Astragaloside IV and Cycloastragenol Production Capacity of Astragalus trojanus Calli

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dc.contributor.authorNartop, Pinar
dc.contributor.authorGurel, Aynur
dc.contributor.authorAkgun, Ismail Hakki
dc.contributor.authorBedir, Erdal
dc.date.accessioned2019-10-27T22:30:08Z
dc.date.available2019-10-27T22:30:08Z
dc.date.issued2015
dc.departmentEge Üniversitesien_US
dc.description.abstractAstragalus species are medicinal plants which produce valuable secondary metabolites, especially cycloartane-type glycosides. In this study, stem and leaf explants of Astragalus trojanus were subjected to different plant growth regulators, environmental conditions and media compositions to identify their callus responses. Stem and leaf explants were cultured in Murashige and Skoog (MS) and woody plant (WPM) media supplemented with different concentrations of kinetin, naphthalene acetic acid, 2,4-dichlorophenoxyacetic acid, thidiazurone and indol acetic acid under two light intensities (1000 and 4000 lux) and also in dark conditions. Both MS and WPM media triggered callus regeneration. Although, callus regeneration was observed on both stem and leaf explants, callus biomass accumulation on stem explants were higher. Addition of 100 mu g/L selenium and doubled concentration of WPM vitamins enhanced callus biomass on stem explants under dark conditions. Stem explants also regenerated shoots at high frequencies (up to 93%), especially in kinetin added media. Astragaloside IV and cycloastragenol accumulation efficiencies were determined in calli tissues. The highest astragaloside IV production (3.5 mu g/mg) was found in callus tissue regenerated from stem explants in D1 medium, whereas the highest cycloastragenol accumulation (4.8 mu g/mg) was detected in callus tissue regenerated from stem explants in N2 medium.en_US
dc.description.sponsorshipScientific and Technical Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [TOVAG-109O62]en_US
dc.description.sponsorshipThe financial support of The Scientific and Technical Research Council of Turkey (TUBITAK) (Project No. TOVAG-109O627) is gratefully acknowledged.en_US
dc.identifier.endpage61en_US
dc.identifier.issn1307-6167
dc.identifier.issn1307-6167en_US
dc.identifier.issue1en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage49en_US
dc.identifier.urihttps://hdl.handle.net/11454/51209
dc.identifier.volume9en_US
dc.identifier.wosWOS:000350973300004en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherAcg Publicationsen_US
dc.relation.ispartofRecords of Natural Productsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAstragalus trojanusen_US
dc.subjectcallusen_US
dc.subjectshoot regenerationen_US
dc.subjectastragaloside IVen_US
dc.subjectcycloastragenolen_US
dc.subjectplant growth regulatorsen_US
dc.titleAstragaloside IV and Cycloastragenol Production Capacity of Astragalus trojanus Callien_US
dc.typeArticleen_US

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