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Öğe Comparisons of clinical findings and serological data in the diagnosis of canine leishmaniosis(2005) Özensoy Töz S.; Özbel Y.; Ertabaklar H.; Yildizli N.; Korkmaz M.; Alkan M.Z.This study aimed to evaluate clinical signs related with canine leishmaniosis (CanL) and to determine their relation with serological results in the differential diagnosis of this disease in dog populations. A total of 253 dogs from houses and a dog shelter in Kuşadasi, an endemic region for leishmaniosis, were investigated for clinical signs related to CanL. Sera collected from the study group were examined by indirect fluorescence antibody (IFA), whole ELISA and rK39 ELISA tests for the serological diagnosis. Popliteal lymph node aspiration materials were examined for the parasitological diagnosis. Clinical signs were separated into two groups as cutaneous and visceral signs and no clinical signs were observed in 51.7% (131/253) of the dogs, while 48.3% (122/253) of the dogs had at least one sign. A total of 42 dogs [29 (23.7%) in the symptomatic and 13 (9.9%) in the asymptomatic group] were diagnosed as CanL serologically and/or parasitologically. Two strains were isolated and identified as Leishmania infantum MON-1. There was no correlation between IFAT titers and the number of the clinical signs (P > 0.05; R square = 0.002). Weight loss was found to be the most common sign of CanL while epistaxis was the least common but specific sign. The seropositivity ratio of CanL is 16.6% (42/253) among dogs in the region and clinical signs were found to be helpful for suspecting CanL but at least one serological and/or parasitological method should be performed for the accurate and differential diagnosis in the dogs. Our findings also showed that the one-third of the dogs in an endemic area for CanL could be asymptomatic. © TÜBİTAK.Öğe Seroprevalence of canine visceral leishmaniasis in Kuşadasi, Turkey(2005) Özensoy Töz S.; Ertabaklar H.; Özbel Y.; Cüneyt Balcioglu I.; Yildizli N.; Alkan M.Z.Human and canine visceral leishmaniasis cases have been reported from Kuşadasi, a town in western Turkey, since 1993. In orcer to better understand the epidemiology of canine visceral leishmaniasis (CanVL) in the region, we aimed: (i) to determine the prevalence of CanVL in Kuşadasi using a randomly selected dog population and (ii) to measure the effect of case control on the prevalence of the disease. In this study, all 109 dogs kept at the dog shelter of Kuşadasi Municipality (reflecting random selectivity) were screened serologically using IFAT and rK39 ELISA in 1999. Ten dogs (9.1%) were seropositive or borderline in 1 of the 2 tests. Seropositive dogs (8) died spontaneously or were euthanized, while borderline dogs (2) underwent monthly serological examinations. One year later, in a second sampling, a total of 85 dogs were examined (27 previously tested and 58 new). Four dogs 4.7%) showed seropositivity. Seropositive dogs (2) were euthanized while borderline dogs (2) underwent monthly serological examinations. The reduction in the prevalence of the disease (from 9.1% to 4.7%) appears to be the result of culling CanVL positive dogs from the general population. © TÜBİTAK.