Yazar "Ucar E." seçeneğine göre listele

Listeleniyor 1 - 2 / 2
  • Küçük Resim Yok
    Öğe
    In Vitro/In Vivo Evaluation of Radiolabeled [99mTc(CO)3]+-Hydroxyurea and Fluorescein Isothiocyanate-Hydroxyurea
    (Mary Ann Liebert Inc., 2016) Yilmaz B.; Teksoz S.; Kilcar A.Y.; Ucar E.; Ichedef C.; Medine E.I.; Ari K.
    The aim of current study is to examine hydroxyurea (HU), which is an antineoplastic drug used for the treatment of leukemia, sickle-cell disease, HIV, psoriasis, thrombocythemia, and various neoplastic diseases in two aspects. The active ingredient hydroxyurea was obtained by purification of the capsule form drug, commercially named as HYDREA. Then, [99mTc(CO)3]+core radiolabeling with HU was performed as first aspect. Quality control studies of 99mTc(CO)3-HU complex were performed by thin-layer radiochromatography and high-performance liquid radiochromatography methods. The results demonstrated that the radiolabeling yield was quite high (98.43% ± 2.29%). Also, 99mTc(CO)3-HU complex has good stability during the 24-hour period. Biological behavior of 99mTc(CO)3-HU complex is evaluated by biodistribution studies on Wistar Albino rats. Fluorescein isothiocyanate (FITC) labeling of HU was performed as second aspect. Fluorometric evaluation of binding efficacy and fluorescence imaging studies on MCF7 and Hela cell lines were carried out. It was thought that the knowledge achieved in this study would contribute to using 99mTc(CO)3-HU complex as an imaging agent, which inhibits the DNA synthesis selectively, by inhibiting ribonucleotide reductase enzyme. It was observed that FITC-HU has noteworthy incorporation on both cell lines. © Copyright 2016, Mary Ann Liebert, Inc. 2016.
  • Küçük Resim Yok
    Öğe
    Interaction between green tea extract and 99mTc-pertechnetate on in vivo distribution
    (Kluwer Academic Publishers, 2014) Sabuncu B.; Biber Muftuler F.Z.; Yurt Kilcar A.; Cekic B.; Ucar E.; Unak P.
    People drink various types of tea without knowing the side effects of biological and chemical contents and radiopharmaceutical interactions. In current study, it is aimed to evaluate the effects of green tea extract in different extraction solvents on the radiolabeling of the blood constituents with 99mTc and on the biodistribution of radiopharmaceutical sodium pertechnetate (Na99mTcO4) in male Wistar Albino rats. The extraction of green tea was performed in different solvents. Biodistribution studies were performed on male rats which were treated via gavage with green tea extract in different extraction solvents or saline (0.9 % NaCl) as a control group for 7 days. The radiolabeling of blood constituents performed incubating with SnCl2 and 99mTc. According to experimental results, radiolabeling blood components with 99mTc were not modified in the usage of the different extraction solvents for green tea extraction, but a significant alteration (P < 0.05) of biodistribution of Na 99mTcO4 was observed after treatment with green tea extract in distilled water. Although there is no considerable effect on radiolabeling of blood components, there is an outstanding change on the biodistribution studies especially with green tea extract in distilled water. The identified change monitored in this study may cause to reduce the risk of misdiagnosis and/or avoid the repetition of the examinations in nuclear medicine. © 2014 Akadémiai Kiadó.