A nucleic acid-based electrochemical biosensor for the detection of influenza B virus from PCR samples using gold nanoparticle-adsorbed disposable graphite electrode and Meldola's blue as an intercalator

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dc.contributor.authorAydinlik, Seyma
dc.contributor.authorOzkan-Ariksoysal, Dilsat
dc.contributor.authorKara, Pinar
dc.contributor.authorSayiner, A. Arzu
dc.contributor.authorOzsoz, Mehmet
dc.date.accessioned2019-10-27T21:37:54Z
dc.date.available2019-10-27T21:37:54Z
dc.date.issued2011
dc.departmentEge Üniversitesien_US
dc.description.abstractIn the presented study, a novel method is introduced that demonstrates the electrochemical detection of influenza B virus based on DNA hybridisation. The detection utilised gold nanoparticles (AuNPs) and Meldola's Blue (MDB), which is utilised as an intercalator label. The developed methodology, combined with a disposable pencil graphite electrode (PGE) and differential pulse voltammetry (DPV), was performed using both synthetic oligonucleotides and polymerase chain reaction (PCR) amplicons. The electrochemical oxidation response of guanine (approximately +0.1 V) and the voltammetric reduction signal of MDB (approximately -0.2 V) were measured before and after hybridisation reactions between a single strand DNA probe and its complementary target strain (synthetic target or denatured PCR samples). Before the immobilisation of the synthetic DNA probe of influenza type B virus, the transducer surface was interacted with AuNPs solution using a simple wet adsorption method. AuNP immobilisation was confirmed with cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) to characterise the recognition surface of the genosensor. After the interaction between the PGE and AuNPs, a thiol-linked DNA probe was immobilised onto the nanoparticle-covered surface. When hybridisation occurred between the probe and its synthetic targets or specific PCR products, the highest MDB signal was observed. The probes were also challenged with equal quantities of non-complementary DNA at the PGE surface for the determination of biosensor selectivity. AuNP-coated electrodes showed high sensitivity and selectivity, specifically in real samples for the detection of the hybridisation reaction. The results obtained in the presented study indicated that the electrode surface area could be enhanced with AuNPs. The detection limit of the genosensor was found to be 54 picomoles for the synthetic target and 3.3 x 10(7) molecules for the real samples (PCR) in 30 mu L of sample volume. Future prospects and analytical performance of the sensor is briefly discussed.en_US
dc.description.sponsorshipTUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [SBAG-107S163]; Pharmaceutical Sciences Research Centre (FABAL) of Ege University, Faculty of PharmacyEge Universityen_US
dc.description.sponsorshipThe authors acknowledge financial support from TUBITAK (Projects SBAG-107S163) and the Pharmaceutical Sciences Research Centre (FABAL) of Ege University, Faculty of Pharmacy.en_US
dc.identifier.doi10.1039/c1ay05146f
dc.identifier.endpage1614en_US
dc.identifier.issn1759-9660
dc.identifier.issn1759-9679
dc.identifier.issn1759-9660en_US
dc.identifier.issn1759-9679en_US
dc.identifier.issue7en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1607en_US
dc.identifier.urihttps://doi.org/10.1039/c1ay05146f
dc.identifier.urihttps://hdl.handle.net/11454/46285
dc.identifier.volume3en_US
dc.identifier.wosWOS:000292979500025en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherRoyal Soc Chemistryen_US
dc.relation.ispartofAnalytical Methodsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.titleA nucleic acid-based electrochemical biosensor for the detection of influenza B virus from PCR samples using gold nanoparticle-adsorbed disposable graphite electrode and Meldola's blue as an intercalatoren_US
dc.typeArticleen_US

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