Characterisation of a thermostable and proteolysis resistant phytase fromPenicillium polonicumMF82 associated with the marine spongePhorbassp.

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dc.contributor.authorKalkan, Saban Orcun
dc.contributor.authorBozcal, Elif
dc.contributor.authorTuna, Elif Esin Hames
dc.contributor.authorUzel, Atac
dc.date.accessioned2020-12-01T11:59:18Z
dc.date.available2020-12-01T11:59:18Z
dc.date.issued2020
dc.departmentEge Üniversitesien_US
dc.description.abstractPhytases are widely used in human and animal nutrition, aquaculture, soil amendment, and in the production of lower myo-inositol phosphates for clinical purposes. Some of these applications, especially feed industry require robust enzymes. Since the marine environments are less studied compared to terrestrial environments, we evaluated the extracellular phytase activity of 110 marine derived filamentous fungal (MDFF) strains previously isolated from sponge and sediment samples of the Turkey. MDFF strains were qualitatively screened for their extracellular phytase activities andP. polonicumMF82 phytase was further characterized following partial purification. Optimum pH and temperature were determined as 5.5 and 60 degrees C respectively. A significant relative phytase activity was observed in the presence of urea and acetone. However, there was no phytase activity followed by the treatment with Triton X-100 and Tween 80. Characterization studies revealed thatP. polonicumMF82 phytase has superior properties for industrial use including wide pH and temperature range for activity, high optimum activity temperature, high thermal and pH stability, resistance to many enzyme inhibitors including various heavy metals, denaturants, detergents, proteases and organic solvents. Phytase extracellularly produced byP. polonicumMF82 strain presents a good candidate for commercial applications. This study demonstrates that the MDFF strains are prolific sources for phytase and presents the first report about the production and characterization of the phytase from a marine-derivedP. polonicumstrain.en_US
dc.description.sponsorshipEge University Scientific Projects FoundationsEge University [14-Fen-004]; Scientific and Technical Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK); TUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [112T047]en_US
dc.description.sponsorshipWe gratefully acknowledge the support for this research by the Ege University Scientific Projects Foundations, Project No; 14-Fen-004.; This work was also supported in part by the Scientific and Technical Research Council of Turkey (TUBITAK) by providing scholarship to S. Orcun Kalkan with the 2211-C programme and Elif Bozcal was a scholar provided by TUBITAK research fund (Project No: 112T047).en_US
dc.identifier.doi10.1080/10242422.2020.1785434
dc.identifier.endpage479en_US
dc.identifier.issn1024-2422
dc.identifier.issn1029-2446
dc.identifier.issn1024-2422en_US
dc.identifier.issn1029-2446en_US
dc.identifier.issue6en_US
dc.identifier.scopus2-s2.0-85087503276en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage469en_US
dc.identifier.urihttps://doi.org/10.1080/10242422.2020.1785434
dc.identifier.urihttps://hdl.handle.net/11454/62224
dc.identifier.volume38en_US
dc.identifier.wosWOS:000549499600001en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofBiocatalysis and Biotransformationen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectMarine-derived fungien_US
dc.subjectPenicillium polonicumen_US
dc.subjectPhorbas spen_US
dc.subjectphytaseen_US
dc.subjectthermostableen_US
dc.subjectprotease resistanten_US
dc.titleCharacterisation of a thermostable and proteolysis resistant phytase fromPenicillium polonicumMF82 associated with the marine spongePhorbassp.en_US
dc.typeArticleen_US

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