Determination of acrylamide in three different bread types by an in-house validated LC-MS/MS method
Küçük Resim Yok
Tarih
2015
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Akademiai Kiado Rt.
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
A rapid and reliable liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) method was developed for the determination of acrylamide in three different local bread types; wheat bread, bran bread, whole wheat bread. Acrylamide analyses were made in crust parts of the 85 bread samples. The method was linear up to 750 µg kg-1 food with a determination coeffi cient of 0.999. Recovery rate was found 99.3% with limit of detection and limit of quantifi cation values of 1.5 µg kg-1 and 5.0 µg kg-1, respectively. Certifi ed reference materials of crisp bread were analysed and acrylamide contents of these samples were found in the range cited in the certifi cates. Statistical correlations were investigated between acrylamide contents and protein contents, reducing sugar contents, moisture contents, pH, and colour parameters (L*, a*, b*) of bread samples. Sample preparation procedure and chromatographic conditions of acrylamide analysis were investigated in more detail, and a rapid, accurate, precise, and reliable analysis method was developed. © 2015 Akadémiai Kiadó, Budapest.
Açıklama
Anahtar Kelimeler
Acrylamide, Bread, LC-MS/MS, Liquid chromatography tandem mass spectrometry
Kaynak
Acta Alimentaria
WoS Q Değeri
Scopus Q Değeri
Q3
Cilt
44
Sayı
2
