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    Analysis of expressed genes in normal and tumoral mammary gland tissue of the terrier dog [Terrier köpeklerinin normal ve tümörlü meme bezi dokusunda eksprese edilen genlerin birincil analizi]
    (Veteriner Fakultesi Dergisi, 2018) Ozdemir Ozgenturk N.; Omeroglu Ulu Z.; Ulu S.; Celik M.; Tellioglu B.; Yildirim F.; Akis Akad I.; Gurel A.; Un C.; Oztabak K.O.
    Mammary gland tumor is the most common type of tumor in female dogs. Data on genes that are involved in tumorigenesis and mechanism of tumor development are insufficient. Comparative studies have been conducted in order to see if tumorigenesis studies in the dog could be a model for human mammary gland tumors. In this study, we constructed two different cDNA libraries from mammary tissue, which were collected from a normal mammary tissue of a healthy Terrier dog and a tumoral mammary tissue of a sick dog. A total 2304 colonies which are randomly picked out from the two libraries were sequenced for developing a dog mammary gland ESTs collection. Raw EST data were analyzed with Phred/Phrap programs and readable EST sequences were assembled with the CAP3 program. All of EST sequences were grouped into 45 contig and 2203 singletons. Putative functions of all unique sequences were designated by NCBI BLAST based on gene homology and annotated by BLAST2GO. The results of this study are a very valuable resource for functional genome studies of the dogs. © 2018, Veteriner Fakultesi Dergisi. All rights reserved.
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    Generation and analysis of expressed sequence tags from Olea europaea L.
    (2010) Ozgenturk N.O.; Oru F.; Sezerman U.; Kuçukural A.; Korkut S.V.; Toksoz F.; Un C.
    Olive (Olea europaea L.) is an important source of edible oil which was originated in Near-East region. In this study, two cDNA libraries were constructed from young olive leaves and immature olive fruits for generation of ESTs to discover the novel genes and search the function of unknown genes of olive. The randomly selected 3840 colonies were sequenced for EST collection from both libraries. Readable 2228 sequences for olive leaf and 1506 sequences for olive fruit were assembled into 205 and 69 contigs, respectively, whereas 2478 were singletons. Putative functions of all 2752 differentially expressed unique sequences were designated by gene homology based on BLAST and annotated using BLAST2GO. While 1339 ESTs show no homology to the database, 2024 ESTs have homology (under 80) with hypothetical proteins, putative proteins, expressed proteins, and unknown proteins in NCBI-GenBank. 635 EST's unique genes sequence have been identified by over 80 homology to known function in other species which were not previously described in Olea family. Only 3.1 of total EST's was shown similarity with olive database existing in NCBI. This generated EST's data and consensus sequences were submitted to NCBI as valuable source for functional genome studies of olive. Copyright © 2010 Nehir Ozdemir Ozgenturk et al.
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    Goat agouti gene polymorphism and its association with coat color in indigenous turkish goat breeds
    (2012) Akis I.; Oztabak K.; Gursel F.E.; Un C.
    Agouti gene has an important effect on pigment synthesis in mammals. It encodes Agouti signaling protein, which stimulates the phaeomelanin synthesis resulting red, yellow coat color. In this study 120 animals from three indigenous Turkish goat breeds were genotyped for 423 G>T polymorphism in exon 4 of Agouti gene using PCRRFLP method. Two alleles T and G were observed. GG genotype was absent in all breeds. No clear association could be found between coat color and alleles of 423 G>T polymorphism. The genetic diversity for the site analyzed in the study was found to be very low in all breeds. As a conclusion we may say that caprine Agouti gene and its association with coat phenotype should be studied further using new polymorphisms and in a greater number of breeds.
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    Karyotype of Anatolian water buffalo (Bubalus bubalis)
    (Kasetsart University, 2014) Yavasoglu N.U.K.; Koksal C.; Yaman Y.; Un C.
    The water buffalo is an important farm animal raised all over the world but mostly in Asia. They were distributed from southern Asia to Europe during the Pleistocene Epoch. They are classified according to their chromosome number and morphological data either as river type (2n=50) or swamp type (2n=48). Anatolian water buffalo was classified as river type and no further karyotyping specification was made up to now. Previous studies have shown that classification based solely on morphological data is not adequate. Therefore we have applied a procedure in order to identify the chromosome number of the Anatolian water buffalo for the purpose of proving them to be of the river type. Our study has shown that Anatolian water buffalo possess 50 chromosomes which proves their classification as river type buffalos. © 2014, Kasetsart University. All rights reserved.