Determination of Carbidopa, Levodopa, and Droxidopa by High-Performance Liquid Chromatography-Tandem Mass Spectrometry

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dc.contributor.authorOzdokur, Kemal Volkan
dc.contributor.authorEngin, Esra
dc.contributor.authorYengin, Cigdem
dc.contributor.authorErtas, Hasan
dc.contributor.authorErtas, F. Nil
dc.date.accessioned2019-10-27T10:47:02Z
dc.date.available2019-10-27T10:47:02Z
dc.date.issued2018
dc.departmentEge Üniversitesien_US
dc.description.abstractModern treatments of Parkinson's disease include the use of carbidopa and droxidopa along with levodopa that demonstrates the necessity for their binary and simultaneous determination in pharmaceutical tablets and biological matrices. This study presents a rapid, accurate, and precise method for the simultaneous determination of these analytes by liquid chromatography- tandem mass spectrometry method for the first time. Accordingly, performances of two types of analytical columns, namely, octadecylsilane and hydrophilic interaction liquid chromatographic columns, were tested and better resolution was obtained with the former. The pH of aqueous mobile phase (0.5% formic acid) was adjusted to 2.3 to ensure the analytes were in molecular form. Calibration graphs were obtained using multiple reaction mode and detection limits were in the region from 0.002 to 0.007 mu M for both columns. The method developed was applied for quality control samples and tablets and satisfactory recovery values from 100 to 106% were obtained.en_US
dc.description.sponsorshipTUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [114 Z 153]en_US
dc.description.sponsorshipThis project was supported by TUBITAK with project number 114 Z 153.en_US
dc.identifier.doi10.1080/00032719.2017.1316283
dc.identifier.endpage82en_US
dc.identifier.issn0003-2719
dc.identifier.issn1532-236X
dc.identifier.issn0003-2719en_US
dc.identifier.issn1532-236Xen_US
dc.identifier.issue01.Feben_US
dc.identifier.startpage73en_US
dc.identifier.urihttps://doi.org/10.1080/00032719.2017.1316283
dc.identifier.urihttps://hdl.handle.net/11454/31374
dc.identifier.volume51en_US
dc.identifier.wosWOS:000416519900006en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Incen_US
dc.relation.ispartofAnalytical Lettersen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCarbidopaen_US
dc.subjectdroxidopaen_US
dc.subjecthigh-performance liquid chromatography-tandem mass spectrometry (HPLCMS)en_US
dc.subjecthydrophilic interaction liquid chromatography (HILIC)en_US
dc.subjectlevodopaen_US
dc.titleDetermination of Carbidopa, Levodopa, and Droxidopa by High-Performance Liquid Chromatography-Tandem Mass Spectrometryen_US
dc.typeArticleen_US

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