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Öğe DETERMINATION OF FETAL RHESUS D STATUS BY MATERNAL PLASMA DNA ANALYSIS(Macedonian Acad Sciences Arts, 2013) Aykut, A.; Onay, H.; Sagol, S.; Gunduz, C.; Özkınay, Ferda; Cogulu, O.In this study, we assessed the feasibility of fetal RhD genotyping by analysis of cell-free fetal DNA(cffDNA) extracted from plasma samples of Rhesus (Rh) D-negative pregnant women by using real-time polymerase chain reaction (PCR). Fetal genotyping was performed on 30 RhD-negative women between 9 and 39 weeks of gestation who were referred to us for invasive testing [amniocentesis/chorionic villi sampling (CVS)]. The fetal RHD genotype was determined based on real-time PCR method. Exons 7 and 10 of the RHD and SRY genes were targeted. Among the pregnant women, 12 were carrying male and 17 were carrying female fetuses. Out of 29 pregnant women, 21 had RhD-positive and nine had RhD-negative fetuses. One sample) case 12, whose blood group was found to be AB Rh [+] (was excluded due to controversial results from repeated serological analyses. All prenatal results were in concordance with postnatal RhD status and fetal sex without false-positive or -negative results. Performing real-time PCR on cffDNA showed accurate, efficient and reliable results, allowing rapid and high throughput non invasive determination of fetal sex and RhD status in clinical samples.Öğe DETERMINATION OF FETAL RHESUS D STATUS BY MATERNAL PLASMA DNA ANALYSIS(Macedonian Acad Sciences Arts, 2013) Aykut, A.; Onay, H.; Sagol, S.; Gunduz, C.; Özkınay, Ferda; Cogulu, O.In this study, we assessed the feasibility of fetal RhD genotyping by analysis of cell-free fetal DNA(cffDNA) extracted from plasma samples of Rhesus (Rh) D-negative pregnant women by using real-time polymerase chain reaction (PCR). Fetal genotyping was performed on 30 RhD-negative women between 9 and 39 weeks of gestation who were referred to us for invasive testing [amniocentesis/chorionic villi sampling (CVS)]. The fetal RHD genotype was determined based on real-time PCR method. Exons 7 and 10 of the RHD and SRY genes were targeted. Among the pregnant women, 12 were carrying male and 17 were carrying female fetuses. Out of 29 pregnant women, 21 had RhD-positive and nine had RhD-negative fetuses. One sample) case 12, whose blood group was found to be AB Rh [+] (was excluded due to controversial results from repeated serological analyses. All prenatal results were in concordance with postnatal RhD status and fetal sex without false-positive or -negative results. Performing real-time PCR on cffDNA showed accurate, efficient and reliable results, allowing rapid and high throughput non invasive determination of fetal sex and RhD status in clinical samples.Öğe Determining emergency caesarean risk factors in placenta previa cases: a prospective cohort study(EDRA S.p.A, 2023) Arı, S.A.; Okmen, F.; Akdemir, A.; Yeniel, A.O.; Ergenoğlu, A.M.; Sagol, S.Objective. The aim of the current study was to determine the emergency caesarean section risk factors for patient with placenta previa. Materials and Methods. A total of 98 placenta previa cases were enrolled the current prospective cohort study between November 2018-June 2019. The time, number and frequency of vaginal bleeding episodes, emergency caesarean section requirement, presence of adhesive pathologies was recorded. Results. The mean cervical length was 35.7 (± 8.3) mm. Patients with presence of vaginal bleeding before the 28th week (OR 11 (95%CI 1.85-65.07); p < 0.001); participants who had two or more bleeding occurrences (OR 7.39 (95%CI 1.83-29.80); p = 0.001); patients with cervical length measurements < 30 mm (OR 2.91 (95% CI 0.64-13.14); p = 0.039) had higher risk of emergency caesarean section. Emergency caesarean section rate was 12%. No relation was shown between the emergency caesarean section and invasion pathologies in cases with placenta previa (p = 0.241). Conclusions. The risk of emergency caesarean section is high in patients who had a vaginal bleeding before the 28th gestational week, had two or more bleeding episodes throughout their pregnancy and patients with cervical length was less than 30 mm. © The Authors.Öğe Evaluation of VEGF in placental bed biopsies from preeclamptic women by immunohistochemistry(I R O G Canada, Inc, 2007) Cirpan, T.; Akercan, F.; Terek, M. C.; Kazandi, M.; Ozcakir, H. T.; Giray, G.; Sagol, S.Objective: The aim of the study was to determine VEGF protein with immunohistochemical staining in placental bed biopsies of preeclamptic pregnancies in comparison to normal controls. Design: Prospective cohort Study. Methods: The placental bed biopsies were obtained from 12 patients with preeclapmsia and ten patients for a control group at the time of cesarean delivery. Tissue samples of the placental bed were examined for VEGF protein distribution with avidin-biotin-peroxidase immunohistochemistry. Two blinded histopathologists were asked to score each sample for the intensity of staining and the number of cells stained in a randomly selected HPF of each sample. The resulting "H-score" was computed as a product of intensity and percent of cells stained. Results: VEGF expression was significantly lower in both the myometrium and stroma of the preeclamptic group compared to the control group (77.2 +/- 25.4 vs 134 +/- 44.3, p = 0.007; 194.1 +/- 20.7 vs 170.2 +/- 17, p = 0.017, respectively). Conclusion: VEGF expression is significantly lower in placental bed biopsies of preeclamptic pregnancies.Öğe MMAC tumor supressor gene expression in ovarian endometriosis and ovarian adenocarcinoma(I R O G Canada, Inc, 2007) Cirpan, T.; Aygul, S.; Terek, M. C.; Kazandi, M.; Dikmen, Y.; Zekioglu, O.; Sagol, S.Objective: The aim of this study was to investigate the role of MMAC1 protein in the relationship between ovarian endometriosis and clear cell and endometrioid-type ovarian adenocarcinomas. Methods: A total of 63 subjects who underwent surgery for a pelvic tumoral mass, 30 of whom were diagnosed with grade 1 to 3 ovarian adenocarcinoma and 33 of whom were diagnosed with grade 1 to 4 endometriosis during histopathological examination were included in this study. The mean age for subjects with ovarian endometrioid type adenocarcinoma was 51.8 +/- 12.4, whereas the mean age for subjects with ovarian clear cell type adenocarcinoma was 59.5 +/- 13.7. Ovarian carcinomas were graded in accordance with the FIGO 1989 grading system. The mean age for subjects with endometriosis was 37 +/- 11.9. New sections were obtained from paraffin blocks in the archives of Ege University, School of Medicine, Department of Pathology onto lysinated slides and immunohistochemical staining by using mouse monoclonal antibody (MMAC1, 28H6 clone, Novocastra, UK) as MMAC antibody was applied in order to determine MMAC1 protein. Brown staining on the nucleus was considered as positive immunoreactivity. Immunoreactive staining was evaluated as percentage staining over the whole preparative. Results: Of the 63 subjects included in the immunohistochemical study, ovarian endometrioid adenocarcinoma was identified in 18 subjects, while 12 subjects were diagnosed with ovarian clear cell adenocarcinoma and 33 subjects with ovarian endometriosis. No significant relationships were observed between age and MMAC immune staining in the ovarian endometrioid adenocarcinoma (r = -0.41, p = 0.08) and ovarian endometriosis (r = 0.12, p = 0.50) groups, whereas a significant relationship was observed in the ovarian clear cell adenocarcinoma group (r = 0.631, p = 0.02). No significant relationships were observed between CA125 levels and MMAC immune staining in the ovarian endometrioide adenocarcinoma (r = 0.056, p = 0.82), ovarian endometriosis (r = 0.21, p = 0.36) and ovarian clear cell adenocarcinoma (r=0.363, p=0.24) groups. No correlations were observed between endometriosis stages and the MMAC immune staining (r = -0. 17, p = 0.92). There was no correlation between mean diameter of endometrioma and MMAC immune staining (r = -0.230, p = 198). Mean endometrioma diameter was 5.7 +/- 3.5 (1-15.5). No correlations were detected between MMAC immune staining and ovarian endometrioide adenocarcinoma or ovarian clear cell adenocarcinoma stage (r = -0.22, p = 0.37; r = 0.44, p = 0.14, respectively). No significant relationships with respect to MMAC immune staining were detected between the endometriosis and ovarian clear cell adenocarcinoma groups (p = 0.05) and between the ovarian clear cell adenocarcinoma and ovarian endometrioid adenocarcinoma groups (p = 0.27). A significant relationship with respect to MMAC immune staining was observed between ovarian endometrioide adenocarcinoma and endometriosis groups (p = 0.001). Conclusion: Immunohistochemical determination of MMAC defective protein expressions could be considered for utilization as a new, simple and useful technique in determination of endometriosis patients with increased risk of malignant transformation, patients where early surgical treatment would be necessary and patients that should be subjected to follow-up controls with a higher frequency.Öğe The role of functional polymorphisms of matrix metalloproteinases 2 and 9 in spontaneous abortion samples(Wiley-Blackwell, 2016) Ataman, E.; Pariltay, E.; Hazan, F.; Kirbiyik, O.; Sagol, S.; Özkınay, Ferda; Cogulu, O.
