Magnetic Nanofiber Layers as a Functional Surface for Biomolecule Immobilization and One-Use ‘Sensing in-a-Drop’ Applications

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dc.contributor.authorSanli S.
dc.contributor.authorCelik E.G.
dc.contributor.authorDemir B.
dc.contributor.authorGumus Z.P.
dc.contributor.authorIlktac R.
dc.contributor.authorAksuner N.
dc.contributor.authorDemirkol D.O.
dc.contributor.authorTimur S.
dc.date.accessioned2019-10-27T08:01:34Z
dc.date.available2019-10-27T08:01:34Z
dc.date.issued2018
dc.departmentEge Üniversitesien_US
dc.description.abstractStabilization of biomolecules on matrices is critically important. Here we constructed composite nanofibers with magnetic features by electro-spinning of magnetic nanoparticles (MNP) in an appropriate polymer matrix on a collector surface for multiple uses. ‘Poly(vinylalcohol)-polyethyleneimine/Fe3O4 magnetic nanoparticles’ (PVA-PEI/MNP) composite nanofiber was used on the screen printed electrodes (SPE) to serve as a platform for the glucose oxidase (GOx) biofilm as the model biomolecule. To show the potential application of this material as a biosensor component, the resulted biofilm, called ‘PVA-PEI/MNP/GOx’, was fixed on SPE via a neodymium magnet for the electrochemical detection of glucose at -0.7 V where oxygen consumption due to enzymatic reaction was measured. The main advantage of the obtained magnetic biomembrane is that it allows analysis with a single drop. After step-by-step surface modifications, analytical characterization was performed using various techniques, such as voltammetry, electrochemical impedance spectroscopy, Fourier-transform infrared spectroscopy and X-Ray photoelectron spectroscopy as well as microscopic techniques. The linear range of the PVA-PEI/MNP/GOx surface was found as 0.0125 to 0.5 mM with a limit of detection of 11.5 µM for glucose as analyte. The application of PVA-PEI/MNP/GOx for glucose detection in synthetic samples was carried out. Data proved the potential use of the ‘PVA-PEI/MNP’ surface as a biomolecule immobilization platform for disposable biosensor applications. © 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheimen_US
dc.identifier.doi10.1002/slct.201802602
dc.identifier.endpage13560en_US
dc.identifier.issn2365-6549
dc.identifier.issn2365-6549en_US
dc.identifier.issue48en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage13553en_US
dc.identifier.urihttps://doi.org/10.1002/slct.201802602
dc.identifier.urihttps://hdl.handle.net/11454/25020
dc.identifier.volume3en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherWiley-Blackwellen_US
dc.relation.ispartofChemistrySelecten_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBiosensorsen_US
dc.subjectEnzyme immobilizationen_US
dc.subjectMagnetic nanoparticlesen_US
dc.subjectNanofiberen_US
dc.subjectSurface modificationen_US
dc.titleMagnetic Nanofiber Layers as a Functional Surface for Biomolecule Immobilization and One-Use ‘Sensing in-a-Drop’ Applicationsen_US
dc.typeArticleen_US

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