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Öğe Effect of azithromycin, as an adjunct to nonsurgical periodontal treatment, on microbiological parameters and gingival crevicular fluid biomarkers in generalized aggressive periodontitis(Wiley, 2012) Emingil, G.; Han, B.; Ozdemir, G.; Tervahartiala, T.; Vural, C.; Atilla, G.; Baylas, H.; Sorsa, T.Emingil G, Han B, ozdemir G, Tervahartiala T, Vural C, Atilla G, Baylas H, Sorsa T. The effect of azithromycin, as an adjunct to nonsurgical periodontal treatment, on microbiological parameters and gingival crevicular fluid biomarkers in generalized aggressive periodontitis. J Periodont Res 2012; 47: 729739. (c) 2012 John Wiley & Sons A/S Background and Objective: To study the effectiveness of azithromycin in combination with nonsurgical periodontal therapy on clinical and microbiological parameters, and on the MMP-8 and TIMP-1 levels in gingival crevicular fluid, over a 6-mo time-period in patients with generalized aggressive periodontitis. Material and Methods: Thirty-two patients with generalized aggressive periodontitis were included in this randomized, double-blind, placebo-controlled, parallel-arm study. They were randomly assigned to azithromycin or placebo groups (500 mg once daily for 3 d). Probing depth, clinical attachment levels, presence of bleeding on probing and plaque were recorded. Gingival crevicular fluid samples were obtained from one single-rooted tooth, while microbiological samples were obtained from two single-rooted teeth, all with a probing depth of = 6 mm. Microbiological parameters were analyzed by quantitative real-time PCR for Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, Fusobacterium nucleatum, Prevotella intermedia and total bacteria. Gingival crevicular fluid biomarkers were determined by immunofluorometric assay and ELISA. Results: All clinical parameters improved, and microbiological parameters and gingival crevicular fluid MMP-8 levels significantly decreased, over the 6-mo period (p < 0.05); both groups demonstrated similar improvements. The azithromycin group presented a higher percentage of deep pockets resolved (probing depth reduction of = 3 mm from baseline) compared with the placebo group at 1 mo (p < 0.05). Conclusion: Adjunctive azithromycin therapy provides no additional benefit over nonsurgical periodontal treatment on clinical parameters, microbiological parameters and gingival crevicular fluid biochemical markers investigated in patients with generalized aggressive periodontitis.Öğe Enhanced biodegradation of crude oil in soil by a developed bacterial consortium and indigenous plant growth promoting bacteria(Wiley, 2020) Diallo, M. M.; Vural, C.; Cay, H.; Ozdemir, G.Aims This study aimed to develop an efficient, cost-effective and eco-friendly bacterial consortium to degrade petroleum sludge. Methods and Results Four bacterial strains belonging to generaAcinetobacterandPseudomonaswere selected to constitute three different consortia based on their initial concentration. the highest degradation rate (78%) of 1% (v/v) crude oil after 4 weeks of incubation was recorded when the concentration of biosurfactant (BS) producing isolate was high. Genes, such asalkB, almA,cyp153,pah-rhdGN, nah, phnACandcat23were detected using the polymerase chain reaction method and their induction levels were optimal at pH 7 center dot 0. A crude oil sludge was artificially constituted, and its bacterial composition was investigated using 16S rRNA gene amplicon sequencing. the results showed that the soil bacterial community was dominated by plant growth-promoting bacteria (PGPB) after crude oil treatment. Conclusions Our findings indicate the decontamination of the crude oil contaminated soil was more effective in the presence of both the constituted consortium and PGPB compared to the presence of PGPB alone. Significance and Impact of the Study This study showed that the PGPB (Taibaiella) present in petroleum uncontaminated soil can promote the soil decontamination. the addition of both efficient hydrocarbon-degrading and BS producing bacteria is also necessary to improve the decontamination.Öğe A NEW DEFECT ON LEATHER: MICROBIAL BIO-FILM(Amer Leather Chemists Assoc, 2010) Cadirci, B. H.; Ozgunay, H.; Vural, C.; Yilmaz, O.Hides and Skins are protein based materials containing high amounts of water, which makes them a nutritionally rich media ideal for bacterial growth. Being also the essential raw materials in leather manufacture, it is vital to carefully protect them against bacterial action especially in the early wet processing stages. It is well known that bacterial action can seriously damage the fiber structure of hides and skins. Hair slip, red discoloration and grain pilling are typical examples of bacterial action can lead to damage with subsequent defects on the tanned leather. The present study identifies a new defect caused by bacteria known as "biofilm" on hides and skins. For investigation of the observed defective areas, two techniques were used: Scanning Electron Microscopy (S EM), and Real-Time Polymerase Chain Reaction (RT-PCR). The latter technique, RT-PCR, is a novel rapid method for unique detection, identification and quantification of microorganisms. The results, obtained by applying these techniques, verified the presence of "microbial biofilm" on the leather.Öğe REAL TIME BACTERIAL MONITORING DURING THE BIODEGRADATION OF SELECTED PAHs IN INDUSTRY-BORNE ACTIVATED SLUDGE(Scibulcom Ltd, 2014) Vural, C.; Karabey, B.; Bayrak, D. E.; Ozdemir, G.Selected polyaromatic hydrocarbons (PAHs) were evaluated for their degradation potentials in aerobic completely stirred tank reactor (CSTR) and bacterial monitoring was performed with using real time polymerase chain reaction (real time PCR). For this, identified bacteria were inoculated to continuous stirred tank reactor (CSTR) for degradation of selected PAHs. During the degradation process, bacterial existences and numbers were monitored with using designed primer-probe sets in real time PCR. As a mean of the ageing period, 55 days, pH was 7.5 and the dissolved oxygen (DO) was 4 mg/l. Mean of the chemical oxygen demand (COD) and mixed liquor suspended solids (MLSS) rates in effluent 282 and 219 mg/l, respectively, and sludge volume was 373 ml/l. All selected isolates have shown stable results. As a mean of 36 days of biodegradation, Acinetobacter sp., Burkholderia cepacia, Chryseobacterium sp. and Pseudomonas fluorescens were calculated as 2.3 x 10(5), 2.9 x 10(5), 1.5 x 10(6) and 3.6 x 10(7), respectively.Öğe Real-Time PCR Detection of Candida Species in Biopsy Samples from Non-Smokers with Oral Dysplasia and Oral Squamous Cell Cancer: A Retrospective Archive Study(Multidisciplinary Digital Publishing Institute (MDPI), 2023) İlhan, B.; Vural, C.; Gürhan, C.; Vural, C.; Veral, A.; Wilder-Smith, P.; Özdemir, G.The impact of Candida sp. in the development of oral cancer remains uncertain and requires sensitive analytical approaches for clarification. Given the invasive capabilities of these microorganisms in penetrating and invading host tissues through hyphal invasion, this study sought to detect the presence of five Candida sp. in oral biopsy tissue samples from non-smoker patients. Samples were obtained from patients at varying stages of oral carcinogenesis, including dysplasia, carcinoma in situ, OSCC, and histologically benign lesions, and analyzed using Real-Time PCR. Oral tissue samples from 80 patients (46 males and 34 females) were included. Significantly higher C. albicans presence was detected in the mild/moderate dysplasia group compared to the healthy (p = 0.001), carcinoma in situ (p = 0.031) and OSCC groups (p = 0.000). Similarly, C. tropicalis carriage was higher in tissues with mild/moderate dysplasia compared to healthy (p = 0.004) and carcinoma in situ (p = 0.019). Our results showed a significant increase in the presence of C. albicans and C. tropicalis within the mild/moderate dysplasia group compared to other cohorts. Coexistence of these two microorganisms was observed, suggesting a potential transition from a commensal state to an opportunistic pathogen, which could be particularly linked to the onset of oral neoplasia. © 2023 by the authors.
