Labeling bombesin-like peptide with Tc-99m via hydrazinonicotinamide: description of optimized radiolabeling conditions

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dc.contributor.authorLambrecht, F. Yurt
dc.contributor.authorDurkan, K.
dc.contributor.authorBayrak, E.
dc.date.accessioned2019-10-27T21:17:29Z
dc.date.available2019-10-27T21:17:29Z
dc.date.issued2010
dc.departmentEge Üniversitesien_US
dc.description.abstractBombesin (BNN)-like peptides have very high binding affinity for the gastrin-releasing peptide (GRP) receptor. The goal of the current study was to optimize the labeling conditions of a new Tc-99m-radiolabeled BNN-like peptide based on the bifunctional chelating ligand HYNIC using different co-ligands (EDDA and tricine). The radiolabeling conditions (pH, amount of co-ligand, amount of stannous chloride, temperature and reaction time) for newly-formed Tc-99m-tricine-HYNIC-Q-Litorin and Tc-99m-EDDA-HYNIC-Q-Litorin were optimized and evaluated by RHPLC and RTLC. Radiochemical yields for Tc-99m-tricine-HYNIC-Q-Litorin and Tc-99m-EDDA-HYNIC-Q-Litorin were 98.0 +/- A 1.7 and 97.5 +/- A 2.5%, respectively. When EDDA was used as co-ligand, the labeling of Tc-99m-EDDA-HYNIC-Q-Litorin was optimal in the following reaction mixture: HYNIC-peptide: EDDA: 10 mu g/5 mg, pH 3, SnCl2 concentration: 12 mu g/0.1 mL, reaction temperature: 100 A degrees C, reaction time: 15 min. Besides, the optimum conditions were HYNIC-peptide:tricine: 10 mu g/50 mg, pH 5, SnCl2 concentration: 12 mu g/0.1 mL, reaction temperature: 100 A degrees C, reaction time: 15 min for preparing Tc-99m-tricine-HYNIC-Q-Litorin. The manufactured Tc-99m-HYNIC-Q-Litorin conjugates may offer new possibilities for imaging cancer cells expressing bombesin receptors.en_US
dc.description.sponsorshipScientific and Technological Research Council of TurkeyTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [108S200]en_US
dc.description.sponsorshipThe authors gratefully acknowledge the financial support received from the Scientific and Technological Research Council of Turkey, Scientific Project (TUBITAK Number 108S200).en_US
dc.identifier.doi10.1007/s10967-010-0530-8
dc.identifier.endpage545en_US
dc.identifier.issn0236-5731
dc.identifier.issn0236-5731en_US
dc.identifier.issue3en_US
dc.identifier.startpage539en_US
dc.identifier.urihttps://doi.org/10.1007/s10967-010-0530-8
dc.identifier.urihttps://hdl.handle.net/11454/43825
dc.identifier.volume284en_US
dc.identifier.wosWOS:000276910200008en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofJournal of Radioanalytical and Nuclear Chemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectHYNIC-BNN-like peptideen_US
dc.subjectLitorinen_US
dc.subjectGastrin-releasing peptideen_US
dc.subjectTc-99men_US
dc.subjectCo-liganden_US
dc.titleLabeling bombesin-like peptide with Tc-99m via hydrazinonicotinamide: description of optimized radiolabeling conditionsen_US
dc.typeArticleen_US

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