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Öğe The ability of 67Ga scintigraphy to detect the lesions of Echinococcus multilocularis infection: Preliminary results(2006) Inceboz T.; Mavi A.; Çapa Kaya G.; Korkmaz M.; Goktay Y.; Yilmaz O.; Uner A.; Durak H.Aim: To assess the ability of 67Ga scintigraphy to detect the lesions of Echinococcus multilocularis (EM) infection. Materials and Methods: An animal model of EM infection was developed. The infected tissues taken from stock infection were placed into the abdominal cavity of uninfected animals operatively. The success of implantation was controlled 20-25 days after implantation. Five infected and 2 healthy animals were studied. All of the animals were examined by ultrasound before the scintigraphic evaluation. After the injection of 7.4 MBq (200 µCi) 67Ga citrate intravenously, static images from the whole anterior thorax and abdomen were obtained at 24, 48 and 72 hours. Visual and semiquantitative analyses were performed. In semiquantitative analysis, an irregular region of interest was drawn over the thorax as the background, excluding the heart and a second region of interest was drawn over the abdomen, excluding the liver and spleen. Abdomen/background ratios were calculated using the mean counts. Results: In the visual evaluation, it was noticed that there was considerably increased 67Ga uptake in the abdomens of the infected animals. In infected animals, mean abdomen/background ratios at 48 and 72 hours (3.76 ± 1.04, 4.13 ± 0.72, respectively) were increased compared with mean abdomen/background ratios at 24 hours (2.94 ± 0.77). These increases in abdomen/background ratios were statistically significant at 72 hours (p = 0.04). Between the infected animals and control group, mean abdomen/background ratios were compared, and statistically significant differences were found in the images obtained at 48 and 72 hours. Conclusion: Imaging at 72 hours seems to be more suitable imaging time for the diagnosis of alveolar echinococcosis. 67Ga scintigraphy may successfully demonstrate the lesions of EM infection localized intraperitoneally. The method of 67Ga scintigraphy is useful because it is simple, non-invasive and relatively safe.Öğe A case of myiasis in a patient with psoriasis from Turkey(2008) Dagci H.; Zeyrek F.; Gerzile Y.K.; Sahin S.B.; Yagci S.; Uner A.Myiasis infestations caused by the larvae of flies mostly belonging to Cyclorapha suborders are frequently encountered in Turkey, which is located in the subtropical zone. The skin is a common site for myiasis, and the infestations are likely to develop in infected tissues and poorly attended wounds of the skin. The case, a 30-year-old male patient, was diagnosed with psoriasis 18 years ago. He had psoriatic scales on his right big toe and was receiving corticosteroid and immunosuppressive drugs. A total of 11 fly larvae were removed from the infected right first toe of the patient. Structures of the stigmas seen in the cross-sections taken from the final segments of these larvae were examined and determined as Sarcophaga spp. larvae. Patients with infected tissues should be extremely cautious about their wound hygiene and take required fly control measures especially during summer as flies can find a suitable environment for sustenance. © 2008 Elsevier Ireland Ltd. All rights reserved.Öğe Circulating antigen detection in patient serum for diagnosis of cystic echinococcosis(2012) Tappeh Kh.H.; Uner A.Hydatidosis is a major public health problem with a worldwide distribution in humans. The purpose of this study is to investigate the circulating antigen in the sera of CE patients. This study was performed on 188 sera which were taken from 181 patients who attended the various departments of Ege University of Medical Faculty and to the laboratory of Parasitology department with the suspicion of Cystic Echinococcosis (CE). Sera were tested with IHA and ELISA for Circulating antibodies and with Circulating Antigen-ELISA (CAg-ELISA) test for circulating antigen. All the patients were questioned personally and clinical data were obtained from the departments where they operated to confirm the diagnosis of CE and 53 serum specimens were shown to be taken from CE proven cases. Hydatid Cyst Fluid (HCF) harvested from the fertile cysts in the liver of the infected sheep was used both in preparing the ELISA and IHA tests for detecting antibodies and for immunizing the rabbits to obtain immune serum. Specific polyvalent immunoglobulin's required for CAg-ELISA test in detecting the circulating antigen for CE in sera, were obtained from the rabbits which were immunized against hydatid cyst antigen and used after being purified with ammonium sulphat (NH4)2 So4 precipitation and gel filtration colon chromatography performed in the molecular biology department. In 82 specimens with ELISA and in 81 specimens with IHA presence of antibodies, over the cut-off values, in varying titrations were shown out of 188 specimens. In 2(1.1%) patients who were found to negative by both IHA and ELISA, presence of CE was shown after the surgical operation. The specifity (79.26%) and sensitivity (88.68%) of IHA and specifity (78.52%) and sensitivity (90.57%) of ELISA tests were found. In 24(45.6%) specimen with CAg-ELISA test circulating antigen were detected in values over the cut-off level. All patients with positive CAg-ELISA are also confirmed with the personal data obtained from either personally or from the clinics where they were operated. These results indicated that it will be great help to assess antigen detecting tests in additional to antibody detecting tests in the serodiagnosis of patients with suspicion of CE.Öğe [Demonstration of Cryptosporidium parvum in immune suppressed rats using nested PCR] [İmmun Sistemi Baskılanmış Sıçanlarda Cryptosporidium parvum'un Nested PZR Yöntemi ile Gösterilmesi](2013) Can H.; Caner A.; Döşkaya M.; Değirmenci A.; Karaçali S.; Gürüz Y.; Uner A.[No abstract available]Öğe Detection of Pneumocystis in the nasal swabs of immune-suppressed rats by use of PCR and microscopy.(2013) Can H.; Caner A.; Döşkaya M.; Degirmenci A.; Karaçali S.; Polat C.; Gürüz Y.; Uner A.Detection of Pneumocystis jiroveci colonization in lungs or oral samples due to high sensitivity of PCR methods results in undue treatment of patients without any symptoms of Pneumocystis pneumonia. The aim of the present study is to demonstrate Pneumocystis carinii in rats, immune suppressed by oral and subcutaneous administration of dexamethasone. Blood, oral, nasal and eye swabs were collected prior to immune suppression and 2, 6, 12 weeks after administration of dexamethasone. Also, samples were collected from lung, heart, liver, kidney, diaphragm, brain, spleen, tongue, muscle, eye, intestine, and feces. Cysts and trophozoites were investigated in stained slides and MSG gene was detected by PCR. The results showed that weight loss is significantly higher in rats administered oral dexamethasone (P<0.05). Microscopy was positive only in lungs of rats orally administered dexamethasone. PCR was positive in lungs and oral swabs of rats prior to the administration of dexamethasone. After the administration of dexamethasone, the MSG gene was detected in oral swabs, lungs, spleen, kidney and (for the first time) in nasal swabs. PCR was positive in nasal swabs during the second and sixth weeks of oral and subcutaneous administration of dexamethasone, respectively. Presence of P. jiroveci in nasopharyngeal aspirate, oropharyngeal wash, oral swab, induced sputum or BAL, and absence in nasal swab in a patient without symptoms of PCP may support clinician's decision regarding colonization. Overall, detection of P. carinii in nasal swabs of rats by PCR demonstrated that nasal sampling can be used for the diagnosis of Pneumocystis pneumonia.Öğe A human case of cyclosporiasis after traveling in the subtropics [Yurtdişi seyahat hikayesi olan bir cyclosporiasis olgusu.](2006) Turgay N.; Yolasigmaz A.; Uner A.In this study, Cyclospora oocysts were detected in a 64-year old man who complained of persistent diarrhea, abdominal pain, nausea and vomiting after visiting the Greek Islands in a sailing boat. Round oocysts about 8-9 microm in size with wrinkled walls that varied in color from red to pale pink after staining were found in Kinyoun's modified acid fast stained stool samples. Public health offices and laboratories, general practitioners, and medical microbiology labs should be informed that more attention should be paid to cyclosporiasis causing diarrheal illness and which requires specific screening methods with experienced microscopists in laboratories.Öğe Preparation of 131I-Pyrimethamine and evaluation for scintigraphy of experimentally Toxoplasma gondii-infected rats(2013) Inceboz T.; Yurt Lambrecht F.; Surucu E.; Yilmaz O.; Yavasoglu A.; Durkan K.; Baykara B.; Bekis R.; Uner A.We aimed to assess the ability of 131I-Pyrimethamine scintigraphy to detect the lesions of Toxoplasma gondii infection. An experimental model of toxoplasmosis was developed. The presence of toxoplasmosis was confirmed 60 days after implantation. Pyrimethamine was radioiodinated with I-131. The radioligand was validated by the requisite quality control tests to check its radiolabeling efficiency, in vitro stability and radiochemical purity etc. 131I-Pyrimethamine (specific activity: 7.08 MBq/mol) was injected intravenously into the tail vein of the control and infected rats. Static whole body images of the rats were acquired under the gamma camera at 5min, 45min, 2h, 6h, and 24h following the intravenous administration of the radioactivity (3.7 MBq/rat). Then the scintigraphic data were analyzed both visually and semiquantitatively. Regions of interest (ROIs) were drawn over the organs (thyroid, stomach, liver, bladder, and soft tissues) to calculate the ratios of the radiotracer in infected vs. control rats. The mean ratio of radiotracer in infected/control rats in the liver and diaphragm was over 1 at 45min which persisted till 24h. In conclusion, 131I-Pyrimethamine may be useful agent for diagnosis toxoplasmosis especially involving liver and diaphragm, needs further preclinical validation before being extended for use in clinical applications. © 2013 Informa UK, Ltd.
