Putative agents of fig mosaic disease in Turkey [Türkiye'de incir mozaik hastali{dotless}gi{dotless}ni{dotless}n olasi{dotless} etmenleri]

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dc.contributor.authorÇaglayan K.
dc.contributor.authorMedina V.
dc.contributor.authorGazel M.
dc.contributor.authorSerçe C.U.
dc.contributor.authorSerrano L.
dc.contributor.authorAchon A.
dc.contributor.authorSoylu S.
dc.contributor.authorÇalişkan O.
dc.contributor.authorGümüş M.
dc.date.accessioned2019-10-27T08:36:17Z
dc.date.available2019-10-27T08:36:17Z
dc.date.issued2009
dc.departmentEge Üniversitesien_US
dc.description.abstractLeaves from 58 fig tree accessions showing different severity of mosaic and necrotic symptoms were collected in 2 different regions of Turkey and analysed by transmission electron microscope (TEM) and RT-PCR to determine putative causal agents of fig mosaic disease (FMD). Ultrastructural studies showed that double membrane-bound bodies (DMBs) were mainly observed in the parenchyma and mesophyll cells of infected leaves of the cultivars Göklop and Morgüz and some seedlings showing only mosaic symptoms. These bodies were surrounded by a fibrillar matrix in most of the infected cells. Long, flexuous rod-shaped virus-like particles (LFPs) were also observed in vascular tissues of the Bursa Siyahi{dotless}, Sari{dotless}lop, Sari{dotless}zeybek, and Yediveren cultivars, as well as seedlings that exhibited mosaic and necrotic symptoms together. Isometric virus-like particles (IVLPs) were mainly observed in mesophyll parenchyma cells of cv. Yeşilgüz, which showed very mild mosaic symptoms. Some phytoplasma-like bodies, as well as mosaic symptoms, were also observed in the phloem tissues of cv. Morgüz, Göklop, and Yediveren. These kinds of particles were not observed in the healthy plant leaves. RT-PCR analyses revealed that a PCR product of 3.0 kbp was obtained from virus-purified RNA of cvs. Bursa Siyahi{dotless}, and Sari{dotless}lop and an unknown cultivar (seedling) using degenerate fig-associated primers. When primers specific for FMD agent in Spain were used, a DNA fragment of 750 bp was amplified from cvs. Göklop and Yeşilgüz. However, no product was obtained by using DC-random primers. © TÜBİTAK.en_US
dc.identifier.doi10.3906/tar-0807-20
dc.identifier.endpage476en_US
dc.identifier.issn1300011X
dc.identifier.issn1300-011Xen_US
dc.identifier.issue5en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage469en_US
dc.identifier.urihttps://doi.org/10.3906/tar-0807-20
dc.identifier.urihttps://hdl.handle.net/11454/27369
dc.identifier.volume33en_US
dc.indekslendigikaynakScopusen_US
dc.language.isotren_US
dc.publisherTurkiye Klinikleri Journal of Medical Sciencesen_US
dc.relation.ispartofTurkish Journal of Agriculture and Forestryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectElectron microscopeen_US
dc.subjectFig mosaic diseaseen_US
dc.subjectPCRen_US
dc.subjectTurkeyen_US
dc.titlePutative agents of fig mosaic disease in Turkey [Türkiye'de incir mozaik hastali{dotless}gi{dotless}ni{dotless}n olasi{dotless} etmenleri]en_US
dc.typeArticleen_US

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