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Öğe Characterization of osteoblasts derived from bone marrow stromal cells in a modified cell culture system(Elsevier Gmbh, Urban & Fischer Verlag, 2006) Deliloglu-Gurhan, S. I.; Vatansever, H. S.; Ozdal-Kurt, F.; Tuglu, I.Bone marrow is a complex tissue composed of hematopoietic and stromal. stem cells with the potential to differentiate into adipogenic, fibroblastic, reticular, osteogenic and chondrogenic lineages. Identification of differentiation markers during transformation of stromal cells into osteoblasts in a time-dependent manner may be informative for cell-based tissue engineering. Therefore, we investigated the effects of osteogenic medium (OM) on the proliferation and differentiation of rat bone marrow stromal. cells (BMSCs). BMSCs from adult mate rat tibia and femur were collected and cultured in alpha-MEM medium with 10% fetal bovine serum, penicillin, streptomycin and gentamycin. After three days of culture, the medium covering the adherent cells in culture was changed to OM containing dexamethasone, Na-beta-glycerophosphate and ascorbic acid. As a control., cell. culture was also continued in the original. medium for the same time period. Differentiated osteoblast cells were collected after 7, 10, 14, 21 and 30 days of culture, fixed with 4% paraformaldehyde and their immunolabelling for osteoblast markers osteonectin (ON) and osteocalcin (OC) was assessed using an indirect immunoperoxidase technique. Immunoabelling of ON and OC was detectable from day 10 of culture, began to increase on day 14, and increased steadily through to day 21. Labelling was highest on day 30 and was more intense in cells cultured with OM compared to the culture without OM. The control cells cultured in the absence of OM produced negligible levels of both markers. In conclusion, our culture system facilitated differentiation of BMSCs into osteoblasts featuring osteoblast markers, and these cells may be useful in autologous bone implant for the treatment of bone wound heating. (C) 2005 Elsevier GmbH. All rights reserved.Öğe Differentation of human spermatogenetic stem cells from azospermia patients to make sperm-like cells(Oxford Univ Press, 2012) Gozuacik, D.; Vatansever, H. S.; Kara, B.; Calimlioglu, N.; Yasar, P.; Tavmergen, E.; Goker, E. Tavmergen; Semerci, B.; Baka, M.; Ozbilgin, K.Öğe The effect of different implant biomaterials on the behavior of canine bone marrow stromal cells during their differentiation into osteoblasts(Taylor & Francis Ltd, 2016) Ozdal-Kurt, F.; Tuglu, I.; Vatansever, H. S.; Tong, S.; Sen, B. H.; Deliloglu-Gurhan, S. I.We investigated the effects of different implant biomaterials on cultured canine bone marrow stromal cells (BMSC) undergoing differentiation into osteoblasts (dBMSC). BMSC were isolated from canine humerus by marrow aspiration, cultured and differentiated on calcium phosphate scaffold (CPS), hydroxyapatite, hydroxyapatite in gel form and titanium mesh. We used the MTT method to determine the effects of osteogenic media on proliferation. The characteristics of dBMSC were assessed using alizarin red (AR), immunocytochemistry and osteoblastic markers including alkaline phosphatase/von Kossa (ALP/VK), osteocalcin (OC) and osteonectin (ON), and ELISA. The morphology of dBMSC on the biomaterials was investigated using inverted phase contrast microscopy and scanning electron microscopy. We detected expression of ALP/VK, AR, OC and ON by day 7 of culture; expression increased from day 14 until day 21. CPS supported the best adhesion, cell spreading, proliferation and differentiation of BMSCs. The effects of the biomaterials depended on their surface properties. Expression of osteoblastic markers showed that canine dBMSCs became functional osteoblasts. Tissue engineered stem cells can be useful clinically for autologous implants for treating bone wounds.Öğe Three-dimensional vascularized self-assembled tumor spheroids(Wiley-Blackwell, 2016) Bayir, E.; Kabadayi, H.; Gorgun, C.; Anil, M.; Iz, S. Gulce; Vatansever, H. S.; Sendemir-Urkmez, A.
