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    Carbon dots and curcumin-loaded CD44-Targeted liposomes for imaging and tracking cancer chemotherapy: A multi-purpose tool for theranostics
    (Elsevier, 2021) Demir, Bilal; Moulahoum, Hichem; Ghorbanizamani, Faezeh; Barlas, F. Baris; Yesiltepe, Ozan; Gumus, Z. Pinar; Timur, Suna
    Growing needs in biomedical applications have driven the development of multifunctional nanoparticles towards theranostically-engineered multimodal platforms. Many strategies for point-effective cellular uptake of theranostics have been described. in this report, multifunctional nanostructures were designed and synthesized as a novel theranostic platform. Liposomes were loaded with curcumin as a therapeutic agent and carbon dots (CDs) as a contrast molecule. Anti-CD44 antibodies were bioconjugated on the liposomal surface to obtain an active targeting nanocarrier. After synthesis, the relative characterization of the vesicles was performed using DLS and TEM techniques. in vitro analysis was conducted to investigate the multimodal properties of the theranostic via testing its toxicity, cell uptake, bioimaging, and chemo/radiotherapy applications on two different cell lines. 3D holographic microscopy was employed to track the treatment effects during experimentation. Results indicated that these particles are efficient tools for clinical applications due to their fluorescent characteristic and increased bioperformance.
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    Preparation of glutathione loaded nanoemulsions and testing of hepatoprotective activity on THLE-2 cells
    (Scientific Technical Research Council Turkey-Tubitak, 2021) Yesiltepe, Ozan; Celik, Emine Guler; Geyik, Caner; Gumus, Zinar Pinar; Demirkol, Dilek Odaci; Coskunol, Hakan; Timur, Suna
    To improve bioavailability and stability of hydrophobic and hydrophilic compounds, nanoemulsions are good alternatives as delivery systems because of their nontoxic and nonirritant nature. Glutathione (GSH) suffers from low stability in water, where its encapsulation in nanoemulsions is a powerful strategy to its stability in aqueous systems. The aim of this study was to obtain nanoemulsions from the hydrophobic/hydrophilic contents of N. sativa seed oil so as to improve GSH stability along with bioavailability of N. sativa seed oil. Then, the prepared nanoemulsions were tested for in vitro hepatoprotective activity against ethanol toxicity. To the best of our knowledge, there is no study on the test of nanoemulsions by the combination of Nigella sativa seed oils and GSH in hepatoprotective activity. Here, nanoemulsions with different contents were prepared using Nigella sativa seed oils. Content analyses and characterisation studies of prepared nanoemulsions were carried out. In order to investigate the protective effects against to ethanol exposure, THLE-2 cells were pretreated with nanoemulsions for 2 h with the maximum benign dose (0.5 mg/mL of nanoemulsions). Ethanol (400 mM) was introduced to pretreated cells and nontreated cells for 48- or 72-h periods, followed by cell viability assay was carried out. Fluorescence microscopy tests revealed the introduction of the nanoemulsions into THLE-2 cells. The findings show that nanoformulations have promising in vitro hepatoprotective effects on the THLE-2 cell line against ethanol exposure.
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    Testing of bioactive-nanovesicles on hepatotoxicity of atypical antipsychotics via digital holography
    (Elsevier Science Bv, 2017) Kirbay, Fatma Ozturk; Geyik, Caner; Guler, Emine; Yesiltepe, Ozan; Gumus, Zinar Pinar; Demirkol, Dilek Odaci; Coskunol, Hakan; Timur, Suna
    Atypical antipsychotic drugs induce hepatic toxicity. Thus, it is of importance to eliminate the side effects of these drugs. Herein we describe the preparation of nanoemulsions with a dietary supplement; wheat germ oil (WGO), to ameliorate the liver damage induced by clozapine and olanzapine. THLE-2 cell line was used as a model to investigate the effects of these nanoemulsions on cell viability as well as antioxidative efficiency after antipsychotic insult. In this context, a conventional cell culture method; MTT was used along with a novel cellular imaging technique called digital holography (DH) to evaluate cell viability. Obtained data confirmed that both clozapine and olanzapine induced the liver damage in in vitro model and WGO nanoemulsions were found to be effective on cells and eliminate the cytotoxic effects of these drugs. Briefly, this study has some outputs as follows; it showed that different dietary supplements can be used in such formulations instead of their pristine forms to increase bioavailability. Also, DH was successfully applied for the monitoring of cell viability and it could be a promising approach as the reactive-free cytotoxicity test. (C) 2017 Elsevier B.V. All rights reserved.