Chitosan/Nitrogen Doped Reduced Graphene Oxide Modified Biosensor for Impedimetric Detection of microRNA

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dc.contributor.authorEksin, Ece
dc.contributor.authorBikkarolla, Santosh Kumar
dc.contributor.authorErdem, Arzum
dc.contributor.authorPapakonstantinou, Pagona
dc.date.accessioned2019-10-27T10:42:00Z
dc.date.available2019-10-27T10:42:00Z
dc.date.issued2018
dc.departmentEge Üniversitesien_US
dc.description.abstractThe development of a low-cost and disposable biosensor platform for the sensitive and rapid detection of microRNAs (miRNAs) is of great interest for healthcare, pharmaceuticals, and medical science. We designed an impedimetric biosensing platform using Chitosan (CHIT)/nitrogen doped reduced graphene oxide (NRGO) conductive composite to modify the surface of pencil graphite electrodes (PGE) for the sensitive detection of miRNAs. An initial optimisation protocol involved investigation of the effect of NRGO concentration and miR 660DNA probe concentration on the response of the modified electrode. After the optimization protocol, the sequence-selective hybridization between miR 660 DNA probe and its RNA target was evaluated by measuring changes on charge transfer resistance, R-ct values. Moreover, the selectivity of impedimetric biosensor was tested in the presence of non-complementary miRNA (NC) sequences, such as miR 34a and miR16. The hybridization process was examined both in phosphate buffer (PBS) and in PBS diluted fetal bovine serum (FBS:PBS) solutions. The biosensor demonstrated a detection limit of 1.72g/mL in PBS and 1.65g/mL in FBS:PBS diluted solution. Given the easy, quick and disposable attributes, the proposed conductive nanocomposite biosensor platform shows great promise as a low-cost sensor kit for healthcare monitoring, clinical diagnostics, and biomedical devices.en_US
dc.description.sponsorshipUlster University; British Council [216182787]en_US
dc.description.sponsorshipA. E. would like to express her gratitude to the Turkish Academy of Sciences (TUBA) as a Principal member for its partial support. S.K.B. acknowledges Vice Chancelor PhD studentship from the Ulster University. P.P. acknowledges a financial support from British Council (Newtown fund, Ref: 216182787).en_US
dc.identifier.doi10.1002/elan.201700663
dc.identifier.endpage560en_US
dc.identifier.issn1040-0397
dc.identifier.issn1521-4109
dc.identifier.issn1040-0397en_US
dc.identifier.issn1521-4109en_US
dc.identifier.issue3en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage551en_US
dc.identifier.urihttps://doi.org/10.1002/elan.201700663
dc.identifier.urihttps://hdl.handle.net/11454/30584
dc.identifier.volume30en_US
dc.identifier.wosWOS:000427246500019en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherWiley-V C H Verlag Gmbhen_US
dc.relation.ispartofElectroanalysisen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectNitrogen doped reduced graphene oxideen_US
dc.subjectchitosanen_US
dc.subjectmicroRNAen_US
dc.subjectelectrochemical impedance spectroscopyen_US
dc.subjectpencil graphite electrodeen_US
dc.titleChitosan/Nitrogen Doped Reduced Graphene Oxide Modified Biosensor for Impedimetric Detection of microRNAen_US
dc.typeArticleen_US

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