Focusing on a new biohydrogen production strategy (HS-HS) using Chlamydomonas reinhardtii mutants: The effects of mutation points on biohydrogen production capacity

In this study, we investigated biohydrogen production potentials of Chlamydomonas reinhardtii strains as CC124, D1 mutants, PGRL1 mutants, mitochondrial mutant AOX, and control strains as WT and T-1. Among all cultures, D240-41, PGRL1x?nd4 Véro and PGRL1 mt (+) mutants produced the highest amount biohydrogen as 150 ± 2, 170 ± 2 and 140 ± 2 mL H2 L?1, respectively during TAP-TAP (-S) strategy. Then, the three biohydrogen production strategies (TAP-TAP (-S), TAP-HS, and HS-HS) were compared to each other by using the CC124 strain. The culture was capable of producing biohydrogen during the performing TAP-TAP (-S), and HS-HS strategies. The voltage differences were observed at 225 ± 1 mV and 24 ± 1 mV, respectively. Then, D240-41, PGRL1x?nd4 Véro and PGRL1 mt (+) cultures were evaluated according to HS-HS strategy. The D240-41 strain caused the highest difference in the voltage measurement, which was attained at 80 ± 1 mV. As a result, all selected strains were able to produce hydrogen during this new HS-HS strategy. © 2023 Hydrogen Energy Publications LLC