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    Pressurized liquid hot water pretreatment and enzymatic hydrolysis of switchgrass aiming at the enhancement of fermentable sugars
    (Taylor & Francis Ltd, 2019) Gunes, Kaniye; Yaglikci, Mine; Celiktas, Melih Soner
    Pressurized Liquid Hot Water (PLHW) process is an essential and new method to remove lignin from biomass for biorefinery applications. in this study, a cascade method was investigated to obtain reducing sugars of switchgrass using optimization of PLHW and enzymatic hydrolysis. Optimization of pressurized liquid hot water and enzymatic hydrolyses were achieved by Box-Behnken design. First, pretreatments including PLHW were carried out step by step, with respect to the independent variables such as temperature (100, 140, 180 degrees C), pressure (100, 150, 200 bar) and time (20, 40, 60 min) at fixed flow rate (2 ml/min). the conditions which give the most optimized reducing sugar were 122.12 degrees C, 100 bar and 50.08 min and optimized fermentable sugar content was measured 107.49 g/l. Then, enzymatic hydrolysis was applied to solid phase with 4.8 pH citrate buffer at 50 degrees C during 24, 48 and 72 h with the help of Novozyme 188 and Celluclast enzymes. the reducing sugar amount obtained from switchgrass with enzymatic hydrolysis was measured 248.23 g/L, which means totally 355.72 g/L.
  • Küçük Resim Yok
    Öğe
    Pressurized liquid hot water pretreatment and enzymatic hydrolysis of switchgrass aiming at the enhancement of fermentable sugars
    (Taylor & Francis Ltd, 2022) Gunes, Kaniye; Yaglikci, Mine; Celiktas, Melih Soner
    Pressurized Liquid Hot Water (PLHW) process is an essential and new method to remove lignin from biomass for biorefinery applications. In this study, a cascade method was investigated to obtain reducing sugars of switchgrass using optimization of PLHW and enzymatic hydrolysis. Optimization of pressurized liquid hot water and enzymatic hydrolyses were achieved by Box-Behnken design. First, pretreatments including PLHW were carried out step by step, with respect to the independent variables such as temperature (100, 140, 180 degrees C), pressure (100, 150, 200 bar) and time (20, 40, 60 min) at fixed flow rate (2 ml/min). The conditions which give the most optimized reducing sugar were 122.12 degrees C, 100 bar and 50.08 min and optimized fermentable sugar content was measured 107.49 g/l. Then, enzymatic hydrolysis was applied to solid phase with 4.8 pH citrate buffer at 50 degrees C during 24, 48 and 72 h with the help of Novozyme 188 and Celluclast enzymes. The reducing sugar amount obtained from switchgrass with enzymatic hydrolysis was measured 248.23 g/L, which means totally 355.72 g/L.
  • Küçük Resim Yok
    Öğe
    Subcritical water extraction derived lignin for creation of sustainable reinforced composite materials
    (Elsevier Sci Ltd, 2019) Celiktas, Melih Soner; Yaglikci, Mine; Maleki, Farshid Khosravi
    This study aims to provide an algorithmic solution to create convertible products, which is then used to fabricate composite components. The shortest optimization path is adapted to this specific conversion problem. The first task was consecutive subcritical water processing and enzymatic hydrolysis optimized by response surface methodology, whereas the second task focused on lignin added composite material to fabricate reinforcement composite and reduce resin rate. The maximum fermentable sugar amount was obtained as 310.9 g/kg under the optimized conditions of 150 bar, 150.9 degrees C at a flow rate of 8 ml/min for 45 min. Subsequent to subcritical water extraction, remaining solids were processed with enzymes to breakdown cellulose for conversion into simple sugars that can be fermented into biofuels and chemicals, where the highest sugar concentration of 429.45 g/L was reached. Finally, lignin was mixed with epoxy resin at 3 different ratios to fabricate lignin-based glass fiber reinforced composite material.