Development and in vitro Evaluation of Voriconazole Nanoparticle Formulation for Mucosal Application

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dc.contributor.authorRencber, Seda
dc.contributor.authorKaravana, Sinem Yaprak
dc.date.accessioned2019-10-27T10:04:00Z
dc.date.available2019-10-27T10:04:00Z
dc.date.issued2018
dc.departmentEge Üniversitesien_US
dc.description.abstractObjectives: This study aimed to prepare and evaluate mucoadhesive nanoparticle formulations of voriconazole, an antifungal drug, for mucosal application. It was also aimed to develop and validate a HPLC method of voriconazole. Materials and Methods: In this study, mucoadhesive nanoparticles containing voriconazole were prepared using a coating polymer of chitosan. The obtained nanoparticles were characterized via particle size, polydispersity index, zeta potential measurement, and mucoadhesion studies. Drug loading capacity was tested for determination of the voriconazole amount in the nanoparticles. In vitro drug release was also examined. The HPLC method was validated for linearity, accuracy, precision (repeatability and reproducibility), specificity, stability, limits of detection (LOD), and limit of quantification (LOQ). Results: In vitro characterization results of the mucoadhesive nanoparticle formulation containing voriconazole was found to be appropriate with a small particle size of 217.1 +/- 4.2 nm, a narrow polydispersity index of 0.335 +/- 0.042, 99.052 +/- 0.424% drug loading, and a positive zeta potential value of +26.82 +/- 0.4 mV. According to a mucoadhesive study, it can be concluded that the nanoparticle was able to interact with mucin due to ionic interaction. Also, the turbidity of nanoparticle/mucin dispersion was higher than the turbidity of mucin dispersion itself. Based on the in vitro drug release, no burst effect was observed, indicating that voriconazole was homogeneously dispersed in the nanoparticle dispersion and that no significant amount of drug was adsorbed onto the nanoparticle surface. The release was found to follow a non-Fickian diffusion mechanism with first-order drug release. The proposed HPLC method was simple, highly sensitive with good linearity, accurate, precise, specific, and stable, showing that the method is useful for routine quality control. Conclusion: This study has shown that the mucoadhesive nanoparticle formulation containing voriconazole reported here is a promising candidate for the local treatment of mucosal diseases. The developed HPLC method can be succesfully applied to pharmaceutical preparations containing voriconazole.en_US
dc.description.sponsorshipResearch Foundation of Ege UniversityEge University [12/ECZ/034]en_US
dc.description.sponsorshipThe authors gratefully acknowledge Research Foundation of Ege University (12/ECZ/034) for their financial support.en_US
dc.identifier.doi10.4274/tjps.54265
dc.identifier.endpage148en_US
dc.identifier.issn1304-530X
dc.identifier.issn1304-530Xen_US
dc.identifier.issue2en_US
dc.identifier.startpage142en_US
dc.identifier.urihttps://doi.org/10.4274/tjps.54265
dc.identifier.urihttps://hdl.handle.net/11454/30186
dc.identifier.volume15en_US
dc.identifier.wosWOS:000438973100005en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherTurkish Pharmacists Assocen_US
dc.relation.ispartofTurkish Journal of Pharmaceutical Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectVoriconazoleen_US
dc.subjectmucoadhesive nanoparticleen_US
dc.subjectchitosanen_US
dc.subjectlocal applicationen_US
dc.subjectHPLCen_US
dc.titleDevelopment and in vitro Evaluation of Voriconazole Nanoparticle Formulation for Mucosal Applicationen_US
dc.typeArticleen_US

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