Bienzymatic fluorescence detection based on paraoxonase and laccase on anthracene-sequestered polyamic acid films: A novel approach for inhibition-based sensors

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dc.contributor.authorEsen, Elif
dc.contributor.authorYazgan, Idris
dc.contributor.authorDemirkol, Dilek Odaci
dc.date.accessioned2021-05-03T20:36:45Z
dc.date.available2021-05-03T20:36:45Z
dc.date.issued2020
dc.departmentEge Üniversitesien_US
dc.description.abstractA fluorescence assay based on paraoxonase (PO) and laccase (Lac) immobilized on anthracene-sequestered polyamic acid films is now being reported for the first time for ciprofloxacin (CFx) detection. in enzymatic reaction, PO converts phenyl acetate (PA) to acetic acid and phenol. The formed phenol is further oxidized by laccase using oxygen as a co-substrate. For indirectly fluorescence measurements of PA hydrolysis, increased fluorescence intensity was measured after oxygen consumption by laccase. This is because oxygen is quencher of anthracene (Ant) incorporated in the sequestered poly(amic) acid (PAA) film. The detection mechanism was based on inhibition of PO activity by CFx. Using PAA provided the advantage of controlling the film thickness. Firstly, morphology of PAA-Ant polymeric film was characterized by scanning electron microcopy (SEM) and the success of PO-Lac immobilization on PAA-Ant was proven by SEM plus Energy-dispersive X-ray spectroscopy (SEM-EDX) and fluorescence measurements. Then Ant-PAA/PO/Lac is calibrated for PA and CFx without any interfering of some potential interferences. All results make it a promising tool for monitoring CFx at minute levels in samples.en_US
dc.description.sponsorshipEge University Research FoundationEge University [2017FEN012]en_US
dc.description.sponsorshipAuthors thank to Ege University Research Foundation (Project no: 2017FEN012) for support.en_US
dc.identifier.doi10.1016/j.mtcomm.2020.101672
dc.identifier.issn2352-4928
dc.identifier.urihttps://doi.org/10.1016/j.mtcomm.2020.101672
dc.identifier.urihttps://hdl.handle.net/11454/70189
dc.identifier.volume25en_US
dc.identifier.wosWOS:000601000200003en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofMaterials Today Communicationsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectParaoxonaseen_US
dc.subjectLaccaseen_US
dc.subjectInhibition-based sensingen_US
dc.subjectAntibiotic detectionen_US
dc.titleBienzymatic fluorescence detection based on paraoxonase and laccase on anthracene-sequestered polyamic acid films: A novel approach for inhibition-based sensorsen_US
dc.typeArticleen_US

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