Immobilization of porcine pancreatic lipase on glycidyl methacrylate grafted poly vinyl alcohol

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dc.contributor.authorKartal, F.
dc.contributor.authorAkkaya, A.
dc.contributor.authorKilinc, A.
dc.date.accessioned2019-10-27T20:52:10Z
dc.date.available2019-10-27T20:52:10Z
dc.date.issued2009
dc.departmentEge Üniversitesien_US
dc.description.abstractCraft copolymerization of glycidyl methacrylate (GMA) on to polyvinyl alcohol (PVA) using benzophenone (BP) as initiator was carried out. Grafted PVA was used as carrier for pancreatic lipase immobilization. The effects of GMA and BP concentrations as well as grafting reaction times on grafting yields and activities of the immobilized lipase were determined. The influence of enzyme concentrations was also studied. The optimal conditions for the grafting reaction were: 1 h at 15 mM BP and 2.3 M GMA, the optimum enzyme concentration for immobilization was 1 mg/ml. After optimization of the immobilization process a physical and chemical characterization of the immobilized enzyme was performed. Furthermore, the thermal, pH, storage and operational stability of the immobilized enzyme in comparison to the free form was tested. (c) 2008 Elsevier B.V. All rights reserved.en_US
dc.identifier.doi10.1016/j.molcatb.2008.06.016
dc.identifier.endpage61en_US
dc.identifier.issn1381-1177
dc.identifier.issn1873-3158
dc.identifier.issn1381-1177en_US
dc.identifier.issn1873-3158en_US
dc.identifier.issue01.Apren_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage55en_US
dc.identifier.urihttps://doi.org/10.1016/j.molcatb.2008.06.016
dc.identifier.urihttps://hdl.handle.net/11454/43198
dc.identifier.volume57en_US
dc.identifier.wosWOS:000264899000010en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofJournal of Molecular Catalysis B-Enzymaticen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectLipaseen_US
dc.subjectImmobilizationen_US
dc.subjectPolyvinyl alcoholen_US
dc.subjectGraft polymerizationen_US
dc.subjectGlycidyl methacrylateen_US
dc.subjectBenzophenoneen_US
dc.titleImmobilization of porcine pancreatic lipase on glycidyl methacrylate grafted poly vinyl alcoholen_US
dc.typeArticleen_US

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