Endothelin Receptor-Independent Correlation Between HSP47 and Collagen in Rabbit Model of Early Atherosclerosis

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dc.contributor.authorReel, Buket
dc.contributor.authorCavdar, Zahide
dc.contributor.authorErgur, Bekir Ugur
dc.contributor.authorOzkal, Sermin
dc.contributor.authorOzsarlak-Sozer, Gonen
dc.contributor.authorOktay, Gulgun
dc.contributor.authorKerry, Zeliha
dc.date.accessioned2019-10-27T21:35:11Z
dc.date.available2019-10-27T21:35:11Z
dc.date.issued2011
dc.departmentEge Üniversitesien_US
dc.description.abstractObjective: Collagen in the extracellular matrix (ECM) plays an important role in modulation of response to the vascular injury during the progression of atherosclerosis and restenosis. Collagen can regulate smooth muscle cell (SMC) proliferation, migration and matrix metalloproteinase (MMP) production. Therefore, collagen turnover in the arteries is an important determinant of intimal thickening. Heat shock protein 47 (HSP47), a collagen-specific molecular chaperone, is thought to be essential for the processing and secretion of procollagen molecules. Endothelin (ET) is a strong chemoatractant and mitogen promoting SMC proliferation and migration. The aim of this study was to investigate the possible role of HSP47 and its relation to collagen synthesis, and the effects of a nonselective ETA/ETB receptor antagonist, TAK-044 in collar-induced early atherosclerosis model. Material and Methods: New Zealand white rabbits were divided into two groups. Both groups received vehicle (0.9% NaCl 0.8 ml/kg/day, s.c.) or TAK-044 (5 mg/kg/day, s.c.) for three weeks. On 8th day, a non-occlusive silicon collar was placed around the left carotid artery. The right carotid artery was sham-operated. HSP47 expression in carotid arteries were determined immunohistochemically. Furthermore, total collagen levels, collagen expression and type I procollagen expression were established. Results: HSP47 expression correlated with collagen expression did not change in collared arteries. TAK-044 treatment did not affect HSP47 and collagen levels. Conclusion: There was a correlation between HSP47 expression and collagen expression in carotid arteries. However, intimal thickening did not induce HSP47 expression and early collagen development. The ineffectivenes of TAK-044 suggests that ET-1 signaling may not be implicated in HSP47 and collagen in this model.en_US
dc.description.sponsorshipEge UniversityEge University [04ECZ 020]en_US
dc.description.sponsorshipThis study was supported by Ege University Research Fund (Project No: 04ECZ 020 to Z.K.).en_US
dc.identifier.doi10.5336/medsci.2010-19004
dc.identifier.endpage1339en_US
dc.identifier.issn1300-0292
dc.identifier.issn2146-9040
dc.identifier.issue6en_US
dc.identifier.startpage1330en_US
dc.identifier.urihttps://doi.org/10.5336/medsci.2010-19004
dc.identifier.urihttps://hdl.handle.net/11454/45797
dc.identifier.volume31en_US
dc.identifier.wosWOS:000298720200003en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherOrtadogu Ad Pres & Publ Coen_US
dc.relation.ispartofTurkiye Klinikleri Tip Bilimleri Dergisien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectHSP47 heat-shock proteinsen_US
dc.subjectatherosclerosisen_US
dc.subjectcollagenen_US
dc.subjectendothelin-1en_US
dc.subjectrabbitsen_US
dc.titleEndothelin Receptor-Independent Correlation Between HSP47 and Collagen in Rabbit Model of Early Atherosclerosisen_US
dc.typeArticleen_US

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