Novel fluorescence assay using µ-wells coated by BODIPY dye as an enzymatic sensing platform

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dc.contributor.authorUnal B.
dc.contributor.authorAkarsu M.
dc.contributor.authorKilincarslan R.
dc.contributor.authorDemirkol D.O.
dc.contributor.authorTimur S.
dc.contributor.authorCetinkaya B.
dc.date.accessioned2019-10-27T08:01:08Z
dc.date.available2019-10-27T08:01:08Z
dc.date.issued2019
dc.departmentEge Üniversitesien_US
dc.description.abstractBODIPY dyes are strongly fluorescent small molecules widely used in fluorescence sensing various analytes. In this study, 8-chloromethyl-2,6-diethyl-4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene (BODIPY-CH2Cl) was successfully synthesized and used as a sensing platform in a 96-well microplate. After synthesis, commercial 96-well microplate surfaces covered with Poly-D-Lysine layer were modified with BODIPY. Glucose oxidase (GOx) was selected as a model enzyme to test the applicability of the BODIPY-coated surface as a fluorescence sensing platform. Since oxygen acts as a quencher for BODIPY, the addition of substrate to modified surfaces caused oxygen depletion and resulted in a quick increase fluorescence intensity. The increments were in proportion to the glucose concentration. Using these data, a linear graph was obtained between 0.0025 and 0.1 mM glucose with a LOD of 2.19 µM. There was no interference effect of some compounds, such as uric acid, ascorbic acid, phenols, and ethanol on sensing. Glucose detection in various complex matrices was carried out. © 2018 Elsevier Ltden_US
dc.description.sponsorship2016FEBE013en_US
dc.description.sponsorshipProf. Dr. B. Cetinkaya and Dr. D. Odaci Demirkol thank The Turkish Academy of Sciences ( TUBA , Turkey) for support. Pamukkale University (project no. 2016FEBE013) is gratefully acknowledged for financial support. Appendix A --en_US
dc.identifier.doi10.1016/j.measurement.2018.11.020
dc.identifier.endpage150en_US
dc.identifier.issn0263-2241
dc.identifier.issn0263-2241en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage145en_US
dc.identifier.urihttps://doi.org/10.1016/j.measurement.2018.11.020
dc.identifier.urihttps://hdl.handle.net/11454/24918
dc.identifier.volume135en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevier B.V.en_US
dc.relation.ispartofMeasurement: Journal of the International Measurement Confederationen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBiofunctional surfaceen_US
dc.subjectBODIPYen_US
dc.subjectEnzymatic sensingen_US
dc.subjectFluorescence sensorsen_US
dc.subjectµ-Well assaysen_US
dc.titleNovel fluorescence assay using µ-wells coated by BODIPY dye as an enzymatic sensing platformen_US
dc.typeArticleen_US

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