Electrochemical Determination of 6-Thioguanine and Its Interaction with DNA Oligonucleotides Using Disposable Graphite Pencil Electrodes

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dc.contributor.authorUnal, Didem Nur
dc.contributor.authorEksin, Ece
dc.contributor.authorErdem, Arzum
dc.date.accessioned2019-10-27T10:44:21Z
dc.date.available2019-10-27T10:44:21Z
dc.date.issued2018
dc.departmentEge Üniversitesien_US
dc.description.abstractDisposable graphite pencil electrodes were used in this study for the electrochemical determination of 6-thioguanine using its interaction with a single-stranded Oligo (A)(25) composed of 25 adenine (A) bases or double-stranded Oligo (A)(25)-Oligo (T) 25, a hybrid form of Oligo (A)(25) and Oligo (T)(25) composed of 25 thymine (T) bases. The surface interaction was evaluated by monitoring the changes of the oxidation signals of 6-thioguanine and adenine. The electrochemical measurements were performed using differential pulse voltammetry, cyclic voltammetry, and electrochemical impedance spectroscopy. The experimental conditions, including the concentration of 6-thioguanine and its period of interaction with single-stranded Oligo (A)(25) or double-stranded Oligo (A)(25)-Oligo (T)(25), were optimized by observing the changes in the 6-thioguanine and adenine oxidation currents by differential pulse voltammetry. The detection limit was determined to be 0.24 mu M across the useful linear concentration range from 1 to 4 mu M 6-thioguanine. The reported single-use graphite sensor provides a cost-effective, rapid, and sensitive detection approach for the electrochemical characterization of the interaction between DNA and 6-thioguanine.en_US
dc.description.sponsorshipTurkish Academy of Sciences (TUBA)Turkish Academy of Sciencesen_US
dc.description.sponsorshipAE would like to express gratitude to the Turkish Academy of Sciences (TUBA) as a principal member for its partial support.en_US
dc.identifier.doi10.1080/00032719.2017.1338714
dc.identifier.endpage278en_US
dc.identifier.issn0003-2719
dc.identifier.issn1532-236X
dc.identifier.issn0003-2719en_US
dc.identifier.issn1532-236Xen_US
dc.identifier.issue01.Feben_US
dc.identifier.startpage265en_US
dc.identifier.urihttps://doi.org/10.1080/00032719.2017.1338714
dc.identifier.urihttps://hdl.handle.net/11454/30958
dc.identifier.volume51en_US
dc.identifier.wosWOS:000416519900021en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Incen_US
dc.relation.ispartofAnalytical Lettersen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subject6-thioguanineen_US
dc.subjectcyclic voltammetry (CV)en_US
dc.subjectdifferential pulse voltammetry (DPV)en_US
dc.subjectdrug-DNA interactionen_US
dc.subjectelectrochemical impedance spectroscopy (EIS)en_US
dc.titleElectrochemical Determination of 6-Thioguanine and Its Interaction with DNA Oligonucleotides Using Disposable Graphite Pencil Electrodesen_US
dc.typeArticleen_US

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