Yazar "Uysal, A." seçeneğine göre listele
Listeleniyor 1 - 20 / 36
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Alterations of brain tissue in fetal rats exposed to nicotine in utero: possible involvement of nitric oxide and catecholamines(Wiley-Blackwell, 2004) Parlar, A.; Onal, A.; Ulker, S.; Uysal, A.; Delen, Y.; Yurtseven, M. E.; Evinc, A.Öğe ATRA ARSENIC COMBINATION TREATMENT EXPERIENCE WITH THE ACUTE PROMYELOCYTIC LEUCEMIA: SINGLE CENTER EXPERINCE(Pergamon-Elsevier Science Ltd, 2016) Tuncer, F.; Uysal, A.; Patir, P.; Koseoglu, F. D.; Donmez, A.; Tombuloglu, M.Öğe ATYPICALLY LOCALIZED HODGKIN'S LYMPHOMA(Pergamon-Elsevier Science Ltd, 2014) Uysal, A.; Comert, M.; Ozsan, N.; Omur, O.; Saydam, G.; Sahin, F.Öğe Cancer stem cell differentiation: TGF beta 1 and versican may trigger molecules for the organization of tumor spheroids(Spandidos Publ Ltd, 2014) Oktem, G.; Sercan, O.; Guven, U.; Uslu, R.; Uysal, A.; Goksel, G.; Ayla, S.; Bilir, A.Cancer stem cells (CSCs) have the ability to self-renew similar to normal stem cells. This process is linked with metastasis and resistance to chemotherapy and radiotherapy. In the present study, we constructed an in vitro differentiation model for CSCs. CSCs isolated and proliferated for one passage were maintained as monolayers or spheroid-forming cells with serum included media for differentiation process. Differentiation of adhesion molecules and cellular ultrastructural properties were investigated and compared in both monolayer and spheroid cultures. CD133(+)/CD44(+) cancer-initiating cells were isolated from DU-145 human prostate cancer cell line monolayer cultures and propagated as tumor spheroids and compared with the remaining heterogeneous cancer cell bulk population. Microarray-based gene expression analysis was applied to determine genes with differential expression and protein expression levels of candidates were analyzed by immunohistochemistry. Electron microscopy showed detailed analysis of morphology. TGF beta 1 was found to be significantly upregulated in monolayer CSCs. High expression levels of VCAN, COL7A1, 1TG beta 3, MMP16, RPL13A, COL4A2 and TIMP1 and low expression levels of THBS1, MMP1 and MMP14 were detected when CSCs were maintained as serum-grown prostate CSC spheroids. Immunohistochemistry supported increased immunoreactivity of TG beta 1 in monolayer cultures and VCAN in spheroids. CSCs were found to possess multipotential differentiation capabilities through upregulation and/or downregulation of their markers. TGF beta 1 is a triggering molecule, it stimulates versican, Co17A1, ITG beta 3 and, most importantly, the upregulation of versican was only detected in CSCs. Our data support a model where CSCs must be engaged by one or more signaling cascades to differentiate and initiate tumor formation. This mechanism occurs with intracellular and extracellular signals and it is possible that CSCc themselves may be a source for extracellular signaling. These molecules functioning in tumor progression and differentiation may help develop targeted therapy.Öğe CARFILZOMIB TREATMENT IN RELAPSED/REFRACTORY MULTIPLE MYELOMA: SINGLE CENTER EXPERIENCE(Pergamon-Elsevier Science Ltd, 2016) Uysal, A.; Ozkan, M. Comert; Soyer, N. Akad; Sahin, F.; Vural, F.; Tobu, M.; Tombuloglu, M.; Saydam, G.Öğe Chemotherapy influences inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS) activity on 3D breast cancer cell line(Cognizant Communication Corp, 2006) Oktem, G.; Bilir, A.; Selvi, N.; Yurtseven, M. E.; Vatansever, S.; Ates, U.; Uysal, A.; Omay, S. B.Multicellular tumor spheroids (NITS) are three-dimensional structural forms of tumors grown in vitro in the laboratory. In this study, the aim was to determine the regulation of inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS) expressions on NITS in response to treatment with the commonly used anti-cancer drugs Doxorubicin and Docetaxel. The spheroids were generated using the "liquid overlay" technique. The distribution of both iNOS and eNOS was detected using indirect immunohistochemistry, while the expression of both iNOS and eNOS was measured using Western blots. Additionally, S-phase analysis using 5-bromo-2'-deoxyuridine (BrdU) was done on the MTS after treatment with doxorubicin, docetaxel, and a combination of the two. The Griess method was used to measure nitric oxide (NO) production in the cells. An increase in iNOS immunoreactivity and a decrease in eNOS immunoreactivity were observed after doxorubicin treatment, when compared with the other groups. Furthermore, upregulation of iNOS and downregulation of eNOS were detected in doxorubicin-treated cells using Western blotting. Insignificant iNOS expression was observed in all of the groups, and it was particularly low in the control and drug combination groups. NO production was also found to be significantly high after docetaxel treatment, and cell proliferation decreased after doxorubicin treatment. In conclusion, chemotherapy influences NOS activity differently with the presence of different drugs. The results with iNOS show that doxorubicin is a more effective drug than docetaxel, and a drug combination may play a helpful role in the suppression of tumorigenicity and cancer metastasis. Interestingly, eNOS expression increased after the addition of both docetaxel and the drug combination, and it was found to negatively correlate with the histological grade of the tumor. Therefore, analyzing the expression of both iNOS and eNOS might be very useful for targeting the treatment of breast carcinoma and obtaining better information on prognosis.Öğe Compairment of autophagy and mTOR pathways in mouse embryonic stem cell, lung cancer and somatic fibroblast cell lines in molecular analysis base.(Amer Soc Cell Biology, 2017) Oltulu, F.; Kocaturk, D. Calik; Adali, Y.; Ozdil, B.; Acikgoz, E.; Gurel, C.; Uysal, A.; Yavasoglu, A.; Oktem, G.; Aktug, H.Öğe The effects of aerobic exercise frequencies on liver fibrosis, alpha-fetoprotein and cytokeratin 19 in experimental type 2 diabetes-induced rats: an immunohistochemistry study(Taylor & Francis Ltd, 2018) Uslu, S.; Alaca, N.; Kilic, K. D.; Uysal, A.; Kurtel, H.We investigated using immunohistochemistry the effects of frequency of aerobic exercise on liver fibrosis and measured the expression of the oval cell marker, alpha fetoprotein (AFP), and the hepatocellular carcinoma marker, CK 19, in rats with early-period induced type 2 diabetes (T2DM). Rats were divided into four groups: control sedentary rats, diabetic sedentary rats, diabetic rats with continuous exercise (30 min/day, 5 days/week) and diabetic rats with short periods of exercise (3 x 10 min/day, 5 days/week). T2DM was induced using an intraperitoneal (i.p.) injection of nicotinamide (NA) and streptozotocin (STZ). Liver samples were obtained 8 weeks after injection. Tissue sections were stained with hematoxylin and eosin, periodic acid-Schiff and Masson's trichrome. We also used immunochemical staining for AFP, smooth muscle actin (alpha-SMA) and CK19. Continuous and short periods of aerobic exercise produced similar effects during the early period of liver damage in the STZ-NA model, i.e., decreased blood glucose levels and improved body weight, improved liver histology and reduced fibrosis, necrosis and steatosis; and reduced expression of AFP and alpha-SMA. Moderate aerobic exercise for 150 min/week appeared to reduce early liver damage in a rat model of T2DM.Öğe Effects of losartan on experimental varicocele-induced testicular germ cell apoptosis(Wiley-Blackwell, 2016) Bolat, D.; Oltulu, F.; Uysal, A.; Kose, T.; Gunlusoy, B.; Yigitturk, G.; Turk, N. S.; Turan, T.To investigate the potential protective effects of losartan on varicocele-induced germ cell apoptosis, 24 adult male Sprague Dawley rats were divided into three groups: a sham operation was performed in SHAM group, and experimental left varicocele was created in VAR and VAR+LOS groups. Additionally, in VAR+LOS group, losartan was administered for 30days starting on the day of surgery. At the end of 30days, all animals were sacrificed and left orchiectomy was performed. Testicular injury and spermatogenesis were evaluated according to Johnsen scoring system. To assess the nitrosative stress, immunohistochemical staining for endothelial nitric oxide synthase was used and evaluated by H-score and apoptotic index (AI) of germ cells was analysed by TUNEL method. A significant decrease in the mean Johnsen score (JS) was observed in VAR group compared with SHAM (p<.001). The mean H-score and AI were significantly higher in VAR group compared with SHAM (p<.001). After losartan administration, mean JS was significantly increased (p<.001) and mean H-score and AI were significantly decreased compared with VAR group (p<.001 and .01, respectively). Findings of this suggest that losartan acts as a potent protective agent against varicocele-induced germ cell apoptosis.Öğe Effects of sunitinib on immunoreactivity of vimentin, E-cadherin and S100 in kidneys of streptozotocin induced diabetic mice(Taylor & Francis Ltd, 2018) Akarca-Dizakar, S. O.; Aktug, H.; Oltulu, F.; Oktem, G.; Yavasoglu, A.; Acikgoz, E.; Yigitturk, G.; Demir, K.; Uysal, A.Diabetes mellitus (DM) affects many organs including kidney. Tyrosine kinase can cause hypoglycemia and sunitinib is an inhibitor of tyrosine kinase. We investigated the possible effects of sunitinib on the kidney of streptozotocin (STZ) induced type 1 diabetic mice. We used 28 CD 1 type male mice divided into four groups of seven. Type 1 diabetes was induced by injection of STZ. Group 1 was the untreated control. Group 2 comprised non-diabetic mice + sunitinib. Both groups 1 and 2 exhibited normal blood glucose levels. Group 3 comprised STZ treated diabetic mice + saline. Group 4 were diabetic mice + sunitinib treatment. Kidneys were removed after 8weeks. The immunoreactivities of vimentin, E-cadherin and S100 were assessed. Immunostaining of vimentin, E-cadherin and S100 was located in both the glomeruli and tubules of the kidney. We found that the number of vimentin and E-cadherin positive glomeruli and tubules were increased after sunitinib treatment compared to saline treated diabetic mice. The number of vimentin labeled tubules was decreased in the sunitinib treated group compared to diabetic + saline groups. Differences in the number of S100 positive tubules and glomeruli between groups 3 and 4 were not statistically significant. The effect of sunitinib on experimental diabetic mice appears to be related to levels of vimentin, E-cadherin and S100 in the glomeruli and tubules of the kidney, and sunitinib may protect against renal damage from DM.Öğe EFFICACY AND SAFETY OF LENALIDOMIDE AND DEXAMETHASONE IN PATIENTS WITH RELAPSED/REFRACTORY MULTIPLE MYELOMA: A REAL LIFE EXPERIENCE(Pergamon-Elsevier Science Ltd, 2016) Soyer, N.; Patir, P.; Uysal, A.; Duran, M.; Unal, H. D.; Tombuloglu, M.; Sahin, F.; Vural, F.; Saydam, G.Öğe EFFICACY AND SAFETY OF LENALIDOMIDE AND DEXAMETHASONE IN PATIENTS WITH RELAPSED/REFRACTORY MULTIPLE MYELOMA: A REAL LIFE EXPERIENCE FROM TURKEY(Ferrata Storti Foundation, 2017) Saydam, G.; Soyer, N.; Patir, P.; Uysal, A.; Duran, M.; Durusoy, R.; Sahin, F.; Vural, F.; Tobu, M.; Tombuloglu, M.Öğe Evaluation Of Acute Graft Versus Host Disease In Allogeneic Hematopoietic Stem Cell Transplantations(Nature Publishing Group, 2015) Sahin, F.; Comert, M.; Uysal, A.; Tombuloglu, M.; Soyer, N.; Saydam, G.; Vural, F.Öğe Evaluation Of Acute Graft Versus Host Disease In Allogeneic Hematopoietic Stem Cell Transplantations(Nature Publishing Group, 2015) Sahin, F.; Comert, M.; Uysal, A.; Tombuloglu, M.; Soyer, N.; Saydam, G.; Vural, F.Öğe Evaluation Of Acute Graft Versus Host Disease In Allogeneic Hematopoietic Stem Cell Transplantations(Nature Publishing Group, 2015) Sahin, F.; Comert, M.; Uysal, A.; Tombuloglu, M.; Soyer, N.; Saydam, G.; Vural, F.Öğe Evaluation of an elderly care training programme for women(Wiley-Blackwell, 2010) Bayik, T. A.; Uysal, A.Background: Caregiving across different cultures has been perceived conventionally as a private or family responsibility, predominantly performed by women who accept their caregiving as part of their gender role. Aim: This study aimed to design, deliver, and evaluate an elderly training programme for women by assessing their knowledge, attitudes and skills as a lay caregiver. Encouraging the women to find suitable positions for employment in private or governmental institutions was the further objective of the study. Design: The study was a quasi-experimental one-group pre-test post-test design. Methods: The study was conducted in a solidarity centre for women and in a nursing home for the elderly. The sample covered 120 women selected from the community by convenience sampling. Data were gathered through pre- and post-test evaluation and observation forms in 2 May-22 December 2005. The training programme consisted of 230 h of didactic sessions, demonstrations and clinical practices. Findings: The mean change in the participants' knowledge score (pre-test: 41.44 +/- 0.92; post-test: 71.16 +/- 1.34) demonstrated a statistically significant improvement in their knowledge. According to clinical observations, most of them displayed satisfactory caring and communication skills towards the elderly. Virtually all participants reported increased skill, knowledge and confidence. Conclusion: The developed training programme was effective, resulting in an increased knowledge, the acquisition of good attitudes towards the elderly, and performing satisfactory caring and communication skills. Similar community-based programmes managed by nurses are recommended to support non-professional caregivers. The research is not only an innovative but also a revolutionary model to promote women.Öğe EVALUATION OF THE EFFECT AND SAFETY OF PONATINIB AS THIRD-LINE TREATMENT FOR PATIENTS WITH CHRONIC MYELOID LEUKAEMIA IN CHRONIC PHASE(Pergamon-Elsevier Science Ltd, 2016) Patir, P.; Uysal, A.; Duran, M.; Soyer, N.; Vural, F.; Saydam, G.Öğe Expressional assessment of mouse embryonic stem cell, lung cancer and somatic fibroblast cell lines on the basis of EMT, MAPK and Inflammation.(Amer Soc Cell Biology, 2017) Oltulu, F.; Ozdil, B.; Gurel, C.; Acikgoz, E.; Kocaturk, D. Calik; Adali, Y.; Uysal, A.; Yavasoglu, A.; Oktem, G.; Mukhtarova, G. Gursel; Aktug, H.Öğe Hematopoietic Stem Cell Transplantation for Acute lymphoblastic Leukemia Patients: A Single Center Experience(Nature Publishing Group, 2016) Uysal, A.; Soyer, N.; Patir, P.; Tombuloglu, M.; Saydam, G.; Vural, F.Öğe Hepatic progenitor cell inhibition during embryonic period with high dose verapamil, liable joint to the cancer therapy(Comenius Univ, 2013) Uslu, S.; Uysal, A.; Bilir, A.; Soner, B. C.; Oktem, G.Cancer stem cells (CSCs) have been observed to share certain characteristics with normal stem cells. It was an important argument for cancer therapy and a successful progenitor inhibition could show us targeted cell type for a novel strategy. In this study, we aimed to constitute an inhibition in different stages of hepatic stem/progenitor cells (HPCs) with verapamil. Expression patterns of alpha-fetoprotein (AFP), c-kit (CD117) and p-glycoprotein were investigated in developing mouse on the embryonic day (E) 15, E18 and E21 to characterize early and late stages of HPCs. Proliferation inhibition with 5-Bromo-2-Deoxyuridin (BrdU) incorporation and maturation inhibition with PAS staining results were supported by morphometrical analysis during these periods. AFP, c-kit and p-glycoprotein immunoreactivity increased especially in E15 but decreased in E18 and E21 of the control groups during embryonic development. Verapamil treatment effected particularly E15 cells and immunoexpression of HPCs significantly decreased. Proliferation inhibition was observed in all embryonic days of mouse with verapamil and this drug inhibited not only maturation of HPCs in E18 and E21 embryos, but also decreased HPC number in the same embryonic period. According to our results, we estimated that similar to the early and late progenitor stages of HPCs, CSCc can also be in different stages in a heterogenic tumour bulk and the difficulty of CSC inhibition could be the main mechanism of tumour relapses. In this study, HPCs inhibition by verapamil in E15 was not observed in E18 and E21. As similar, CSCs treatments targeting different stages may be impotent to cells in tumour initiating cell stage. We can speculate that ineffectiveness of CSC-specific therapies may be attributed to the highly selective specificity of the treatment (Fig. 6, Ref. 28). Full Text in PDF www.elis.sk.
