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Öğe Biocompatible Delivery System for Metformin: Characterization, Radiolabeling and in Vitro Studies(Bentham Science Publ Ltd, 2020) Aydin, Burcu; Ucar, Eser; Tekin, Volkan; Ichedef, Cigdem; Teksoz, SerapBackground: in recent years, the uses of nanotechnology in medicine have an increasing potential as an effective nanocarrier system. These systems are improved with the purpose of maximizing therapeutic activity and minimizing undesirable side-effects. Moreover, radiolabeled nanoparticles can be used as agents for diagnosis and therapeutic purposes in clinical applications. They have three main components: the core, the targeting biomolecule, and the radionuclide. Objective: It is aimed to synthesize Metformin (MET) loaded Solid Lipid Nanoparticles (MET-SLN) and radiolabeled with technetium-99m tricarbonyl core. Methods: the structure of synthesized nanoparticles was characterized by Fourier Transform Infrared Spectroscopy (FTIR). the particle size and morphology of nanoparticles were examined by Dynamic Light Scattering (DLS), and Scanning Electron Microscope (SEM). Quality control studies of radiolabeled MET-SLN [Tc-99m(CO)(3)-MET-SLN] were performed by High-Performance Liquid Radiochromatography (HPLRC) and Thin Layer Radiochromatography (TLRC). Results: the radiolabeling yield of [Tc-99m(CO)(3)-MET-SLN] was found to be 88%. in vitro studies have been performed on cancer lines(MCF7, MDA-MD-231 breast, and HEPG2 liver cancer cells) to determine the biological behavior of Tc-99m(CO)3-MET-SLNs. Conclusion: the results showed that higher uptake values were observed on estrogen-positive MCF7 breast cancer cell line according to estrogen negative MDA-MB-231 breast cancer and HEPG2 liver cancer cell lines.Öğe Bioevaluation of Tc-99m(I) carbonyl-radiolabeled amino acid coated magnetic nanoparticles in vivo(Elsevier Science Sa, 2019) Buyukok, Onur; Ucar, Eser; Ichedef, Cigdem; Cetin, Oguz; Teksoz, SerapIn this paper, amino acid coated and radiolabeled magnetic nanoparticles (MNPs) were prepared as a potential diagnostic agent. Magnetic nanoparticles were synthesized and functionalized by L-alanine and L-cysteine amino acids in one step. Besides surface functionalization, amino acid coating also provided favourable particle size. Structural characterization was performed by scanning electron microscopy. We obtained 30-38 nm and 22-30 nm diameter ranges with spherical shape for L-alanine and L-cysteine coated nanoparticles, separately. Then L-alanine and L-cysteine coated MNPs were radiolabeled with Tc-99m(I)-tricarbonyl core Tc-99m(CO)(3)-Alanine-MNP, Tc-99m(CO)(3)-Cysteine-MNP) with a labeling yield of 63 +/- 3% and 74 +/- 2%. Pure radiolabeled magnetic particles were obtained by washing them with saline solution, and the radiochemical purity of Tc-99m (CO)(3)-Alanine-MNP and Tc-99m(CO)(3)-Cysteine-MNP were found as 94.3 +/- 0.47% and 97.6 +/- 1.06% in each of the final solution, respectively. in addition, both radiolabeled nanoparticles were stabile up to 4 h at 37 degrees C temperature in vitro conditions.Öğe Complexation Behavior of Guanine with Th4+, UO22+, and Ce3+ at Various Temperatures(Amer Chemical Soc, 2010) Teksoz, Serap; Ichedef, Cigdem Acar; Unak, PerihanThe complexation of guanine with Th4+, UO22+, and Ce3+ was investigated using the potentiometric method at a constant ionic strength of I = 0.10 mol.dm(-3) (KCl) in aqueous solutions at elevated temperatures. For each metal ion, stability constants were obtained from the pH-metric data using the program HYPERQUAD2006. The formation of monomeric and polymeric species was taken into consideration for each ion at (25, 37, and 45) degrees C. The thermodynamic parameters for the formation of the guanine complexes were calculated. In conclusion, it is indicated that the values of the stability constants change at various temperatures.Öğe Complexation of gallic acid involving La3+, Sm3+, Th4+ and UO22+ ions in aqueous solution by potentiometry at various temperatures(Springer, 2024) Sismanoglu, Tuba; Ichedef, Cigdem; Akdut, Gizem; Soylu, Guelin Selda Pozan; Medine, Emin Ilker; Teksoz, SerapIn this study, the complexation of gallic acid with various lanthanide and actinide ions (La3+, Sm3+, Th4+, and UO22+) was investigated under aqueous conditions at elevated temperatures by potentiometric titration, with ionic strength of I = 0.10 mol dm-3 KCl. Stability constants for complexation of related metal-ions were determined using HYPERQUAD2013 software. The formation of complexes was considered at temperatures of 25 degrees C, 37 degrees C, and 45 degrees C. By examining the dependence of complex formation on temperature, the thermodynamic parameters of each complex were calculated. It was observed that the stability constant values varied with temperature. Additionally, cytotoxicity studies were conducted on MCF-7 and A-549 cell lines as part of the conclusion.Öğe Design of docetaxel-loaded polymeric nanoparticles: characterization, radiolabeling with Tc-99m and in vitro evaluation(Springer, 2020) Cetin, Oguz; Ichedef, Cigdem; Aydin, Burcu; Teksoz, SerapThis research aims to develop a polymeric drug delivery system for docetaxel (DTX) and to investigate its radiotracer potential by in vitro. DTX loaded Poly(lactic acid)/Poly(ethylene glycol) diblock copolymer (PLA-PEG) was synthesized and radiolabeled with technetium-99m tricarbonyl core. PLA-PEG-DTX nanoparticles had a particle size and zeta potential 120 +/- 7.3 nm and - 24.66 +/- 1.5 mV respectively, which was further supported by SEM image. the radiolabeling efficiency of Tc-99m(CO)(3)-PLA-PEG-DTX was found to be 90 +/- 3.51%,. in vitro study was carried out on cancer cell lines (MCF7, MDA-MB-231) to examine the biological behavior of docetaxel loaded PLA-PEG nanoparticles. [GRAPHICS] .Öğe Design of Tc-99(m) radiolabeled gemcitabine polymeric nanoparticles as drug delivery system and in vivo evaluation(Elsevier Science Sa, 2021) Ichedef, Cigdem; Teksoz, Serap; Cetin, Oguz; Aydin, Burcu; Sarikavak, Ibrahim; Parlak, Yasemin; Bilgin, Bedriye ElvanThe main objective of this study is to create a multifunctional drug carrier system as a prototype product. For this purpose, Gemcitabine that used as an anticancer agent for several kinds of tumour therapy, and technetium(I)-tricarbonyl core, [Tc-99m(CO)(3)](+), was incorporated to the polymeric structure. in the current study PLA-PEG-COOH (polylactic acid-polyethylene glycol-carboxyl) copolymer was synthesized and the structure analysis was performed by FT-IR and H-1 NMR. The surface charge, size and morphology of the polymeric nanoparticles was evaluated by Zeta Potential (ZP) analysis, Dynamic Light Scattering (DLS) and Scanning Electron Microscopy (SEM) methods. Gemcitabine loaded polymeric nanoparticles were radiolabeled with [Tc-99m(CO)(3)(H2O)(3)](+) and quality controls were performed by thin layer radiochromatograpy (TLRC) and high performance liquid radiochmmatograpy (HPLRC). PLA-PEG-GEM complex was labeled with [Tc-99m(CO)(3)](+) in high radiochemical yield and purity. The radiolabeled complex is stable during the study period in saline solution. The biological activity of this radiolabeled system was evaluated performing biodistribution and gamma camera imaging on Wistar Albino rats which exhibited higher uptake in the lung and liver at all selected time points.Öğe Effect of rosemary (Rosmarinus officinalis) extract on the biodistribution of Tc-99m sulphur colloid and on the radiolabeled blood constituents(Soc Brasileira Farmacognosia, 2013) Ucar, Eser; Teksoz, Serap; Ichedef, Cigdem; Kilcar, Ayfer Yurt; Unak, PerihanWith this study we evaluated the effects of the herb rosemary (Rosmarinus officinalis L. Lamiaceae) extract on the labeling of blood constituents with technetium-99m (Tc-99m) labeled sulphur colloid and on the biodistribution of Tc-99m-Sulphur Colloid in Wistar albino rats. For this purpose, two groups of animals (male wistar rats, 130140 g) were treated (1 mL) with a rosemary extract (750 mg/kg body wt., n=9) and water (control, n=9) separately by gavage for five days. Tc-99m-Sulphur Colloid was administrated by intravenous injection; organs/tissues were withdrawn and weighted. Blood was centrifuged, plasma and blood cells were isolated. The radioactivity was counted to calculate the percentage of activity per gram for each organ/tissue and percentage of activity in blood cells and plasma. A significant increase (p<0.05) in the uptake of Tc-99m-Sulphur Colloid in the liver after the treatment with rosemary extract was observed. These results indicate that the substances or metabolites of the rosemary extract would change the biodistribution of Tc-99m-Sulphur Colloid.Öğe Hydrolytic Behavior of La3+ and Sm3+ at Various Temperatures(Springer/Plenum Publishers, 2018) Ichedef, Cigdem; Sismanoglu, Tuba; Teksoz, SerapThe hydrolytic species of lanthanide ions, La3+ and Sm3+, in water at I = 0.1 mol center dot dm(-3) KCl ionic strength and temperatures of 298.15, 310.15 and 318.15 K were investigated by potentiometry. The hydrolytic species were modeled by the HySS simulation program. From the results, the hydrolytic species of each metal ion at different temperatures were calculated using the program HYPERQUAD2013. The hydrolysis constants (log(10) beta) of [La(OH)](2+) and La(OH)(3) were calculated as -8.52 +/- 0.46, -26.84 +/- 0.48, and log(10) beta values of [Sm(OH)](2+), [Sm(OH)(2)](+), Sm(OH)(3) were calculated as -7.11 +/- 0.21, -15.84 +/- 0.25 and -23.44 +/- 0.52 in aqueous media at 298.15 K, respectively. The dependence of the hydrolysis constants on the temperature allowed us to calculate the enthalpy, entropy, and Gibbs energy of hydrolysis values of each species.Öğe In vitro evaluation of radiolabeled methotrexate loaded magnetic nanoparticle delivery system(Springer, 2023) Uzug, Sara; Cetin, Oguz; Aydin, Burcu; Ichedef, Cigdem; Teksoz, SerapMagnetic nanoparticles were synthesized via the chemical co-precipitation method using ammonium hydroxide as a precipitating agent. For this purpose, iron magnetic nanoparticles (Fe3O4) were synthesized, coated with alanine (Ala) and loaded with a folic acid antagonist MTX. Structural characterization of MNP-Ala and MNP-Ala-MTX were achieved by nanoparticle characterization techniques. MTX loaded magnetic nanoparticle was radiolabeled with technetium-99 m with 89% efficiency.The effect of radiolabeled nanoparticles on cells was determined on human cervical cancer (HeLa) and human breast (MCF7) cancer cell lines. It was found that drug loaded MNP nanoparticles showed higher uptake in MCF7 cells than in HeLa cells.Öğe In Vitro/In Vivo Evaluation of Radiolabeled [Tc-99m(CO)(3)](+)-Hydroxyurea and Fluorescein Isothiocyanate-Hydroxyurea(Mary Ann Liebert, Inc, 2016) Yilmaz, Baris; Teksoz, Serap; Kilcar, Ayfer Yurt; Ucar, Eser; Ichedef, Cigdem; Medine, Emin Ilker; Ari, KadirThe aim of current study is to examine hydroxyurea (HU), which is an antineoplastic drug used for the treatment of leukemia, sickle-cell disease, HIV, psoriasis, thrombocythemia, and various neoplastic diseases in two aspects. The active ingredient hydroxyurea was obtained by purification of the capsule form drug, commercially named as HYDREA. Then, [Tc-99m(CO)(3)](+)core radiolabeling with HU was performed as first aspect. Quality control studies of Tc-99m(CO)(3)-HU complex were performed by thin-layer radiochromatography and high-performance liquid radiochromatography methods. The results demonstrated that the radiolabeling yield was quite high (98.43%+/- 2.29%). Also, Tc-99m(CO)(3)-HU complex has good stability during the 24-hour period. Biological behavior of Tc-99m(CO)(3)-HU complex is evaluated by biodistribution studies on Wistar Albino rats. Fluorescein isothiocyanate (FITC) labeling of HU was performed as second aspect. Fluorometric evaluation of binding efficacy and fluorescence imaging studies on MCF7 and Hela cell lines were carried out. It was thought that the knowledge achieved in this study would contribute to using Tc-99m(CO)(3)-HU complex as an imaging agent, which inhibits the DNA synthesis selectively, by inhibiting ribonucleotide reductase enzyme. It was observed that FITC-HU has noteworthy incorporation on both cell lines.Öğe Leucine Complexes with Cerium: Stability Constants and Thermodynamic Parameters(Amer Chemical Soc, 2011) Sismanoglu, Tuba; Teksoz, Serap; Ichedef, CigdemThe complexation of leucine (Leu) with Ce3+ was investigated using the potentiometric method at different ionic strengths (I = (0.10 and 1.00) mol dm(-3)) in KCl medium at elevated temperatures. The stability constants of the leucine complexes with Ce3+ at metal:ligand (M:L) molar ratios of 1:1, 1:3, and 1:5 were determined. From the data obtained, stability constants were calculated using the program HYPERQUAD2006 for proton-ligand and metal-ligand species. The formation of monomeric species was taken into consideration for Ce3+ at (25, 37, and 45)degrees C. Thermodynamic parameters were calculated for all possible leucine complexes. In conclusion, it is indicated that the formation of complexes change the value of the stability constants at various ionic strengths and temperatures.Öğe A new approach to liquid penetrant inspection: radiolabeled QDots(Springer, 2017) Sezgin, Hasan Sezer; Muftuler, Fazilet Zumrut Biber; Ichedef, Cigdem; Kilcar, Ayfer Yurt; Teksoz, Serap; Unak, Perihan; Harmansah, CoskunPenetrant technique is a sensitive non-destructive testing (NDT) method for detecting and locating the presence of cracks in sample surface. Today, NDT is used in a wide range of industries including aerospace, biotechnology, defence, marine, oil-gas and energy plants. This work focuses on potential use of radiolabeled ZnS coated CdTe quantum dots (QDots) as a penetrant for liquid penetrant testing. The synthesized QDots as a precursor were tested for surface defects detection in welded joints. The experimental results show the highest activities were found in defects on the sample surface. These finding are consistent with the sample NDT inspection test report.Öğe Polymer Coated Iron Nanoparticles: Radiolabeling & In vitro Studies(Bentham Science Publ Ltd, 2021) Yilmaz, Selin; Ichedef, Cigdem; Karatay, Kadriye Busra; Teksoz, SerapBackground: Superparamagnetic iron oxide nanoparticles (SPIONs) have been extensively used for targeted drug delivery systems due to their unique magnetic properties. Objective: In this study, it has been aimed to develop a novel targeted Tc-99m radiolabeled polymeric drug delivery system for Gemcitabine (GEM). Methods: Gemcitabine, an anticancer agent, was encapsulated into polymer nanoparticles (PLGA) together with iron oxide nanoparticles via double emulsion technique and then labeled with Tc-99m. SPIONs were synthesized by reduction-coprecipitation method and encapsulated with oleic acid for surface modification. Size distribution and the morphology of the synthesized nanoparticles were characterized by dynamic light scattering (DLS) and scanning electron microscopy (SEM), respectively. The radiolabeling yield of SPION-PLGAGEM nanoparticles was determined via Thin Layer Radio Chromatography (TLRC). Cytotoxicity of GEM loaded SPION-PLGA was investigated on MDA-MB-231 and MCF7 breast cancer cells in vitro. Results: SEM images displayed that the average size of the drug-free nanoparticles was 40 nm and the size of the drug-loaded nanoparticles was 50 nm. The diameter of nanoparticles was deter- mined as 366.6 nm by DLS, while zeta potential was found as 29 mV. SPION was successfully coated with PLGA, which was confirmed by FTIR. GEM encapsulation efficiency of SPION-PLGA was calculated as 4 +/- 0.16% by means of HPLC. Radiolabeling yield of SPION-PLGA-GEM nanoparticles was determined as 97.8 +/- 1.75% via TLRC. Cytotoxicity of GEM loaded SPION-PLGA was investigated on MDA-MB-231 and MCF7 breast cancer cells. SPION-PLGA-GEM showed high uptake on MCF-7, while the incorporation rate was increased for both cell lines with external magnetic field application. Conclusion: Tc-99m labeled SPION-PLGA nanoparticles loaded with GEM may overcome some of the obstacles in anti-cancer drug delivery because of their appropriate size, non-toxic, and super-paramagnetic characteristicsÖğe Preparation and bioevaluation of Tc-99m-carbonyl complex of guanine(Springer, 2011) Ichedef, Cigdem; Teksoz, Serap; Senocak, Kamile; Ucar, Eser; Kilcar, Ayfer YurtThe aim of this study is to prepare radiolabeled guanine with Tc-99m(CO) (3) (+) core. For this purpose, guanine has been radiolabeled with Tc-99m(CO) (3) (+) core. Quality control study of radiolabeled guanine molecule with Tc-99m(CO) (3) (+) core was performed by thin layer radio chromatography (TLRC) and high performance liquid radio chromatography (HPLRC). The results showed that the radiolabeling yield was quite high (94 +/- A 3%). Beside that Tc-99m(CO)(3)-Gua complex has showed good in vitro stability during the 24 h period. Radiopharmaceutical potential of this complex was evaluated in Wistar Albino Rats. It was concluded that Tc-99m(CO)(3)-Gua could be used as a nucleotide radiopharmaceutical for in vivo applications.Öğe A promising radiolabeled drug delivery system for methotrexate: synthesis and in vitro evaluation of Tc-99m labeled drug loaded uniform mesoporous silica nanoparticles(Springer, 2021) Ichedef, Cigdem; Aydin, Burcu; Hamurisci, Selin Irdel; Teksoz, Serap; Medine, Emin IlkerIn present study, we describe a promising radiolabeled drug delivery system for Methotrexate (MTX), an anticancer drug used in the treatment of breast cancer. Uniform and re-dispersible MSN were synthesized with a particle size of as 42.55 +/- 1.45 nm. Then, MTX was loaded into the surface modified MSN with DTPA over 95% loading capacity. Subsequently, MTX loaded MSN carrier system was radiolabeled with Tc-99 m (Tc-99m-MTX-MSN) with 92.20 +/- 0.8% radiolabeling yield. Furthermore, in vitro evaluation on estrogen positive (ER +) MCF7 and estrogen negative (ER-) A549 cells lines were performed for determining apoptotic and cytotoxic effects of MTX-MSN, and incorporation behavior of Tc-99m-MTX-MSN. Drug loaded MSN particles were exhibit higher uptake in MCF7 cells than A549 cells.Öğe Radiolabeled D-Penicillamine Magnetic Nanocarriers for Targeted Purposes(Amer Scientific Publishers, 2016) Ozyuncu, Seniha Yolcular; Teksoz, Serap; Ichedef, Cigdem; Medine, E. Ilker; Avci, Cigir Biray; Gunduz, Cumhur; Unak, PerihanThe aim of this study is to synthesize D-Penicillamine (D-PA) conjugated magnetic nanocarriers for targeted purposes. Magnetic nanoparticles were prepared by partial reduction method and surface modification was done with an amino silane coupling agent's (structural properties), AEAPS, the particles were characterized by Scanning Electron Microscope (SEM), X-ray Diffraction (XRD). After that D-PA was linked with the magnetic nanoparticles (MNPs) and has been radiolabeled with [Tc-99m(CO)(3)](+) core. Quality controls of [Tc-99m(CO)(3)-MNP-D-PA] were established by Cd(Te) detector. The radiolabeling efficiency of magnetic nanoparticles ([Tc-99m(CO)(3)-MNP-D-PA]) was about 97.05% with good in vitro stability during the 24 hour period. As a parallel study, radiolabeled D-PA complex ([Tc-99m(CO)(3)-D-PA]) was prepared with a radiolabeling yield of 97.93%. At the end, biologic activities of binding complexes were investigated on MCF7 human breast cancer cells. Our results show that, radiolabeled magnetic nanoparticles with core[Tc-99m(CO)(3)](+) ([(99)mTc(CO)(3)-MNP-D-PA]) showed the highest uptake on MCF7 cells which were applied magnetic field in the wells. In that case, result of this study emphasizes that radiolabeled magnetic nanoparticles with core [Tc-99m(CO)(3)](+) would support new occurrences of new agents.Öğe Somatostatin with Tc-99m and biodistribution studies in rats(Mary Ann Liebert Inc, 2007) Acar, Cigdem; Teksoz, Serap; Unak, Perihan; Muftuler, F. Zumrut Biber; Medine, E. IlkerSomatostatin (SST) is a short-lived peptide hormone that regulates the endocrine system. The main use of the derivatives of SST is to diagnose diseases related to growth hormone and to use against some forms of cancer that involve growth hormone. Also, SST suppresses gastric acid secretion, gallbladder contractions, and pancreatic enzyme secretion. In this study, two different bifunctional chelating agents were used to examine the changes in the biologic half-life of SST. For this purpose, first D-penicillamine (DPA) and diethylene triaminepentaacetic acid (DTPA) were used to label SST with Tc-99m and then radiopharmaceutical potential of three Tc-99m-labeled complexes, Tc-99m-D-PA, (TC)-T-99m-D-PA-SST, and (99m)TcDTPA-SST, were compared with each other. Quality control for each labeled complex was established by using radiochromatographic methods. The radiolabeled complexes maintained their stabilities for 5 hours. Then, biodistribution studies were performed on Albino Wistar rats independently for three complexes. The results demonstrated that (TC)-T-99m-D-PA-SST exhibited long-term uptake in organs, and its clearance took longer than the Tc-99m-DTPA-SST complex.Öğe Synthesis and Biological Evaluation of Bisphosphonate Compound Labeled with Tc-99m(CO)(3)(+)(Wiley-Blackwell, 2015) Senocak, Kamile; Teksoz, Serap; Ichedef, Cigdem; Ucar, EserIn this study, radiolabeling of a bisphosphonate, alendronate (Alendronate sodium), was performed with the help of a bifunctional chelating agent. For that purpose, DTPA-NHS (Diethylenetriaminepentaacetic acid dianhydride-N-hydroxysuccinimide) was synthesized with an esterification between DTPA and NHS. Combining the DTPA-NHS ester with alendronate yields the DTPA-Alendronate compound. The structure of synthesized compound was analyzed by H-1/C-13/P-31-NMR and HPLC. After then, the labeling with [Tc-99m(CO)(3)](+) core of synthesized compound was provided. Performing quality controls of newly synthesized [Tc-99m(CO)(3)-DTPA-Alendronate] complex with thin layer radiochromatography (TLRC) and high-performance liquid radiochromatography (HPLRC), the labeling yield was found as 99%. It was observed that the compound conserves its stability for 24h in serum media. Biodistribution of the radiolabeled complex was performed on Wistar Albino rats to determine radiopharmaceutical potential of the [Tc-99m(CO)(3)-DTPA-Alendronate] complex. It is thought that the data gained from this study will contribute to the development of complexes with bisphosphonate.Öğe Synthesis, characterization and radiolabeling of folic acid modified nanostructured lipid carriers as a contrast agent and drug delivery system(Pergamon-Elsevier Science Ltd, 2017) Ucar, Eser; Teksoz, Serap; Ichedef, Cigdem; Kilcar, Ayfer Yurt; Medine, E. Ilker; Ari, Kadir; Parlak, Yasemin; Bilgin, B. Elvan Sayit; Unak, PerihanNanostructured lipid carriers (NLCs) are the new generation of solid lipid drug delivery systems. Their suitability as contrast agents for gamma scintigraphy is an attracting major attention. The aim of current study was to prepare surface modified nanostructured lipid carrier system for paclitaxel (PTX) with active targeting and imaging functions. In accordance with the purpose of study, PTX loaded nanostructured lipid carriers (NLCs) prepared, modified with a folate derivative and radiolabeled with technetium-99 m tricarbonyl complex (Tc-99 m(CO)(3)(+)). Cellular incorporation ratios of radiolabeled nanoparticles (Tc-99 m(CO)(3)-PTX-NLC) were investigated in vitro on three cancer cell lines. Additionally in vivo animal studies conducted to evaluate biological behavior of Tc-99 m(CO)(3)-PTX-NLC on female Wistar Albino rats. Biodistribution results showed that the folate derivative modified Tc-99 m(CO)(3)-PTX-NLC had considerably higher uptake in folate receptor positive organs. The data obtained from present study could be useful in the design of biodegradable drug carriers of PTX and folate receptor based tumor imaging agents.Öğe Tc-99m(I) carbonyl-radiolabeled lipid based drug carriers for temozolomide delivery and bioevaluation by in vitro and in vivo(Walter De Gruyter Gmbh, 2019) Ari, Kadir; Ucar, Eser; Ichedef, Cigdem; Kilcar, Ayfer Yurt; Medine, Emin Ilker; Parlak, Yasemin; Teksoz, SerapIn preclinical research radiolabeled nanoparticles have been attracting interest as a new class of imaging probes. Assuming good stability of solid lipid nanoparticles (SLNs) under physiological conditions, radiolabeled SLNs can be used for imaging and measuring uptake in target tissue. Present study was performed to evaluate biological behavior of temozolomide (TMZ) loaded solid lipid nanoparticles (SLN-TMZ) in vivo and in vitro. Lipid nanoparticles were prepared by emulsification and low-temperature solidification method. zeta potential, morphology and particle size of nanoparticles were determined. Biological behavior of( 99m)Tc(CO)(3)(+) radiolabeled SLN-TMZ were investigated in vitro on U87/Daoy cell lines and in vivo on female Wistar Albino rats. Obtained results of in vitro incorporation, in vivo biodistribution and gamma imaging studies on radiolabeled SLN-TMZ show that the radiolabeled solid lipid nanoparticles could have potential as a drug delivery system for TMZ.
