Brilliant green sequestered poly(amic) acid film for dual-mode detection: Fluorescence and electrochemical enzymatic biosensor

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dc.contributor.authorMaiga, Mohomodou
dc.contributor.authorYazgan, Idris
dc.contributor.authorKariuki, Victor M.
dc.contributor.authorDemirkol, Dilek Odaci
dc.contributor.authorSadik, Omowunmi A.
dc.contributor.authorTimur, Suna
dc.date.accessioned2019-10-27T10:42:16Z
dc.date.available2019-10-27T10:42:16Z
dc.date.issued2018
dc.departmentEge Üniversitesien_US
dc.description.abstractHerein, we report a facile technique to fabricate fluorescent polymeric structure, brilliant-green sequestered poly(amic) acid film (BG-PAA), as enzyme support-material for biosensor applications. The structure and fluorescence properties of the fabricated BG-PAA membrane were investigated using H-1 NMR, scanning electron microscopy (SEM) and fluorescence microscopy. Glucose oxidase (GOx) was used as a model enzyme that immobilized on BG-PAA membrane using glutaraldehyde. The created BGPAA/GOx membrane was utilized to fabricate dual-purpose as fluorescent and electrochemical biosensors for the determination of glucose in beverages. Utilization of the dissolved oxygen in GOx mediated glucose oxidation resulted in alteration of the measured fluorescence and current intensity. In the fluorescence assay, the decreased concentration of oxygen causes the increased fluorescence intensity due to the elimination of quenching effect of oxygen on BG fluorescence. In amperometric assay, decrease in oxygen concentration was followed at -0.7 V. After optimization of working conditions linearity, limit of detection and repeatability of system were determined. Finally, BG-PAA/GOx was tested to analyze glucose in beverages. (C) 2017 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipBAGEP Award of the Science Academy; Ege UniversityEge University [15-FEN-022]; Ege University Aliye Uster FoundationEge University; Turkish government scholarship program "Turkiye burslari"; Melinda-Bill Gates-foundation; National Science Foundation/Bill & Melinda Gates Foundation [IOS-154394]en_US
dc.description.sponsorshipThis work was supported by the BAGEP Award of the Science Academy. The authors would like to thank scientific research project (Ege University Project No: 15-FEN-022) and Ege University Aliye Uster Foundation. Mohomodou Maiga thanks Turkish government scholarship program "Turkiye burslari". Dr. Idris Yazgan thanks to Melinda-Bill Gates-foundation for funding the Post-Doctoral studies. The authors acknowledge the National Science Foundation/Bill & Melinda Gates Foundation Grant #IOS-154394.en_US
dc.identifier.doi10.1016/j.snb.2017.10.002
dc.identifier.endpage78en_US
dc.identifier.issn0925-4005
dc.identifier.issn0925-4005en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage71en_US
dc.identifier.urihttps://doi.org/10.1016/j.snb.2017.10.002
dc.identifier.urihttps://hdl.handle.net/11454/30669
dc.identifier.volume256en_US
dc.identifier.wosWOS:000414971100009en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevier Science Saen_US
dc.relation.ispartofSensors and Actuators B-Chemicalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPoly(amic) aciden_US
dc.subjectFluorescence detectionen_US
dc.subjectAmperometric detectionen_US
dc.subjectDual-mode detectionen_US
dc.subjectEnzymatic sensorsen_US
dc.titleBrilliant green sequestered poly(amic) acid film for dual-mode detection: Fluorescence and electrochemical enzymatic biosensoren_US
dc.typeArticleen_US

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