Effects of Embryonic Neural Stem Cell Therapy on Oxidative and Nitrosative Stress Biomarkers in Acute and Chronic Spinal Cord Injured Rats
Küçük Resim Yok
Tarih
2009
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Ortadogu Ad Pres & Publ Co
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Objective: Primary trauma to the spinal cord triggers a cascade of cellular and molecular events that promote tissue damage and expansion of the lesion for extended periods following the initial injury. Oxidative and nitrosative stresses play an important role in the progression of spinal cord injury (SCI). The experimental embryonic stem cell (ENSC) therapy is being applied to a wide range of SCIs; however, the effects of the ENSC therapy on oxidative and nitrosative stresses are not clear yet. Therefore, we aimed to investigate the effects of ENSC transplantation on antioxidant enzymes [superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX)], nitric oxide synthase (NOS) and nitric oxide (NO) levels. Material and Methods: Antioxidant enzymes, NOS activities and NO levels were measured by using spectrophotometric methods in spinal cord (SC) tissues of rats 7 (acute group) and 28 (chronic group) days after surgical resections of a 10 mm segment of the cord below T8-T9 and sham-operation. Results: SOD levels of acute or chronic control, damage and therapy groups did not show any considerable differences. NOS activities in acute and chronic injury groups increased as compared to their control groups; similarly NO levels significantly elevated after acute and chronic injury and stem cell therapies. Acute and chronic injured groups had a significantly higher CAT activity than their control groups. GPX activity in the acutely induced injury increased as compared to the acute control groups. The stem cell therapy diminished GPX activity in this group; however, it bad a significantly higher level of GPX activity than acute controls. Conclusion: Stem cell therapy is suggested to cause further increases in oxidative and nitrosative stresses. Application of antioxidants coupled with stem cell therapy can be used for treatment of SCI.
Açıklama
Anahtar Kelimeler
Embryonic stem cells, spinal cord injuries, catalase, nitric oxide
Kaynak
Turkiye Klinikleri Tip Bilimleri Dergisi
WoS Q Değeri
Q4
Scopus Q Değeri
Cilt
29
Sayı
2
