Purification of phycocyanin from isolated and identified hot spring cyanobacteria

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dc.contributor.authorDemirel, Zeliha
dc.contributor.authorSukatar, Atakan
dc.date.accessioned2019-10-27T09:44:32Z
dc.date.available2019-10-27T09:44:32Z
dc.date.issued2019
dc.departmentEge Üniversitesien_US
dc.description.abstractPhycocyanin is a phycobiliprotein used as a healthy food additive as well as an ingredient in cosmetic dye preparation. In this study, we attempted molecular and morphological identification of three thermophilic filamentous cyanobacteria and also explored a method to separate and purify thermostable C-phycocyanin of analytical grade from them for biotechnological applications. Cyanobacteria strains were collected from hot springs in Karakoc-Seferihisar, Gulbahce-Urla and Sifne-Cesme in Izmir, Turkey. The samples were identified both by morphological observations and genetic assay. Filamentous cyanobacteria DNA from the collected samples was isolated and extracted, and were analyzed using,a 16S rRNA cyanobacteria-specific PCR and a denaturing-gradient gel electrophoresis (DGGE). Extraction and purification of phycocyanin was performed in two stages, ammonium sulfate saturation and dialysis, gel filtration and ion exchange chromatography. Conventional separation of Geitlerinema sp., Halospirulina sp. and Phormidium animale was successfully performed yielding C-phycocyanin at 493.760 +/- 3.610, 89.060 +/- 3.209, 32.978 +/- 0.350, 4.046 +/- 0.193, 8.303 +/- 0.511 and 4.196 +/- 0.090 mg/g purity from gel filtration and ion exchange chromatography processes, respectively. The SDS-PAGE demonstrated that the P. animale, Halospirulina sp. and Geitlerinema sp. were purified phycocyanin. Overall, in this study, three cyanobacteria were isolated and cultivated in a laboratory conditions, and then the cyanobacterial cells was extracted and purified thermostable C-phycocyanin obtained, which may be used as raw material in food supplement and pharmaceutical industry.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey-TUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [109T037]; Ege University Science and Technology Centre-EBILTEMEge University [09 BIL 027]en_US
dc.description.sponsorshipThis study was financially supported by The Scientific and Technological Research Council of Turkey-TUBITAK (109T037) project and Ege University Science and Technology Centre-EBILTEM (09 BIL 027) project.en_US
dc.identifier.endpage345en_US
dc.identifier.issn0019-5189
dc.identifier.issn0975-1009
dc.identifier.issn0019-5189en_US
dc.identifier.issn0975-1009en_US
dc.identifier.issue5en_US
dc.identifier.startpage338en_US
dc.identifier.urihttps://hdl.handle.net/11454/29014
dc.identifier.volume57en_US
dc.identifier.wosWOS:000469889600005en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherNatl Inst Science Communication-Niscairen_US
dc.relation.ispartofIndian Journal of Experimental Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectDNA isolationen_US
dc.subjectGeitlerinema sp.en_US
dc.subjectHalospirulina sp.en_US
dc.subjectPhormidium animateen_US
dc.subjectPhycobiliproteinsen_US
dc.titlePurification of phycocyanin from isolated and identified hot spring cyanobacteriaen_US
dc.typeArticleen_US

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