Investigation of usability of fluorescent and radiolabeled liquid penetrant as a nondestructive testing agent

The fluorescent penetrant inspection (FPI) is a non-destructive method used for identifying and locating surface discontinuities. Traditional dye penetrant testing of surface cracks in metals has limited sensitivies to micro-scale surface defects. More recently, studies mainly aimed at developing a new generation penetrants which will be able to detect the micro-scale cracks in surface. In accordance with these purpose; detection of defects in metals can be inspected with fluorimetric and radioisotopic via Fluorescein Isothiocyonate (FITC) conjugation of99mTc radiolabeled Escherichia coli (E.Coli). In this study, bacteria were conjugated with FITC which is a fluorescent dye in order to give fluorescent properties to E.Coli and then radiolabeled with99mTc radionuclide by tin chloride reduction method and surface defects were detected by both fluorimetric and radioisotopic methods. This study will contribute to recent researches on the detection of surface defects or damage in micro scale by both these methods and will be gained a new perspective on existing applications. There have been very few papers related to our study. From this point of view, these studies will accelerate R&D activities where advanced technology and materials are used. This paper will also provide to the literature support for the recent years of organic bacterial penetrant studies and related researches. © 2017 Slovenian Society for Non-Destructive Testing. All rights reserved.