Inhibitory activities of Helichrysum taxa on mammalian type I DNA topoisomerase

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dc.contributor.authorKucukoglu, O.
dc.contributor.authorOzturk, B.
dc.contributor.authorKamataki, T.
dc.contributor.authorTopcu, Z.
dc.date.accessioned2019-10-27T19:19:16Z
dc.date.available2019-10-27T19:19:16Z
dc.date.issued2006
dc.departmentEge Üniversitesien_US
dc.description.abstractDNA topoisomerases are essential enzymes that regulate the conformational changes in DNA topology by catalyzing the concerted breakage and rejoining of DNA strands during normal cellular growth. During the past few years, there has been considerable pharmacological interest in these enzymes because the inhibitors of DNA topoisomerases represent a major class of anticancer drugs. In this study, we investigated the effects of the extracts, prepared from a number of Helichrysum taxa, on mammalian DNA topoisomerase I via in vitro supercoil relaxation assays using plasmid substrate, pBR322. Cytotoxic alkaloid, camptothecin, an inhibitor of eukaryotic topoisomerase I, was used as reference compound throughout the assays. Quantitative comparisons of the supercoiled and relaxed DNA band intensities on agarose gels suggested that the extracts from H. pallasii (Sprengel) Ledeb., H. armenium DC subsp. araxinum (Kirp.) Takht, and H. plicatum DC subsp. plicatum manifested a considerable inhibition on the enzyme in a dose-dependent manner. The inhibition of mammalian DNA topoisomerase I by Helichrysum extracts is a significant result because they can be potential sources of anticancer drugs.en_US
dc.identifier.doi10.1080/13880200600685972
dc.identifier.endpage193en_US
dc.identifier.issn1388-0209
dc.identifier.issn1744-5116
dc.identifier.issn1388-0209en_US
dc.identifier.issn1744-5116en_US
dc.identifier.issue3en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage189en_US
dc.identifier.urihttps://doi.org/10.1080/13880200600685972
dc.identifier.urihttps://hdl.handle.net/11454/38718
dc.identifier.volume44en_US
dc.identifier.wosWOS:000237848000007en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofPharmaceutical Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectcancer chemotherapyen_US
dc.subjectflavonoiden_US
dc.subjectHelichrysumen_US
dc.subjectmammalian DNA topoisomerase Ien_US
dc.titleInhibitory activities of Helichrysum taxa on mammalian type I DNA topoisomeraseen_US
dc.typeArticleen_US

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