Synthesis of an amine-functionalized naphthalene-containing conducting polymer as a matrix for biomolecule immobilization

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dc.contributor.authorAzak, Hacer
dc.contributor.authorGuler, Emine
dc.contributor.authorCan, Ummugulsum
dc.contributor.authorDemirkol, Dilek Odaci
dc.contributor.authorYildiz, Huseyin Bekir
dc.contributor.authorTalaz, Oktay
dc.contributor.authorTimur, Suna
dc.date.accessioned2019-10-27T21:42:38Z
dc.date.available2019-10-27T21:42:38Z
dc.date.issued2013
dc.departmentEge Üniversitesien_US
dc.description.abstractN-functionalized dithienopyrroles (DTP-NH2) were synthesized and electropolymerized onto a graphite electrode as a novel conducting polymer matrix for biomolecule immobilization. H-1-NMR and C-13-NMR were utilized to investigate the characteristics of the monomer. After that, glucose oxidase (GOx) was immobilized onto the amino-functionalised matrix by means of glutaraldehyde. The surface morphologies of both DTP-NH2 and DTP-NH2-GOx were visualised by using SEM and fluorescence microscopy. The chronoamperometric signals of the electrochemical DTP-NH2-GOx biosensors were measured by monitoring the O-2 consumption during an enzymatic reaction in the presence of glucose at -0.7 V. After the optimization of the pH and scan number of the polymer deposition in batch mode, the DTP-NH2-GOx biosensor was also tested in Flow Injection Analysis (FIA) mode. The DTP-NH2-GOx biosensors had a very good linearity between 0.05 and 1.0 mM, and between 0.1 and 2.5 mM for glucose in batch and FIA modes, respectively. Finally, it was applied for glucose analysis in real samples where commercial glucose kits were used as the reference method to verify the data obtained with the proposed biosensor.en_US
dc.description.sponsorshipEuropean Union through the COST Action [CM1104]; Scientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [112T622, 111T135]en_US
dc.description.sponsorshipThe authors would like to thank the European Union through the COST Action CM1104 "Reducible Oxide Chemistry, Structure and Functions" and the Scientific and Technological Research Council of Turkey (TUBITAK Grant Numbers 112T622 and 111T135) for the financial support of this research.en_US
dc.identifier.doi10.1039/c3ra42212g
dc.identifier.endpage19590en_US
dc.identifier.issn2046-2069
dc.identifier.issn2046-2069en_US
dc.identifier.issue42en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage19582en_US
dc.identifier.urihttps://doi.org/10.1039/c3ra42212g
dc.identifier.urihttps://hdl.handle.net/11454/46935
dc.identifier.volume3en_US
dc.identifier.wosWOS:000325554400048en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherRoyal Soc Chemistryen_US
dc.relation.ispartofRsc Advancesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.titleSynthesis of an amine-functionalized naphthalene-containing conducting polymer as a matrix for biomolecule immobilizationen_US
dc.typeArticleen_US

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