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Öğe Acute hypoxia exposure following prenatal stress impairs hippocampus and novelty-seeking behavior in adolescent rats(Wiley, 2022) Celik, Kubra; Bilim, Petek; Garip, Gurur; Durmaz, Burak; Sozmen, Eser Yildirim; Baka, MeralObjectives The present study aimed to investigate the effects of acute hypoxia exposure following prenatal stress on the novelty-seeking behavior and hippocampus of adolescent rats. Methods The offspring were divided into prenatal stress (PS) and non-stress (NS) groups. Both groups were exposed to hypoxia on postnatal day 10 (P10) while control groups were undisturbed. Novel object recognition task was performed in each group. Next, brains were collected to examine hippocampus via immunohistochemical and biochemical studies on postnatal day 35 (P35). Results PS decreased novelty discrimination and synaptophysin (SYN) expressions in both CA1 and CA3 of the hypoxia group prominently (p < 0.05). Nestin-expressing cells were reduced while vascular endothelial growth factor (VEGF) expression was enhanced in the subgranular zone (SGZ) of PS-hypoxia group (p < 0.05). VEGF enhancement triggered angiogenesis in the CA1 and CA3 significantly (p < 0.05). PS also increased thiobarbituric acid reactive substances (TBARS) levels in the hypoxia group as a result of oxidative stress (p < 0.05). Conclusion These findings demonstrated that PS exacerbates neurodevelopmental deficits in the hippocampus of acute hypoxia-induced offspring in adolescence.Öğe Antioxidant activity in melasma(Turkish Soc Dermatology Venerology, 2018) Ertam, Ilgen; Ozkapu, Tugce; Akcay, Yasemin; Sozmen, Eser Yildirim; Unal, IdilBackground and Design: Melasma is a common, symmetric hypermelanosis characterized by irregular brown to gray-brown macules on the face. It is frequently associated with pregnancy and oral contraceptive consumption. Sunlight and genetic factors play major roles in the pathogenesis of melasma. Human skin exposed to ultraviolet light or environmental oxidizing pollutants become a preferred target of oxidative stress. Topical and oral antioxidants are used to treat melasma. To investigate serum antioxidant capacity in patients with melasma and relationship between antioxidant levels and melasma severity. Materials and Methods: Forty-nine cases of melasma and 35 controls were included in the study. Each patient's skin pigmentation was assessed using the Melasma Area Severity Index (MASI) and mexameter reading. Serum trolox equivalent antioxidant capacity (TEAC), total antioxidant activity (TAOA), and ferric reducing power (FRAP) were evaluated in patients and controls by spectrophotometric method. Results: TEAC levels were higher in patients than in controls (p<0.00). However, there was no statistically significant relationship of MASI with TEAC, TAOA and, FRAP. Conclusion: According to our results, there is not a strong relationship between serum antioxidants and melasma severity. Therefore, we propose that antioxidant therapy may not be necessary in patients with melasma.Öğe Antioxidant and anti-inflammatory effects of biotechnologically transformed propolis(Wiley, 2018) Aldemir, Ozan; Yildirim, Hatice Kalkan; Sozmen, Eser YildirimThis study investigated the effect of biotransformation on anti-inflammatory activities of propolis. Propolis was dissolved with 10% ethanol and 40% polyethyleneglycol and applied ultrasonic treatment followed by Lactobacillus plantarum fermentation at 30 degrees C/48 hrs under constant agitation conditions. The in vitro anti-inflammatory activity was determined by measuring hyaluronidase and xanthine oxidase activity. Usage of PEG as a solvent and bioconversion by 8014 strain yielded high phenolic content, antioxidant, and anti-inflammatory activity. Results indicated that the anti-inflammatory activities of propolis samples are highly related to extraction methods. Optimization of extraction and biotransformation methods are subjects of further research. Practical applicationsIn our work, we have developed a new bio-product with reduced allergenicity by biotransformation from different Lactobacillus plantarum strains (10, 8014, ATCC) and propolis extracts prepared by dissolving in different solvents (ethanol, PEG, water). Anti-inflammatory and in-vitro antioxidant effects of this bio-product were investigated. As a result, it was found that in order to obtain a bio-product with high phenolic content and low allergic effect the most suitable bacterial strain was L. plantarum 8014 and the most suitable solvent was PEG. The most important factor limiting the use of propolis in humans is due to the allergenic components in the structure. It is thought that this new bio-product, which has low allergic effect and high antioxidant activity obtained by biotransformation of propolis, may be a new light of hope for people who can not use propolis due to allergic reasons. In the future, this bio-product is thought to provide significant benefits to human health.Öğe Antiproliferative effect of rosehip tea phenolics in prostate cancer cell lines(Walter De Gruyter Gmbh, 2020) Ozgonul, Ali Mert; Asik, Aycan; Durmaz, Burak; Aslaminabad, Ramin; Gunduz, Cumhur; Sozmen, Eser YildirimObjectives: Recently, phenolic compounds (quercetin, kaempferol, ellagic acid (EA), and myricetin) as natural sources have been suggested to be used for treatment and chemoprevention of prostate cancer. Since rosehip includes the above molecules in high concentration, we set out to investigate possible anti-proliferative effect of rosehip tea on the prostate cancer cell line. Methods: the flavonol content of rosehip tea prepared at different temperatures and time intervals was determined first and then the antiproliferative effect of tea samples was established by adding tea samples to the prostate cancer cell line (VCaP and LNCaP). Results: Quercetin was more effective in LNCaP cell than in VCaP cell (IC50 = 20 and 200 mu M, respectively). the boiled fruit shredded at minute 7 showed the highest levels of quercetin, EA and kaempferol and the boiled fruit at minute 7 had the highest levels of kaempferol and EA. the tea samples were prepared in concentrations relevant to their IC50 values, added to the VCaP and LNCaP cell lines. the antiproliferative effect of rosehip tea on VCaP cells was slightly greater than that of LNCaP cells. Conclusion: Each of the flavonols exhibits an antiproliferative effect. Our data clearly indicated that rosehip as a natural source of all flavonols had an antiproliferative effect on androgen-sensitive prostate cancer. Now that it is important to use natural sources in cancer, rosehip seems to be a promising natural product to be used to treat the prostate illness.Öğe Can cathepsin-D and galectin-3 be new inflammation biomarkers in detection of lysosomal diseases?(Academic Press Inc Elsevier Science, 2015) Ergun, Pelin; Kagnici, Mehtap; Ucar, Sema Kalkan; Coker, Mahmut; Akcay, Yasemin Delen; Sozmen, Eser YildirimÖğe Chitotriosidase as a possible marker of clinically evidenced atherosclerosis in dyslipidemic children(Walter De Gruyter Gmbh, 2014) Kologlu, Turan; Ucar, Sema Kalkan; Levent, Erturk; Akcay, Yasemin Delen; Coker, Mahmut; Sozmen, Eser YildirimA correlation has been clearly shown between inflammation markers and subclinical atherosclerosis markers in the early stages of atherogenesis in subjects with familial hypercholesterolemia (FH). The aim of this study was to investigate potential inflammation markers in the diagnosis of atherosclerosis in children with FH. A total of 48 dyslipidemic children and 24 healthy age-matched control subjects were taken into study. Inflammation and macrophage activation markers (hsCRP, myeloperoxidase, chitotriosidase, YKL-40, TNF-alpha, IL-6, IL-18, MMP-1 and MMP-9) and lipid parameters of all patients were measured. Carotid intima-media thickness (cIMT) and flow-mediated dilation (FMD) levels were determined. Our data suggested that clinically evidenced (by cIMT and FMD levels) atherosclerosis starts in the early ages in hypercholesterolemic children. Higher cholesterol levels strongly correlated with macrophage activation markers (ChT, YKL-40 and myeloperoxidase). ChT and YKL-40 seem to be the more predictable markers of atherosclerosis even in early ages (<6 years old) than other classical inflammation markers such as hs-CRP, IL-6 and TNF-alpha.Öğe Coexistence of Gaucher Disease and severe congenital neutropenia(Academic Press Inc Elsevier Science, 2019) Kose, Melis Demir; Canda, Ebru; Kagnici, Mehtap; Ucar, Sema Kalkan; Onay, Huseyin; Sozmen, Eser Yildirim; Karapinar, Deniz; Özkınay, Ferda; Coker, MahmutGaucher Disease (GD) is the most common lysosomal storage disorder has traditionally been classified into three clinical phenotypes. Type 3 GD is characterized by neurological involvement but neurological symptoms generally appear later in life than in type 2 disease. Neutropenia is much rarer than other hematological manifestations in GD and has not been scrutinized adequately. Severe congenital neutropenia (SCN) is a rare disease entity which is characterized by a paucity of peripherally circulating neutrophils with arrest of neutrophil maturation at the promyelocyte stage and consequent increased susceptibility to severe and recurrent infections. We report a patient who presented in the first year of life with visceral involvement and severe neutropenia in whom the propositus had a unique coexistence of Gaucher Disease and severe congenital neutropenia associated with a mutation in HAX1. In contrast to his expired siblings he had experienced no severe infections. These clinical observations suggest that enzyme replacement therapy may display a modulating factor with respect to the clinical course of SCN. Synopsis: Our patient is the only report of the combination of Gaucher Disease and Kostmann Syndrome in the literature. The clinical course of our patient is not severe when comparing with exitus siblings and other Kostmann Syndrome patients. But when considering the patient's only clinical difference is ERT, this case is very important to emphasise the role of enzyme replacement therapy in bone marrow.Öğe A critical review on human serum Paraoxonase-1 in the literature: truths and misconceptions(Walter De Gruyter Gmbh, 2021) Mackness, Michael; Sozmen, Eser YildirimHuman serum paraoxonase 1 (PON1) appears to play an important role in the development of a large variety of diseases with an inflammatory component including heart disease, diabetes, rheumatic diseases, neurological diseases and cancer. As such PON1 research is rapidly expanding into new biomedical fields. Unfortunately, this rapid expansion has resulted in a number of problems due to poor experimental design and the spreading of misconceptions in the literature. This review seeks to describe the basic properties of PON1 and the problems and misconceptions that have arisen.Öğe The decision-making levels of urine tetrasaccharide for the diagnosis of Pompe disease in the Turkish population(Walter De Gruyter Gmbh, 2020) Canbay, Erhan; Vural, Melisa; Ucar, Sema Kalkan; Sezer, Ebru Demirel; Karasoy, Hatice; Yuceyar, Ayse Nur; Sozmen, Eser YildirimBackground: Recently, urinary excretion of the tetrasaccharide 6-alpha-D-glucopyranosyl-maltotriose (Glc4) has been proposed as a marker for the diagnosis and monitoring of Pompe disease (PD). We aimed to determine the reference intervals and reliable decision-making levels of urine tetrasaccharide concentrations for the diagnosis of infantileand late-onset Pompe patients in the Turkish population. Methods: in this study, nine patients with PD (five of them with late-onset PD [LOPD]) and 226 healthy individuals (aged 0-64 years) were included. Urine Glc4 concentrations were determined using the ultra-high-performance liquid chromatography (UHPLC) tandem mass spectrometry (MS/MS) method. Results: Our data showed that the urine tetrasaccharide levels decreased with age in healthy individuals (p < 0.001, r = -0.256). It was higher especially during the first year of life compared to that in the elder subjects. the tetrasaccharide level of Pompe patients was higher compared to that of healthy controls of the same age: 99 +/- 68 mmol/mol creatinine for infantile onset vs. 4.0 +/- 3.0 mmol/mol creatinine for healthy controls of the same age group and 12.1 +/- 17.4 mmol/mol creatinine for late onset vs. 1.7 +/- 1.2 mmol/mol creatinine for healthy controls of the same age group. Conclusions: the results of this study showed that the reference intervals of tetrasaccharide in urine changed over time; therefore, it is critically important to define agebased decision levels for the diagnosis of LOPD.Öğe Development of a New Amperometric Biosensor for Measurement of Plasma Galactose Levels(Amer Chemical Soc, 2024) Canbay, Erhan; Sezer, Ebru; Canda, Ebru; Yazici, Havva; Ucar, Sema Kalkan; Coker, Mahmut; Sozmen, Eser YildirimGalactosemia is an inherited disease that occurs as a result of insufficient or no synthesis of some enzymes (GALT, GALK, and GALE) in galactose metabolism. Failure to make an early diagnosis, especially in newborns, can lead to severe clinical and even fatal consequences. The aim of this study is to develop a biosensor for measuring free galactose in plasma. The immobilization components of the developed free galactose biosensor are screen printed carbon electrode (SCPE), Prussian blue (PB), chitosan (CHIT), Nafion (NAF), gold nanoparticle (GNP), and galactose oxidase (GaOX). The CHIT/GaOX/NAF-GNP/GaOX/CHIT-GNP/SCPE-PB electrode showed a sensitive amperometric response to detect galactose. While the surface characterization of the biosensor was performed with cyclic voltammetry and scanning electron microscopy, the optimization and performance characterizations were made by applying an amperometry technique. The amperometric operating potential for the free galactose biosensor was determined as -0.05 V. The linear detection range for the free galactose biosensor is between 0.025 and 10 mM. This range includes galactose levels in plasma of both healthy and patients. The percent coefficient of variation values calculated for intraday and interday repeatability of the developed biosensor are below 10%. The practical use of the biosensor, for which optimization and characterization studies were carried out, was tested in 10 healthy 11 patients with galactosemia, and the results were compared with the colorimetric method. In conclusion, the unique analytical properties and effortless preparation of the new galactose biosensor developed in this study make them serious candidates for point-of-care diagnostic testing.Öğe The effect of temperature and light intensity during cultivation of Chlorella miniata on antioxidant, anti-inflammatory potentials and phenolic compound accumulation(Elsevier Science Bv, 2018) Sozmen, Alper Baran; Canbay, Erhan; Sozmen, Eser Yildirim; Ovez, BikemThe purpose of this study was to see if the microalgae species Chlorella miniata possess an antioxidant and antiinflammatorypotential and to see if there is a correlation between the cultivation conditions and these potentials. For this purpose, cultivation of the algae under different growth conditions had been carried out; afterwards the soxhletextraction of the produced biomass had been completed. Lastly the assays of Trolox equivalent antioxidant capacity (TEAC), ferric reducing antioxidant capacity (FARP), total antioxidant capacity (TAO), xanthine oxidase inhibition, and hyaluronidase inhibition were studied using extracts of microalgae obtained from the different cultivation parameters. Ifs important to mention that this was the first study using these assays on species of Chlorella miniata and the first that utilized the cultivation parameters on microalgae. The results were, 22,91 mg/g algae TEAC, 776,70 mg/g algae gallic acid equivalent of FRAP and 37,07% xanthine oxidase inhibition with a concentration of 10,5 mg algae per ml ethanol, 65,39% of hyaluronidase inhibition with a concentration of 0,9 mg algae per ml ethanol. The effects of the cultivation parameters on these assays and the Lc MS/MS analyses for various phenolic compounds in the extracts were also a part of the study.Öğe The effectiveness of interactive training and microlearning approaches on motivation and independent learning of medical students during the COVID-19 pandemic(Routledge Journals, Taylor & Francis Ltd, 2021) Sozmen, Eser Yildirim; Karaca, Ozan; Bati, A. HilalIn the COVID-19 pandemic period, most students felt lonely, accordingly their motivation for self-learning was low. The study aimed to increase their enthusiasm for work, and to improve their learning of biochemical subjects in the medical education programme during the pandemic period. Small parts of educational materials (microlearning) were presented in different formats such as videos, interactive education exercises, game-based quizzes and final exam was taken. The achievement of learning objectives of the course was evaluated by a survey. Students participating in daily activities gained higher self-confidence in learning and their final exam grade was higher compared to non-participating students (7.35 +/- 1.37 vs 7.06 +/- 1.88, p = 0.038). Most of the students agreed that daily activities (52%) increased their motivation to work. This approach might be proposed as a practice that supports distance learning, or even traditional education, especially in medical education that includes complex issues.Öğe The Effects of Vitamin D on Gentamicin-Induced Acute Kidney Injury in Experimental Rat Model(Hindawi Ltd, 2013) Hur, Ender; Garip, Alev; Camyar, Asuman; Ilgun, Sibel; Ozisik, Melih; Tuna, Sena; Olukman, Murat; Ozdemir, Zehra Narli; Sozmen, Eser Yildirim; Sen, Sait; Akcicek, Fehmi; Duman, SonerIntroduction. Acute kidney injury (AKI) pathogenesis is complex. Findings of gentamicin nephrotoxicity are seen in 30% of the AKI patients. Vitamin D has proven to be effective on renin expression, inflammatory response, oxidative stress, apoptosis, and atherosclerosis. We aimed to investigate the effect of vitamin D in an experimental rat model of gentamicin-induced AKI. Methods. Thirty nonuremic Wistar albino rats were divided into 3 groups: Control group, 1 mL saline intramuscular (im) daily; Genta group, gentamicin 100 mg/kg/day (im); Genta + vitamin D, gentamicin 100 mg/kg/day (im) in addition to 1 alpha, 25 (OH)(2)D-3 0.4 mcg/kg/day subcutaneously for 8 days. Blood pressures and 24-hour urine were measured. Blood urea and creatinine levels and urine tubular injury markers were measured. Renal histology was semiquantitatively assessed. Results. Urea, creatinine and urine neutrophil gelatinase-associated lipocalin, and kidney injury molecule-1 were all increased in Genta group indicating AKI model. Systolic blood pressure decreased, but urine volume and glutathione increased in Genta + Vit D group compared to Control group. Histological scores indicating tubular injury increased in Genta and Genta + Vit D groups. Conclusions. Vitamin D does not seem to be effective on histological findings although it has some beneficial effects via RAS system and a promising effect on antioxidant system.Öğe The Effects of Vitamin D on Gentamicin-Induced Acute Kidney Injury in Experimental Rat Model(Hindawi Ltd, 2013) Hur, Ender; Garip, Alev; Camyar, Asuman; Ilgun, Sibel; Ozisik, Melih; Tuna, Sena; Olukman, Murat; Ozdemir, Zehra Narli; Sozmen, Eser Yildirim; Sen, Sait; Akcicek, Fehmi; Duman, SonerIntroduction. Acute kidney injury (AKI) pathogenesis is complex. Findings of gentamicin nephrotoxicity are seen in 30% of the AKI patients. Vitamin D has proven to be effective on renin expression, inflammatory response, oxidative stress, apoptosis, and atherosclerosis. We aimed to investigate the effect of vitamin D in an experimental rat model of gentamicin-induced AKI. Methods. Thirty nonuremic Wistar albino rats were divided into 3 groups: Control group, 1 mL saline intramuscular (im) daily; Genta group, gentamicin 100 mg/kg/day (im); Genta + vitamin D, gentamicin 100 mg/kg/day (im) in addition to 1 alpha, 25 (OH)(2)D-3 0.4 mcg/kg/day subcutaneously for 8 days. Blood pressures and 24-hour urine were measured. Blood urea and creatinine levels and urine tubular injury markers were measured. Renal histology was semiquantitatively assessed. Results. Urea, creatinine and urine neutrophil gelatinase-associated lipocalin, and kidney injury molecule-1 were all increased in Genta group indicating AKI model. Systolic blood pressure decreased, but urine volume and glutathione increased in Genta + Vit D group compared to Control group. Histological scores indicating tubular injury increased in Genta and Genta + Vit D groups. Conclusions. Vitamin D does not seem to be effective on histological findings although it has some beneficial effects via RAS system and a promising effect on antioxidant system.Öğe Evaluation of endothelial function and platelet activation in dyslipidemic children(Dr Behcet Uz Cocuk Hastaliklari Ve Cerrahisi, 2016) Kalkan Ucar, Sema; Delen Akcay, Yasemin; Kologlu, Turan; Levent, Erturk; Sozmen, Eser Yildirim; Coker, MahmutObjective: The aim of this study was to determine the parameters of endothelial function and platelet activation in children with familial hypercholesterolemia by measuring plasma homocysteine, asymmetrical dimethyl arginine (ADMA), nitrotyrosine and P-selectin levels. Methods: Thirty-five heterozygous familial hypercholesterolemic patients on statin therapy, 10 homozygous familial hypercholesterolemic patients treated by LDL apheresis and lipid-lowering drugs, and 25 healthy children, all aged between 2 to 16 years were enrolled in this study. Echocardiography was performed and intima-media thickness (IMT), and endothelium-dependent vasodilation parameters were evaluated. LDL apheresis was performed by adsorption method using double-membrane filtration technique. Plasma nitrotyrosine, homocysteine, P-selectin and ADMA levels were determined with an enzyme-linked immunosorbent assay (ELISA) using a commercial kit. Results: Plasma homocysteine (p=0.000), ADMA (p=0.005), nitrotyrosine (p=0.808), p-selectin (p=0.466) levels were lowest in the LDL apheresis group. A positive correlation was detected between homocysteine and intima/media thickness (r=0.334, p=0.043). Showed that LDL apheresis therapy might decrease plasma levels of homocysteine, ADMA, and nitrotyrosine, and might eventually play an important role in the improvement of endothelial dysfunction and platelet activity. Conclusion: Our data showed that at post-LDL apheresis status the homozygous hyperlipidemic children have lower levels of homocysteine, ADMA, and nitrotyrosine, compared with the heterozygous hyperlipidemic children.Öğe False Positive Diagnosis of Lysosomal Storage Disease Based on Dried Blood Spot Sample; Leucocyte Number of a Challenging Factor(Galenos Yayincilik, 2018) Sozmen, Eser Yildirim; Dondurmaci, Meral; Ucar, Sema Kalkan; Coker, MahmutAim: Recently dried blood spot (DBS) samples have been recommended as a screening test for Lysosomal Storage diseases. Although DBS samples have many advantages including non-invasiveness, cost and transportation, usage of these samples is limited by its high false positive rate. We aimed to investigate any possible effect of the leucocyte number on enzyme activity in dried blood samples in a retrospective study. Materials and Methods: Data was collected from subjects (n=263) for whom hematological parameters were available in the database of Ege University Hospital. The lysosomal enzyme activity results (alpha glycosidase, glycocerebrosidase, alpha galactosidase, sphingomyelinase and galactocerebrosidase) were re-evaluated with regard to the leucocyte number. Enzyme activities were measured using fluorometric and liquid chromatography-tandem mass spectrometry methods. Results: All enzyme activities closely correlated with the total number of leucocyte, since leucocytes are the main source of lysosomal enzymes. Glycocerebrosidase and galactocerebrosidase presented a positive correlation with the number of neutrophils and sphingomyelinase showed a positive correlation with the number of lymphocytes. When we recalculated the lysosomal enzyme activities with regard to the leucocyte number, the false positive rates for glycocerebrosidase, sphingomyelinase and alpha galactosidase decreased from 20%, 10.5% and 10.8% to 4.5%, 4.4% and 4.2%, respectively. Conclusions: Our data indicated that the enzyme activity in dried blood samples including low leucocyte number might be found lower than reference intervals resulting in false positive diagnosis. We concluded that the calculation of enzyme activity with regard to the number of leucocytes might produce more reliable results and might be helpful in decreasing the false positive rate.Öğe High chitotriosidase and AGE levels in acromegaly: a case-control study(Springer Int Publ Ag, 2022) Ozisik, Hatice; Yurekli, Banu Sarer; Suner, Asli; Copur, Oznur; Sozmen, Eser Yildirim; Ozbek, Suha Sureyya; Karabulut, Ahmet KasimPurpose Acromegaly is associated with oxidative stress and inflammation parameters. Chitotriosidase (CHITO) is a marker of macrophage activation and plays a pivotal role in the activation of inflammatory and immunological responses. Our study aimed to determine CHITO,YKL-40, advanced glycation end product (AGE), and high-sensitivity C-reactive protein (hsCRP) levels to investigate malondialdehyde (MDA), catalase, superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) activities and to evaluate any association of these parameters with carotid intima media thickness (cIMT) in patients with controlled acromegaly. Methods Thirty controlled acromegaly patients and 41 age- and sex-matched control cases were studied. We obtained demographic data, hormonal and metabolic parameters, and cIMT. CHITO activity was measured with the fluorometric method of Chamoles et al. YKL-40 and hsCRP levels were measured using ELISA. AGEs were measured based on spectrofluorimetric detection. GSH-Px activity was determined by a colorimetric assay. MDA, SOD, and catalase activities were determined in hemolysis. Results Higher CHITO, AGE, and hsCRP concentrations were observed in patients with acromegaly compared to controls. SOD levels were non-significantly higher in the acromegaly group, while catalase activities were lower in patients with acromegaly. Correlation analyses of CHITO, AGEs, YKL-40, hsCRP, MDA, catalase, GSH-Px, and SOD with metabolic, anthropometric, and laboratory parameters did not demonstrate any significant correlation (p > 0.05). There was no significant difference between groups with regard to cIMT levels. Conclusion This is the first study investigating CHITO and AGE levels in patients with acromegaly. Serum CHITO, AGE, and hsCRP levels in acromegalic patients were significantly increased. It may be important to evaluate CHITO, AGE, and hsCRP levels in acromegalic patients who are already under cardiometabolic surveillance due to risk of developing cardiovascular disease.Öğe How Preeclampsia Affects Oxidant Status and Antiiflammatory Potential of Breast Milk?(Elsevier Science Inc, 2016) Demir, Damla; Sezer, Ebru Demirel; Turan, Volkan; Ozturk, Sahin; Canbay, Erhan; Sozmen, Eser YildirimÖğe Inflammatory responses in esophageal mucosa before and after laparoscopic antireflux surgery(Baishideng Publishing Group Inc, 2024) Ergun, Pelin; Kipcak, Sezgi; Gunel, Nur Selvi; Sozmen, Eser Yildirim; Bor, SerhatBACKGROUND Currently, the primary treatment for gastroesophageal reflux is acid suppression with proton pump inhibitors, but they are not a cure, and some patients don't respond well or refuse long-term use. Therefore, alternative therapies are needed to understand the disease and develop better treatments. Laparoscopic anti-reflux surgery (LARS) can resolve symptoms of these patients and plays a significant role in evaluating esophageal healing after preventing harmful effects. Successful LARS improves typical gastroesophageal reflux symptoms in most patients, mainly by reducing the exposure time to gastric contents in the esophagus. Amelioration of the inflammatory response and a recovery response in the esophageal epithelium is expected following the cessation of the noxious attack. AIM To explore the role of inflammatory biomolecules in LARS and assess the time required for esophageal epithelial recovery. METHODS Of 22 patients with LARS (pre- and post/5.8 +/- 3.8 months after LARS) and 25 healthy controls (HCs) were included. All subjects underwent 24-h multichannel intraluminal impedance-pH monitoring and upper gastrointestinal endoscopy, during which esophageal biopsy samples were collected using endoscopic techniques. Inflammatory molecules in esophageal biopsies were investigated by reverse transcription-polymerase chain reaction and multiplex-enzyme-linked immunosorbent assay. RESULTS Post-LARS samples showed significant increases in proinflammatory cytokines [interleukin (IL)-1 beta, interferon-gamma, C-X-C chemokine ligand 2 (CXCL2)], anti-inflammatory cytokines [CC chemokine ligand (CCL) 11, CCL13, CCL17, CCL26, CCL1, CCL7, CCL8, CCL24, IL-4, IL-10], and homeostatic cytokines (CCL27, CCL20, CCL19, CCL23, CCL25, CXCL12, migration inhibitory factor) compared to both HCs and pre-LARS samples. CCL17 and CCL21 levels were higher in pre-LARS than in HCs (P < 0.05). The mRNA expression levels of AKT1, fibroblast growth factor 2, HRAS, and mitogen-activated protein kinase 4 were significantly decreased post-LARS vs pre-LARS. CCL2 and epidermal growth factor gene levels were significantly increased in the pre-LARS compared to the HCs (P < 0.05). CONCLUSION The presence of proinflammatory proteins post-LARS suggests ongoing inflammation in the epithelium. Elevated homeostatic cytokine levels indicate cell balance is maintained for about 6 months after LARS. The anti-inflammatory response post-LARS shows suppression of inflammatory damage and ongoing postoperative recovery.Öğe LC-MS/MS measurement of leukocyte cystine; effect of preanalytic factors(Elsevier, 2020) Canbay, Erhan; Sezer, Ebru Demirel; Ucar, Sema Kalkan; Coker, Mahmut; Sozmen, Eser YildirimCystinosis is an autosomal recessive disorder characterized by the accumulation of cystine in lysosomes, causing irreversible damage to organs, especially the kidneys. Intracellular leukocyte cystine concentrations are used to diagnose cystinosis and to monitor cysteamine treatment. the aim of this study was to develop and validate a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method without derivatization capable of measuring leukocyte intracellular cystine concentrations. During development, the effects of using three different protein precipitation agents were evaluated in terms of sensitivity and the matrix effect, with 12% trichloroacetic acid providing the highest sensitivity. the effects of different blood collection tubes were also assessed in terms of recovery, matrix effect, and protein content. Compared to other methods, our method was quicker (run time of 3 min), was linear over the range 0.078-100 mu M, and had lower limits of detection (0.0192 mu M) and quantification (0.0582 mu M). the intra-day and inter-day reproducibility %CVs were <= 10%. and the method had excellent recovery rates (94%-106%). Other parameters including matrix selectivity, injection carryover, leukocyte lysate stability were also validated and met the acceptance criterias of European Medicines Agency (EMA) Guideline. the assay was successfully applied to quantify cystine leukocyte concentration in healthy and cystinosis patients.