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    Demonstration of PTZ-induced convulsive-reducing effect of butamirate citrate
    (Anka Publishers, 2018) Erdogan M.A.; Cinar B.P.; Kılıç K.D.; Çavuşoğlu T.; Yiğittürk G.; Çetin E.Ö.; Balcioglu H.A.; Günenç D.; Uyanikgil Y.; Erbas O.
    Butamirate has the possible effects on epileptic seizures, which is thought to perform central antitussive effect via medulla oblongata and nucleus tractus solitarius. In our study, these effects have been investigated on electrophysiological and clinical basis. 48 Sprague-Dawley rats were divided randomly into two groups for EEG recordings and behavioral assesment then these two groups divided to four groups: 6 for control, 6 for saline injection, 6 for relatively-low dose butamirate (5 mg/kg) and 6 for relatively-high dose butamirate (10 mg/kg) for each. Evaluation of the behavioral analyses after giving 70 mg/kg pentylenetetrazol (PTZ) first myoclonic jerk time and racine convulsion scales were analyzed, then in different rats for EEG recordings 35 mg/kg PTZ were given and spike percentages were evaluated in same doses of butamirate. In both 5 mg/kg and 10 mg/kg butamirate groups the FMJ onset times were statistically higher then the saline group, similarly both 5 and 10 mg/kg butamirate groups RCS scores were significantly lower than the saline group. In terms of spike percentages, 5 and 10 mg/kg butamirate were significantly lower than the saline group. As a result in our study, we showed that 5 and 10 mg/kg doses of butamirate have anticonvulsant effects on PTZ induced rats. © 2018, Anka Publishers. All rights reserved.
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    Microbial community diversity associated with Sarcotragus sp. and Petrosia ficiformis from the Aegean Sea based on 16S rDNA-DGGE fingerprinting
    (Taylor and Francis Ltd., 2015) Yiğittürk G.; Uzel A.
    Abstract: Marine sponges are prolific sources for new bioactive compounds and they may contain microorganisms that can comprise up to 40% of the total sponge biomass. Therefore, it is important to understand the function and the specific interactions of sponge-associated microorganisms. In this study, we assessed the composition of predominant bacterial and fungal communities using DGGE fingerprinting of Sarcotragus sp. and Petrosia ficiformis collected from the Aegean Sea. Total community DNA extracted with the indirect DNA isolation method by using collagenase followed by 16S rDNA and ITS amplification of 16S rDNA and ITS amplicons were subjected to DGGE profiling. A total of 8 and 21 individual DGGE bands from 16S rDNA-V3 and ITS1 fragments were sequenced, respectively, and matched to corresponding bacteria and fungi in the GenBank database. Methylobacterium sp. and Chalastospora gossypii from Sarcotragus sp. and Gibberella intermedia and Fusarium subglutinans from Petrosia ficiformis are reported for the first time from marine sponges. Associated microorganisms of Sarcotragus sp. and Petrosia ficiformis are considered as host-specific, as tested sponges from the same geographical location showed different dominant bacterial and fungal diversities. © 2014, © 2014 Taylor & Francis.
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    Therapeutic Effects of Liraglutide, Oxytocin and Granulocyte Colony-Stimulating Factor in Doxorubicin-Induced Cardiomyopathy Model: An Experimental Animal Study
    (Humana Press Inc., 2019) Taşkıran E.; Erdoğan M.A.; Yiğittürk G.; Erbaş O.
    Doxorubicin-induced (DXR) cardiomyopathy is a serious health issue in oncology patients. Effective treatment of this clinical situation still remains to be discovered. In this experimental animal study, we aimed to define therapeutic effects of liraglutide, oxytocin and granulocyte colony-stimulating factor in DXR-induced cardiomyopathy model. 40 male Sprague–Dawley rats were included to study. 32 rats were given doxorubicin (DXR) for cardiomyopathy model. DXR was administered intraperitonally (i.p.) at every other day of 2.5 mg/kg/day at six times. Eight rats were taken as normal group and no treatment was performed. 32 rats given doxorubicin were divided into 4 groups. Group 1 rats were assigned to a placebo group and was given with a 0.9% NaCl saline solution at a dose of 1 ml/kg/day i.p. (DXR + saline), Group 2 rats were given with 1.8 mg/kg/day of Liraglutide i.p. (DXR + LIR), Group 3 rats were given with 160 µg/kg/day oxytocin i.p. (DXR + OX), Group 4 rats were given with 100 µg/kg/day filgrastim i.p. (DXR + G-CSF). All medications were given for 15 days. On day 16, under anesthesia, ECG was recorded from derivation I. After that, blood samples were taken by tail vein puncture for biochemical analysis. Finally, the animals were euthanized and the heart removed and prepared for immunohistochemical examination. All three treatments were shown to ameliorate the toxic effect of doxorubicin in cardiac tissue with the best results in DXR + OX group. DXR + OX group had the most preserved tissue integrity examined by light microscopy, least immune expression level of CASPASE-3 (5.3 ± 0.9) (p < 0.001) the highest ECG QRS wave voltage amplitude (0.21 ± 0.008 mV) (p < 0.00001) least plasma MDA (115.3 ± 19.8 nm) (p < 0.001), TNF-alpha (26.6 ± 3.05 pg/ml) (p < 0.001), pentraxin-3 (2.7 ± 0.9 ng/ml) (p < 0.001), Troponin T (1.4 ± 0.08 pg/ml) (p < 0.001), pro-BNP (11.1 ± 3.6 pg/ml) (p < 0.001) levels among all three treatment groups. Consistent with previous literature, we found that OX treatment decreased oxidative, apoptotic and inflammatory activity in DXR-induced cardiomyopathy rat model as well as provided better tissue integrity and better results in clinically relevant measures of ECG assessment, plasma Troponin T and pro-BNP levels. LIR and G-CSF treatment caused similar results with less powerful effects. Our findings suggest that with the best results in OX treatment group, all three agents including LIR and G-CSF attenuates DXR-induced cardiomyopathy in this rat model. © 2019, Springer Science+Business Media, LLC, part of Springer Nature.