Yazar "Somer, Nehir Ünver" seçeneğine göre listele

Listeleniyor 1 - 3 / 3
  • Küçük Resim Yok
    Öğe
    GC/MS Analysis of Alkaloids in Galanthus fosteri Baker and Determination of Its Anticholinesterase Activity
    (2020) Emir, Ahmet; Emir, Ceren; Bozkurt, Buket; Somer, Nehir Ünver
    Objectives: Amaryllidaceae alkaloids are well known for their wide range of pharmacological activities. Galanthamine, an Amaryllidaceae alkaloid, is an effective, selective, reversible, and competitive cholinesterase inhibitor marketed under different commercial names in several countries for the treatment of Alzheimer’s disease. The aim of this work was to study the alkaloid profiles of the aerial parts and bulbs of both flowering and fruiting periods of Galanthus fosteri Baker (Amaryllidaceae), as well as analyzing their inhibitory activities on both acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) for the first time. Materials and Methods: The alkaloid profiles of the four samples were determined by means of gas chromatography-mass spectrometry, and AChE and BuChE inhibition assays were performed by the modified Ellman method. Results: Totally, 22 compounds with mass spectral characteristics of Amaryllidaceae alkaloids were detected in the extracts. Significant AChE and BuChE inhibitory activities were observed in the tested samples (IC50 between 0.189 and 91.23 µg/mL). Conclusion: This study shows that G. fosteri, collected from Akdağ, Amasya (Turkey), is a potential source of diverse chemical structures of Amaryllidaceae alkaloids with cholinesterase inhibitory properties.
  • Küçük Resim Yok
    Öğe
    GC/MS Analysis of Alkaloids in Galanthus fosteri Baker and Determination of Its Anticholinesterase Activity
    (2020) Emir, Ahmet; Emir, Ceren; Bozkurt, Buket; Somer, Nehir Ünver
    Objectives: Amaryllidaceae alkaloids are well known for their wide range of pharmacological activities. Galanthamine, an Amaryllidaceae alkaloid, is an effective, selective, reversible, and competitive cholinesterase inhibitor marketed under different commercial names in several countries for the treatment of Alzheimer’s disease. the aim of this work was to study the alkaloid profiles of the aerial parts and bulbs of both flowering and fruiting periods of Galanthus fosteri Baker (Amaryllidaceae), as well as analyzing their inhibitory activities on both acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) for the first time. Materials and Methods: the alkaloid profiles of the four samples were determined by means of gas chromatography-mass spectrometry, and AChE and BuChE inhibition assays were performed by the modified Ellman method. Results: Totally, 22 compounds with mass spectral characteristics of Amaryllidaceae alkaloids were detected in the extracts. Significant AChE and BuChE inhibitory activities were observed in the tested samples (IC50 between 0.189 and 91.23 µg/mL). Conclusion: This study shows that G. fosteri, collected from Akdağ, Amasya (Turkey), is a potential source of diverse chemical structures of Amaryllidaceae alkaloids with cholinesterase inhibitory properties.
  • Küçük Resim Yok
    Öğe
    Quantitative analysis of lycorine in Galanthus alpinus Sosn. var. alpinus by HPLC-DAD
    (2018) Emir, Ceren; Somer, Nehir Ünver
    in this study, a reversed-phase, high pressure liquid chromatography (RP-HPLC) method was applied in the quantitative analysis of lycorine in the aerial parts and bulbs of Galanthus alpinus Sosn. var. alpinus collected from Rize during the flowering and fruiting seasons. A simple method to extract lycorine from the plant specimens was performed, using pre-packed columns with diatomaceous earth (Extrelut®). the chromatographic separation was achieved employing an isocratic system with a mobile phase including trifluoroacetic acid-water-acetonitrile (0.01:95:5) measured at a flow rate of 1 mL/min using a diode array detector (DAD). the lycorine content in the bulbs during the flowering and fruiting seasons was detected as 0.01576 and 0.02351 %, respectively. Also, validation studies showed that the method was specific, accurate and precise.