Comparative analysis of XTT assay and xCELLigence system by measuring cytotoxicity of resveratrol in human cancer cell lines [İnsan kanser hücre hatlarında resveratrol sitotoksisitesinin XTT testi ve xCELLigence sistemi ile karşılaştırmalı analizi]

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dc.contributor.authorAtmaca H.
dc.contributor.authorBozkurt E.
dc.contributor.authorKısım A.
dc.contributor.authorUslu R.
dc.date.accessioned2019-10-27T08:20:22Z
dc.date.available2019-10-27T08:20:22Z
dc.date.issued2016
dc.departmentEge Üniversitesien_US
dc.description.abstractObjective: In vitro preliminary oncological and translational studies are mainly based on evaluating the cytotoxic effects of a specific compound on cultured cells. Resveratrol is a commercially available compound which is originally isolated from the roots of white hellebore and later from Polygonum cuspidatum. The objective of the study was to compare cytotoxicity data of Resveratrol from XTT end point assay with a real-time cell based xCELLigence system in terms of accuracy, sensitivity, speed and reproducibility in a panel of human cancer cell lines. Methods: XTT end point assay and real-time cell based xCELLigence system were used to evaluate cytotoxicity. Cytotoxicity results were verified by monitoring cells under phase-contrast microscope which were treated with IC50 values of resveratrol. Results: Resveratrol decreased cell viability in a timeand concentration-dependent manner in all cancer cell lines when tested by both the XTT assay and xCELLigence system. Standard deviations of the xCELLigence data were found to be lower than the data from XTT assay. Conclusion: The data from this study strongly imply that xCELLigence system has higher precision, more enlightening and more reproducible than XTT end point assay. © 2016 Turkish Biochemistry Society. All rights reserved.en_US
dc.identifier.doi10.1515/tjb-2016-0128
dc.identifier.endpage421en_US
dc.identifier.issn0250-4685
dc.identifier.issn0250-4685en_US
dc.identifier.issue6en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage413en_US
dc.identifier.urihttps://doi.org/10.1515/tjb-2016-0128
dc.identifier.urihttps://hdl.handle.net/11454/25622
dc.identifier.volume41en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherTurkish Biochemistry Societyen_US
dc.relation.ispartofTurkish Journal of Biochemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCytotoxicityen_US
dc.subjectHuman cancer cell linesen_US
dc.subjectResveratrolen_US
dc.subjectXCELLigenceen_US
dc.subjectXTTen_US
dc.titleComparative analysis of XTT assay and xCELLigence system by measuring cytotoxicity of resveratrol in human cancer cell lines [İnsan kanser hücre hatlarında resveratrol sitotoksisitesinin XTT testi ve xCELLigence sistemi ile karşılaştırmalı analizi]en_US
dc.typeArticleen_US

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