Octaarginine functionalized nanoencapsulated system: In vitro and in vivo evaluation of bFGF loaded formulation for wound healing

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dc.authoridkeselik, erdi/0000-0002-1793-7719
dc.authoridAkbaba, Hasan/0000-0001-9273-6346
dc.authoridErel-Akbaba, Gulsah/0000-0003-3287-5277
dc.authorscopusid57200229272
dc.authorscopusid57190564982
dc.authorscopusid57219405585
dc.authorscopusid6602887297
dc.authorscopusid35774636300
dc.authorscopusid26643347900
dc.authorwosidkeselik, erdi/C-8954-2018
dc.contributor.authorErel-Akbaba, Gulsah
dc.contributor.authorAkbaba, Hasan
dc.contributor.authorKeselik, Erdi
dc.contributor.authorBahceci, Selen Akyol
dc.contributor.authorSenyigit, Zeynep
dc.contributor.authorTemiz, Tijen Kaya
dc.date.accessioned2023-01-12T19:55:14Z
dc.date.available2023-01-12T19:55:14Z
dc.date.issued2022
dc.departmentN/A/Departmenten_US
dc.description.abstractDespite all treatment efforts, diabetic wounds are still a major health concern that needs to be solved. Growth factor-based therapeutic approaches offer promising results for wound healing therapy. However, their high sensitivity to proteolysis causes repetitive application, and associated high cost makes it necessary to develop an effective delivery system. In this study, an innovative approach for protein delivery was developed and evaluated in terms of wound healing activity. For this purpose, basic fibroblast growth factor was first encapsulated into chitosan and alginate based polymeric system. Then, a cell penetrating peptide, octaarginine, was incorporated onto the system to achieve a higher therapeutic effect. The characterization studies were performed, and the optimal nanoparticle system was dispersed in hydroxypropyl methylcellulose for providing skin application. The in vitro and in vivo efficiency of the system was also evaluated. According to the results, the obtained gel formulation is safe for the applied doses up to 150 mg/mL and significantly promotes fibroblast cell migration in 48 h. Furthermore, in vivo studies revealed that the diabetic wound healing efficiency of basic fibroblast growth factor nanoencapsulated gel system significantly accelerates wound healing. Moreover, by functionalizing this system with octaarginine, epithelization, granulation of tissue formation, and treatment efficacy were promoted. In conclusion, this work designs a suitable cell penetrating peptide functionalized nanoencapsulated system for growth factor delivery, which provides a promising therapeutic potential for hard-to-heal skin lesions, particu-larly diabetic wounds.en_US
dc.description.sponsorshipIzmir Katip Celebi University, Scientific Research Projects Coordinatorship [2019-ONAP-ECZF-0002]en_US
dc.description.sponsorshipThe authors would like to thank Sinem Yaprak Karavana from Ege University, Faculty of Pharmacy for her help with rheological studies and F. Emrah SOYLU from Ege University, Experimental Animal Research Center (Izmir, Turkey) for his help with animal studies. This project was supported by grant from Izmir Katip Celebi University, Scientific Research Projects Coordinatorship (Project Number: 2019-ONAP-ECZF-0002) .en_US
dc.identifier.doi10.1016/j.jddst.2022.103343
dc.identifier.issn1773-2247
dc.identifier.issn2588-8943
dc.identifier.issn1773-2247en_US
dc.identifier.issn2588-8943en_US
dc.identifier.scopus2-s2.0-85128449281en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1016/j.jddst.2022.103343
dc.identifier.urihttps://hdl.handle.net/11454/76641
dc.identifier.volume71en_US
dc.identifier.wosWOS:000802911100004en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofJournal Of Drug Delivery Science And Technologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectOctaarginineen_US
dc.subjectChitosanen_US
dc.subjectNanoencapsulationen_US
dc.subjectWound healingen_US
dc.subjectFibroblast growth factoren_US
dc.subjectIn vivo protein deliveryen_US
dc.subjectOral Deliveryen_US
dc.subjectGrowth-Factoren_US
dc.subjectNanoparticlesen_US
dc.subjectCurcuminen_US
dc.titleOctaarginine functionalized nanoencapsulated system: In vitro and in vivo evaluation of bFGF loaded formulation for wound healingen_US
dc.typeArticleen_US

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