cultured cells derived from pith cells of Nicotiana tabacum L. cv. Xanthi were used in experiments designed to determination of the effects of salt stress on total RNA and Poly(A)+RNA contents at the different time course. Cultured cells were treated with 70 mM NaCl. Cells were harvested 7th day and 12th day of growing phase from control and stress treated media. In comparing stress and control, statistically differences in amount of total RNA and Poly(A)+RNA were found. Also statistically differences were found depending on a time course. Total RNA was found 162.80 µg g-1 of cell fresh weight (CFW) at the 7th day control, 195.00 µg g-1 of CFW at the 7th day salt imposed, 135.40 µg g-1 of CFW at the 12th day control, 181.40 µg g-1 of CFW at the 12th day salt imposed cells as an average of replications. Poly (A)+RNA was found respectively 3.13, 3.90, 2.41, 3.65 µg g-1 of CFW. All these data clearly indicate that the tobacco cells growing in salt stress conditions show distinct changes in the pattern of accumulation of total RNA and Poly(A) +RNA for the synthesis of salt stress-specific proteins at the different time course. © 2006 Asian Network of Scientific Information.