Detection of Methicillin-Resistant Staphylococcus Aureus Bacteria Using Liquid Crystals

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dc.contributor.authorKemiklioglu, Emine
dc.contributor.authorTuncgovde, Ebru Busra
dc.date.accessioned2023-01-12T20:12:02Z
dc.date.available2023-01-12T20:12:02Z
dc.date.issued2022
dc.departmentN/A/Departmenten_US
dc.description.abstractMethicillin-resistant Staphylococcus aureus (MRSA) is an essential pathogen for public health and this bacteria commonly cause serious infectious in humans. In recent years, MRSA bacteria are detected by the bacterial culture and nucleic acid-based methods which are time-consuming and labor-intensive. In this study, a novel liquid crystal (LC)-based biosensing system was developed to overcome these limitations. The objective of this study was to detect the presence of MRSA bacteria which prepared within the isotonic water and phosphate buffer saline (PBS). In this system, the binding of MRSA bacteria to the dimettw loctadecyl [3-(trimethoxysilyl) propyl] ammonium chloride (DMOAP)-coated surface disrupted the orientation of I Cs. triggering a transition from a homeotropic orientation to a random one. This transition in the orientation of the LCs was observed as a change from a dark optical LC image to a bright one under a polarized light microscope (POM) and the reflection values of LC molecules were determined by using a spectrometer. Through this sensing mechanism, MRSA bacteria prepared within the isotonic water was detected ranging from the 9.2x10(3) CFU/mL to 9.2x10(7) CFU/mL concentration. Furthermore. Ni RSA bacteria prepared in PBS was detected in the concentration range of 7.1x10(4) CFU/mL to 7.1x10(8) CFU/ml by using this system.en_US
dc.identifier.doi10.2339/politeknik.934565
dc.identifier.issn1302-0900
dc.identifier.issn2147-9429
dc.identifier.issn1302-0900en_US
dc.identifier.issn2147-9429en_US
dc.identifier.urihttps://doi.org/10.2339/politeknik.934565
dc.identifier.urihttps://hdl.handle.net/11454/78235
dc.identifier.wosWOS:000777847300001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherGazi Univen_US
dc.relation.ispartofJournal Of Polytechnic-Politeknik Dergisien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBiosensoren_US
dc.subjectMRSA bacteriaen_US
dc.subjectliquid crystalen_US
dc.subjectBiosensorsen_US
dc.titleDetection of Methicillin-Resistant Staphylococcus Aureus Bacteria Using Liquid Crystalsen_US
dc.typeArticleen_US

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