A comparative study on extraction processes of Stevia rebaudiana leaves with emphasis on antioxidant, cytotoxic and nitric oxide inhibition activities

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dc.contributor.authorYildiz-Ozturk, Ece
dc.contributor.authorNalbantsoy, Ayse
dc.contributor.authorTag, Ozgur
dc.contributor.authorYesil-Celiktas, Ozlem
dc.date.accessioned2019-10-27T22:58:52Z
dc.date.available2019-10-27T22:58:52Z
dc.date.issued2015
dc.departmentEge Üniversitesien_US
dc.description.abstractIn this paper, the extraction conditions yielding the highest steviol glycosides from the leaves of Stevia rebaudiana were determined using microwave (MAE) and ultrasonically assisted extraction (UAE) and biological activities of the extracts along with analyses of the residue from the extraction process were investigated. Under optimum conditions, 21.21 mg glycosides/g dried leaf in MAE and 14.90 mg glycosides/g dried leaf in UAE were quantified by HPLC analyses. After extraction, total chlorophyll, carotenoid contents and total dietary fibers were quantified as 15.14 mg/100 g, 2.93 mg/100 g and 6.5% in the raffinate phase. The total phenols were determined as 80.13 and 86.47 mg gallic acid/g extract, whereas the total flavonoids were 111.16 and 126.70 mg quercetin/g extract and DPPH radical scavenging activities were 91.39 and 92.40%, respectively. The extracts exhibited no cytotoxicity against healthy cell line and macrophage RAW 264.7 cells. The IC50 values were 68 mu g/ml and >100 mu g/ml for MAE and UAE. Overall, obtained results suggest that stevia extracts and its residue can be utilized holistically on industrial scale. (C) 2015 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) National Graduate Scholarship ProgramTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [2210-C]en_US
dc.description.sponsorshipThis study was supported by a grant from the Scientific and Technological Research Council of Turkey (TUBITAK) 2210-C National Graduate Scholarship Program. The authors are thankful to the Novel Fluidic Technologies and Applications Laboratory and the Natural Product Chemistry Laboratory at Bioengineering Department for access to the facilities. We would also like to extend our thanks to AREL (Research and Education Laboratory, Ege University School of Medicine) for laboratory facilities.en_US
dc.identifier.doi10.1016/j.indcrop.2015.10.010
dc.identifier.endpage971en_US
dc.identifier.issn0926-6690
dc.identifier.issn1872-633X
dc.identifier.issn0926-6690en_US
dc.identifier.issn1872-633Xen_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage961en_US
dc.identifier.urihttps://doi.org/10.1016/j.indcrop.2015.10.010
dc.identifier.urihttps://hdl.handle.net/11454/51662
dc.identifier.volume77en_US
dc.identifier.wosWOS:000366065200114en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofIndustrial Crops and Productsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectStevia rebaudianaen_US
dc.subjectMicrowave assisted extractionen_US
dc.subjectUltrasonically assisted extractionen_US
dc.subjectSteviol glycosidesen_US
dc.subjectBiological activityen_US
dc.titleA comparative study on extraction processes of Stevia rebaudiana leaves with emphasis on antioxidant, cytotoxic and nitric oxide inhibition activitiesen_US
dc.typeArticleen_US

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