An acetylcholinesterase biosensor based on a conducting polymer using multiwalled carbon nanotubes for amperometric detection of organophosphorous pesticides

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dc.contributor.authorKesik, Melis
dc.contributor.authorKanik, Fulya Ekiz
dc.contributor.authorTuran, Janset
dc.contributor.authorKolb, Marit
dc.contributor.authorTimur, Suna
dc.contributor.authorBahadir, Muefit
dc.contributor.authorToppare, Levent
dc.date.accessioned2019-10-27T22:12:21Z
dc.date.available2019-10-27T22:12:21Z
dc.date.issued2014
dc.departmentEge Üniversitesien_US
dc.description.abstractA novel amperometric biosensor based on a conducting polymer using multi walled carbon nanotube modified electrode was developed for detection of organophosphorus pesticides. Acetylcholinesterase (AChE) was successfully immobilized by covalent linkage on the modified graphite electrode. Carbon nanotubes were functionalized by electrochemical treatment. A conducting polymer; poly(4-( 2,5-di(thiophen-2-yl)-1H-pyrrol-1-yl)benzenamine) (poly( SNS-NH2)) was synthesized via electropolymerization to examine its matrix properties for biomolecule immobilization. This strategy enhanced electron transfer rate at a lower potential (+100 mV vs. Ag reference) and catalyzed electrochemical oxidation of acetylthiocholine effectively. Scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), contact angle measurements and electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) techniques were used to monitor changes in surface morphologies and electrochemical characterizations. The proposed biosensor design offered a fast response time (6 s), a wide linear range (0.05 mM and 8.00 mM) and a low detection limit (0.09 mM) with a high sensitivity (24.16 mu AmM-1 cm(-2)) for acetylthiocholine. The inhibition responses of paraoxon, parathion and chlorfenvinphos on the enzymatic activity of AChE were detected. The fabricated biosensor was tested for the detection of pesticides in fortified tap water samples. The results were found to be in good agreement with the ones determined by HPLC/DAD technique. (C) 2014 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipGerman Federal Ministry of Education and Research (BMBF WTZ project) [TUR 10-003, 01DL12015]en_US
dc.description.sponsorshipThe authors acknowledge the financial support from the German Federal Ministry of Education and Research (BMBF WTZ project TUR 10-003) grant number (01DL12015).en_US
dc.identifier.doi10.1016/j.snb.2014.08.058
dc.identifier.endpage49en_US
dc.identifier.issn0925-4005
dc.identifier.issn0925-4005en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage39en_US
dc.identifier.urihttps://doi.org/10.1016/j.snb.2014.08.058
dc.identifier.urihttps://hdl.handle.net/11454/49386
dc.identifier.volume205en_US
dc.identifier.wosWOS:000343117600006en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevier Science Saen_US
dc.relation.ispartofSensors and Actuators B-Chemicalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBiosensoren_US
dc.subjectAcetylcholinesteraseen_US
dc.subjectPesticidesen_US
dc.subjectAmperometryen_US
dc.titleAn acetylcholinesterase biosensor based on a conducting polymer using multiwalled carbon nanotubes for amperometric detection of organophosphorous pesticidesen_US
dc.typeArticleen_US

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