Yazar "Tuney-Kizilkaya, Inci" seçeneğine göre listele

Listeleniyor 1 - 6 / 6
  • Küçük Resim Yok
    Öğe
    Ameliorative effect of Halopteris filicina extracts on growth parameters and genomic DNA template stability of tomato (Solanum lycopersicum) under lead chloride stress
    (Csiro Publishing, 2022) Unal, Dilek; Sevim, Gulcin; Varis, Gokay; Tuney-Kizilkaya, Inci; Unal, Bengu Turkyilmaz; Ozturk, Munir
    Lead is a toxic element that accumulates in agricultural soils through various anthropogenic sources. It inhibits the growth and development of plants and causes mutations in DNA. Macroalgae such as Halopteris filicina contain multifunctional components that may improve plant tolerance to lead stress. In this study, seeds of tomato (Solanum lycopersicum) were subjected to six treatments comprising two levels of lead exposure (60 or 120 mu M PbCl2) with or without H. filicina extract (0.5% in distilled water), a distilled water control, and a positive control (H. filicina extract) for 7 days. Physiological responses were investigated. Seedlings that had been treated with 60 and 120 mu M PbCl2 without H. filicina extract showed root growth reduction of 55% and 68.6%, respectively, relative to the control, whereas for 60 and 120 mu M PbCl2-treated seedlings with H. filicina extract applied, the reductions in root growth were lower, at 27.44% and 50.51%. The seedling viability index was decreased by 68.14% at 120 mu M PbCl2 application without H. filicina extract, whereas a 42.48% reduction was recorded for 120 mu M PbCl2-treated seedlings with H. filicina extract applied. Moreover, PbCl2 accumulation resulted in a decrease in leaf pigment content. Leaf pigment content was high in plants receiving the H. filicina extract. The rate of lipid peroxidation caused by PbCl2 was reduced with application of H. filicina extract. Genomic template stability was determined by using the inter simple sequence repeat-PCR technique, which revealed a decrease in DNA stabilisation with an increase in lead accumulation. However, this was alleviated by application of H. filicina extract. Our findings indicate that H. filicina extract both stimulates plant growth and protects from toxic effects by reducing accumulation of metals in the cell.
  • Küçük Resim Yok
    Öğe
    Dose-dependent Plant-promoting Effect of Macroalgae Stypopodium schimperi Extracts in Solanum lycopersicum and Detection of Phloroglucinol Composition
    (Springer Int Publ Ag, 2023) Unal, Dilek; Gurbanov, Rafig; Sevim, Gulcin; Samgane, Gizem; Varis, Gokay; Ozdemir-Kocak, Fadime; Tuney-Kizilkaya, Inci
    This study aimed to investigate using Stypopodium schimperi, brown alga extract, as a biofertilizer. Algae can be an essential bio-stimulant that increases plant growth and development. Brown algae especially have critical biological activities due to their high secondary metabolite content. This study also determined the biochemical and physicochemical composition and phenolic content of the S. schimperi, an alien brown alga in the Mediterranean Sea.Dose-dependent effects of these extracts on seed germination, root-shoot growth, seedling vigor index, and genome stability of Solanum lycopersicum plant were studied. Inductively coupled plasma mass spectrometry (ICP-MS) analysis revealed the primary elemental composition of the effective extract (10%). Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR) spectroscopy showed phloroglucinol composition, the primary structural molecule of a phlorotannin, in the content of the extract. In addition, the phenolic content was determined by the Folin-Ciocalteu method, while the phenol-sulphuric acid method was used to determine carbohydrates by spectrophotometer. Genomic template analysis was conducted by calculating Inter Simple Sequence Repeats (ISSR) profile changes.Na, Mg, K, Fe, Mo, and Se were determined as elemental compositions of the 10% extract according to ICP-MS analysis. The ATR-FTIR resulted in four different spectral bands at 3350 cm(-1), 2936 cm(-1), 1636 cm(-1), and 1414 cm(-1), which were attributed to the phloroglucinol components. Our results showed that the highest phloroglucinol concentration could inhibit root growth and decrease genomic template stability (GST). The GTS difference between the control and 0.5% extract-treated group was approximately 91.38%, as revealed by ISSR analysis. The lowest GTS value (39.66%) was observed in the roots of S. lycopersicum treated with 5% extract.The present study demonstrated for the first time that a low concentration of S. schimperi extract could be used as a biofertilizer in agriculture. An alien macroalga that may be harmful to the ecosystem is being transformed into a beneficial one by being brought into the economy.
  • Küçük Resim Yok
    Öğe
    Identification complexity of critically endangered Squatina squatina (Linnaeus, 1758) and Squatina aculeata Cuvier, 1829 in the Mediterranean Sea (Turkey)
    (Arak Univ, Arak, 2022) Tuney-Kizilkaya, Inci; Bengil, Elizabeth Grace Tunka
    Sharks and rays, besides their economic importance, are ecologically important organisms with a diverse group. After a pregnant Squatina sp. was captured as by-catch, Akyol et al. (2015) published this species as Squatina squatina while the COI and 16S rDNA analysis demonstrated that the species is S. aculeata. This complexity revealed that utilizing morphologic identification solely might not be enough to distinguish these two Squatina species accurately. We aim to compare morphologic and molecular techniques during species identification of critically endangered S. squatina and S. aculeata. Two different gene regions were used for molecular identification of 3 Squatina specimens obtained from the Aegean coasts of Turkey. Sequence analysis of two gene regions was conducted after PCR analysis. An aligned data set was used for creating phylogenetic trees. The results demonstrated that the previously identified S. squatina specimen was revealed as S. aculeata after molecular analysis. Two other specimens which were morphologically identified as S. squatina demonstrated the same results both with molecular and morphological analysis. Our results suggest that adopting morphological identification as the only tool is not enough to accurately determine the Squatina species; both morphological and molecular tools should be used for taxonomical identification of shark species, especially the endangered ones, to assure their conservation status.
  • Küçük Resim Yok
    Öğe
    Lophosiphonia obscura and Polysiphonia sukatarii sp. nov. (Rhodomelaceae, Rhodophyta) from mesohaline Lake Bafa, Turkey
    (Taylor & Francis Ltd, 2022) Diaz-Tapia, Pilar; Tuney-Kizilkaya, Inci; Taskin, Ergun
    Species discovery is facilitated by the application of molecular tools and the exploration of poorly studied habitats. Recent surveys in Bafa Lake, Turkey, which experienced a transition from oligo- to mesohaline conditions during the last 40 years, led to the finding of two species of the genera Lophosiphonia and Polysiphonia. Our molecular and morphological study showed that one of the species corresponded to L. obscura, while the other differs from previously described Polysiphonia species and is proposed as P. sukatarii sp. nov. The new species differs from related congeners by a rbcL sequence divergence >= 5.8% and is morphologically distinguished by having four pericentral cells and trichoblasts arranged several segments apart. Lophosiphonia obscura, and its closely related species L. hemisphaerica comb. nov. and L. boldii comb. nov., have been previously found in similar habitats in Europe and Atlantic North America. Along with its record from Bafa Lake, P. sukatarii sp. nov. has been discovered in open shore algal turfs from the Canary Islands, suggesting that it is a euryhaline species with probably a wide distribution. Our work highlights that poorly studied habitats still harbour undiscovered species and additional surveys are necessary to acquire a more comprehensive understanding of the macroalgal diversity.
  • Küçük Resim Yok
    Öğe
    MOLECULAR AND MORPHOLOGICAL IDENTIFICATION AND DISTRIBUTION OF CYSTOSEIRA C. AGARDH, 1820 SPECIES IN NORTHERN MEDITERRANEAN COASTS OF TURKEY
    (Parlar Scientific Publications (P S P), 2018) Tuney-Kizilkaya, Inci; Sukatar, Atakan
    The brown algae species, Cystoseira spp., forms an important habitat for many marine species. Changes in ecological conditions as salinity, seawater temperature and other factors such as urbanization, costal development, domestic and chemical pollutions effect the distribution and existence of these species. To protect and maintain their existence it is needed to identify and monitor Cystoseira spp. accurately. For this reason the distribution of the Cystoseira species in Northern Aegean Turkish Coasts were compared with previous data. The species were identified by both morphologically and phylogenetically. 35 Cystoseira samples were collected from 20 stations. Morphological features were used to identify 29 samples out of 35. From these species 10 of them gave appropriate results with sequence analysis. The genus Cystoseira has been identified as a potential indicator of water quality in Mediterranean waters, and our preliminary results suggest that Cystoseira species may also be good bioindicators for ecological conditions.
  • Küçük Resim Yok
    Öğe
    Seasonal variations of epiphytic flora, abscisic acid production and physiological response in the brown alga Cystoseira foeniculacea (Linnaeus) Greville
    (Cahiers De Biologie Marine, 2020) Kozak, Aysegul; Unal, Dilek; Tuney-Kizilkaya, Inci
    In seaweeds, growth and reproduction are affected by seasonal and environmental fluctuations during their life cycle and this requires physiological control. in order to provide insights into this questions, the influence of seasonal variations and epiphytic flora on abscisic acid production, proline biosynthesis, phenolic compounds accumulation, and activity of antioxidant enzymes in the brown alga Cystoseira foeniculacea were investigated. in summer, the highest eckol content was found in the upper and lower parts of the thallus. the highest catalase activity was measured during spring, whereas the lowest one was observed in summer. in contrast, the highest ascorbate peroxidase activity was significantly increased during spring and winter in lower parts of the thallus of C. foeniculacea. Similarly, the highest superoxide dismutase activity was measured during winter. Glutathion levels were 2- and 3-fold higher in spring compared to other seasons. the abscisic acid concentration in the lower and upper parts of the thallus showed differences between seasons. Overall, the results suggest that abscisic acid accumulation with catalase activity and proline production could be related to tolerance to increased seawater temperature during summer, while eckol accumulation might be related to epiphytic flora variations.