Determination of sialic acids in the nervous system of silkworm (Bombyx mori L.): effects of aging and development

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dc.contributor.authorSoya, Seckin
dc.contributor.authorSahar, Umut
dc.contributor.authorYikilmaz, Mehmet Salih
dc.contributor.authorKaracali, Sabire
dc.date.accessioned2019-10-27T11:21:48Z
dc.date.available2019-10-27T11:21:48Z
dc.date.issued2017
dc.departmentEge Üniversitesien_US
dc.description.abstractSialic acids mainly occur as components on cell surface glycoproteins and glycolipids. They play a major role in the chemical and biological diversity of glycoconjugates. Although sialic acids exhibit great structural variability in vertebrates, glycoconjugates with sialic acids have also been determined in small amounts in invertebrates. It has been suggested that sialic acids play important roles in the development and function of the nervous system. Despite Bombyx mori being a model organism for the investigation of many physiological processes, sialic acid changes in its nervous system have not been examined during development and aging. Therefore, in this study we aimed to determine sialic acid changes in the nervous system of Bombyx mori during development and aging processes. Liquid chromatography-mass spectrometry (LC-MS) and lectin immunohistochemistry were carried out in order to find variations among different developmental stages. Developmental stages were selected as 3rd instar (the youngest) and 5th larval instar (young), motionless prepupa (the oldest) and 13-day-old pupa (adult development). At all stages, only Neu5Ac was present, however, it dramatically decreased during the developmental and aging stages. On the other hand, an increase was observed in the amount of Neu5Ac during the pupal stage. In immunohistochemistry experiments with Maackia amurensis agglutinin (MAA) and Sambucus nigra agglutinin (SNA) lectins, the obtained staining was consistent with the obtainedLC-MS results. These findings indicate that sialic acids are abundant at the younger stages but that they decrease in the insect nervous system during development and aging, similarly as in mammals.en_US
dc.identifier.doi10.2298/ABS160401117S
dc.identifier.endpage378en_US
dc.identifier.issn0354-4664
dc.identifier.issn1821-4339
dc.identifier.issn0354-4664en_US
dc.identifier.issn1821-4339en_US
dc.identifier.issue2en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage369en_US
dc.identifier.urihttps://doi.org/10.2298/ABS160401117S
dc.identifier.urihttps://hdl.handle.net/11454/32958
dc.identifier.volume69en_US
dc.identifier.wosWOS:000402556600020en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherInst Bioloska Istrazivanja Sinisa Stankovicen_US
dc.relation.ispartofArchives of Biological Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectSialic acidsen_US
dc.subjectnervous systemen_US
dc.subjectcapLC-ESI-MS/MSen_US
dc.subjectlectin immunohistochemistryen_US
dc.subjectdevelopmenten_US
dc.subjectagingen_US
dc.titleDetermination of sialic acids in the nervous system of silkworm (Bombyx mori L.): effects of aging and developmenten_US
dc.typeArticleen_US

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