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Öğe Beneficial effects of agomelatine in experimental model of sepsis-related acute kidney injury(Turkish Assoc Trauma Emergency Surgery, 2016) Basol, Nursah; Erbas, Oytun; Cavusoglu, Turker; Meral, Ayfer; Ates, UtkuBACKGROUND: Sepsis-related acute kidney injury (AKI) is a serious complication of sepsis. Problems persist regarding early diagnosis and treatment of AKI. The aim of the present study was to evaluate the efficacy of agomelatine, which is primarily known for its positive effects on depressive and anxiety disorders in sepsis-related AKI. METHODS: Sepsis model was created with cecal ligation puncture (CLP). Rats were separated into 4 groups of 8 each: the control group, the sham-operated group, the CLP+saline group, and the CLP+agomelatine group. Agomelatine was administered intraperitoneally in doses of 20 mg/kg. RESULTS: In the agomelatine group, reductions were observed in levels of tumor necrosis factor alpha (TNF-alpha), malondialdehyde (MDA), blood urea nitrogen (BUN), and creatinine, as well as in histological kidney scores, compared to the non-treated group. In addition, it was demonstrated that agomelatine treatment had positive effect on sepsis-induced morphological damage to renal and tubular tissues. CONCLUSION: Agomelatine showed strong efficacy in sepsis-related AKI, demonstrated with histological and biochemical results in an experimental model. It is believed that antioxidant and pro-inflammatory effects of agomelatine are responsible for the improvement in kidneys.Öğe Diyabetik Kardiyomiyopati Sıçan Modelinde Oksitosin Etkilerinin Histolojik ve Biyokimyasal Olarak İncelenmesi(2017) Çavuşoğlu, Türker; Çiftçi, Öznur Dilek; Çağıltay, Eylem; Meral, Ayfer; Kızıloğlu, İlker; Gürgül, Serkan; Uyanıkgil, Yiğit…Öğe Effects of Silk Sericin on Incision Wound Healing in a Dorsal Skin Flap Wound Healing Rat Model(Int Scientific Literature, Inc, 2016) Ersel, Murat; Uyanikgil, Yigit; Akarca, Funda Karbek; Ozcete, Enver; Altunci, Yusuf Ali; Karabey, Fatih; Cavusoglu, Turker; Meral, Ayfer; Yigitturk, Gurkan; Cetin, Emel OykuBackground: The wound healing process is complex and still poorly understood. Sericin is a silk protein synthesized by silk worms (Bombyx mori). The objective of this study was to evaluate in vivo wound healing effects of a sericin-containing gel formulation in an incision wound model in rats. Material/Methods: Twenty-eight Wistar-Albino rats were divided into 4 groups (n=7). No intervention or treatment was applied to the Intact control group. For other groups, a dorsal skin flap (9x3 cm) was drawn and pulled up with sharp dissection. The Sham operated group received no treatment. The Placebo group received placebo gel without sericin applied to the incision area once a day from day 0 to day 9. The Sericin Group 3 received 1% sericin gel applied to the incision area once a day from day 0 to day 9. Hematoxylin and eosin stain was applied for histological analysis and Mallory-Azan staining was applied for histoimmunochemical analysis of antibodies and iNOS (inducible nitric oxide synthase), and desmin was applied to paraffin sections of skin wound specimens. Parameters of oxidative stress were measured in the wound area. Results: Epidermal thickness and vascularization were increased, and hair root degeneration, edema, cellular infiltration, collagen discoloration, and necrosis were decreased in Sericin group in comparison to the Placebo group and the Sham operated group. Malonyldialdehyde (MDA) levels were decreased, but superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activities were increased in the sericin group. Conclusions: We found that sericin had significant positive effects on wound healing and antioxidant activity. Sericin-based formulations can improve healing of incision wounds.Öğe Exogenously administered adenosine attenuates renal damage in streptozotocin-induced diabetic rats(Taylor & Francis Ltd, 2016) Taskiran, Emin; Erbas, Oytun; Yigitturk, Gurkan; Meral, Ayfer; Akar, Harun; Taskiran, DilekBackground: Diabetic nephropathy (DNP) is one of the most serious complications of diabetes mellitus (DM). In the present study, we investigated the potential of adenosine as a therapeutic candidate for preventing DNP.Methods: Twenty-one adult male rats were included in the study. Fourteen rats were administered a single dose of 60mg/kg streptozotocin (STZ) to induce diabetes. Seven rats served as normal control group. Diabetic rats were randomly divided into two groups: one group was treated with 1mL/kg saline/day (DM+saline) and the other group was treated with 5mg/kg/day adenosine (DM+adenosine) for 6weeks. After 6weeks, biochemical parameters including urea, creatinine, blood urea nitrogen (BUN), kidney injury molecule-1 (KIM-1) and tumor necrosis factor- (TNF-) were measured in plasma samples. Also, kidneys were removed for histopathological assessment.Results: Both of plasma KIM-1 and TNF- levels were significantly higher in DM+saline group compared to controls. However, treatment of diabetic rats with adenosine significantly decreased the plasma KIM-1 and TNF- levels compared to DM+saline group. Significant histopathological changes were observed in diabetic rats whereas adenosine treatment effectively prevented these changes.Conclusions: The findings of the present study suggest that adenosine may be a useful therapeutic agent for preventing DNP.Öğe Neurobehavioral effects of long-term maternal fructose intake in rat offspring(Pergamon-Elsevier Science Ltd, 2018) Erbas, Oytun; Erdogan, Mumin Alper; Khalilnezhad, Asghar; Gurkan, Fulya Tuzcu; Yigitturk, Gurkan; Meral, Ayfer; Taskiran, DilekBackground: Previous studies have indicated an association between maternal metabolic conditions and general developmental disturbances of the offspring. Objective: We aimed to investigate the influence of long-term maternal fructose intake during gestation and lactation on neurobehavioral development of rat offspring. Methods: Twelve female Sprague Dawley rats were received either 30% fructose enriched water (n = 6) or regular tap water (control, n = 6) for 12 weeks. Then, control and fructose-received females were caged with a fertile male, and received 30% fructose and regular chow throughout pregnancy, delivery and until offspring's weaning. On P21, forty littermates (10 male control, 10 female control, 10 male fructose and 10 female fructose) were separated and housed with ad libitum access to standard food and tap water. Following behavioral evaluations at P50, brain levels of TNF-alpha, neuregulin 1 (NRG1), glutamic acid decarboxylase 67 (GAD67), nerve growth factor (NGF), insulin-like growth factor 1 (IGF-1), and 5-hydroxyindoleacetic acid (5-HIAA) were measured. Histologically, hippocampal neuronal density and GFAP expression were assessed. Results: Analysis of the behavioral tests (three-chamber social test, open field test, passive avoidance learning test and stereotypy test) revealed significant differences among the groups. Histologically, hippocampal CA1 and CA3 regions displayed significant alterations such as gliosis and neuronal cell death in fructose-exposed groups compare to controls. Biochemical measurements of the brain levels of TNF-alpha and neurodevelopmental markers showed significant differences between controls and fructose-exposed groups. Conclusion: These results suggest a possible link between the chronic maternal metabolic stress, such as long-term fructose intake, and neurodevelopmental disturbances in the offspring.Öğe Neuroprotective Effects of Eexenatide in a Rotenone-Induced Rat Model of Parkinson's Disease(Elsevier Science Inc, 2017) Aksoy, Durdane; Solmaz, Volkan; Cavusoglu, Turker; Meral, Ayfer; Ates, Utku; Erbas, OytunBackround: Several studies suggest an association between Parkinson's disease (PD) and type 2 diabetes mellitus; these 2 diseases are both known to affect the common molecular pathways. As a synthetic agonist for the glucagon-like peptide 1 receptor, exenatide has been evaluated as a neuroprotective agent in multiple animal models. Rotenone models of PD have great potential for the investigation of PD pathology and motor and nonmotor symptoms, as well as the role of gene environment interactions in PD causation and pathogenesis. Therefore, in this study, the neurochemical, behavioral and histologic effects of exenatide on a rotenone-induced rat model of PD were examined. Materials and Methods: Eighteen adult male rats were randomly divided into the following 3 groups (n = 6): 1 group received stereotaxical infusion of dimethyl sulfoxide (vehicle, group 1) and the others received stereotaxical infusion of rotenone (groups 2 and 3). Apomorphine-induced rotation test was applied to the rats after 10 days. Thereafter, group 2 was administered isotonic saline, whereas group 3 was administered exenatide for 28 days. Results: Malondialdehyde and tumor necrosis factor alpha levels increased in the rats with PD induced by rotenone, whereas malondialdehyde and tumor necrosis factor alpha levels markedly decreased in the rats treated with exenatide. The apomorphine-induced rotation test scores of exenatide-treated rats were determined to be lower compared with the untreated group. Additionally, treatment with exenatide significantly reduced the loss of dopaminergic neurons in striatum. Conclusions: These results have shown that exenatide has neuroprotective, anti-inflammatory and antioxidant effects in a rotenone-induced rat model of PD.Öğe Regression of experimental endometriotic implants in a rat model with the angiotensin II receptor blocker losartan(Wiley, 2015) Cakmak, Bulent; Cavusoglu, Turker; Ates, Utku; Meral, Ayfer; Nacar, Mehmet Can; Erbas, OytunAimEndometriosis is a common disease in women of reproductive age, and many different treatments have been developed, although none has provided a cure. In this study, the efficacy of losartan, an angiotensin II type 1 receptor blocker and an antiangiogenic and anti-inflammatory agent, on regression of experimental endometriotic implants in a rat model was investigated. MethodsPeritoneal endometriosis was surgically induced in 16 mature female Sprague-Dawley rats. The peritoneal endometriotic implant was confirmed after 28 days, and the animals were divided randomly into two groups. The control group (n=8) was given 4mL/day tap water by oral gavage, and the losartan group (n=8) was given 20mg/kg per day losartan p.o. We compared endometriotic implant size, extent and severity of adhesion, as well as plasma and peritoneal lavage fluid cytokine levels including vascular endothelial growth factor (VEGF) and tumor necrosis factor (TNF)-, plasma inflammatory factor pentraxin-3 (PTX-3) and C-reactive protein (CRP) between the treatment groups. ResultsMean surface endometriotic area, histological score of implants, adhesion formation, plasma VEGF, TNF, PTX-3 and CRP levels were significantly lower in the losartan group compared with control (P<0.05). Furthermore, the peritoneal VEGF level was lower in the losartan group than in the control group (P<0.001), but peritoneal TNF- was similar in both groups (P>0.05). ConclusionLosartan suppressed the implant surface area of experimental endometriosis in rats and reduced the levels of plasma VEGF, TNF-, PTX-3 and CRP.Öğe Regression of experimental endometriotic implants in a rat model with the angiotensin II receptor blocker losartan(Wiley, 2015) Cakmak, Bulent; Cavusoglu, Turker; Ates, Utku; Meral, Ayfer; Nacar, Mehmet Can; Erbas, OytunAimEndometriosis is a common disease in women of reproductive age, and many different treatments have been developed, although none has provided a cure. In this study, the efficacy of losartan, an angiotensin II type 1 receptor blocker and an antiangiogenic and anti-inflammatory agent, on regression of experimental endometriotic implants in a rat model was investigated. MethodsPeritoneal endometriosis was surgically induced in 16 mature female Sprague-Dawley rats. The peritoneal endometriotic implant was confirmed after 28 days, and the animals were divided randomly into two groups. The control group (n=8) was given 4mL/day tap water by oral gavage, and the losartan group (n=8) was given 20mg/kg per day losartan p.o. We compared endometriotic implant size, extent and severity of adhesion, as well as plasma and peritoneal lavage fluid cytokine levels including vascular endothelial growth factor (VEGF) and tumor necrosis factor (TNF)-, plasma inflammatory factor pentraxin-3 (PTX-3) and C-reactive protein (CRP) between the treatment groups. ResultsMean surface endometriotic area, histological score of implants, adhesion formation, plasma VEGF, TNF, PTX-3 and CRP levels were significantly lower in the losartan group compared with control (P<0.05). Furthermore, the peritoneal VEGF level was lower in the losartan group than in the control group (P<0.001), but peritoneal TNF- was similar in both groups (P>0.05). ConclusionLosartan suppressed the implant surface area of experimental endometriosis in rats and reduced the levels of plasma VEGF, TNF-, PTX-3 and CRP.Öğe Regression of experimental endometriotic implants in a rat model with the angiotensin II receptor blocker losartan(Wiley, 2015) Cakmak, Bulent; Cavusoglu, Turker; Ates, Utku; Meral, Ayfer; Nacar, Mehmet Can; Erbas, OytunAimEndometriosis is a common disease in women of reproductive age, and many different treatments have been developed, although none has provided a cure. In this study, the efficacy of losartan, an angiotensin II type 1 receptor blocker and an antiangiogenic and anti-inflammatory agent, on regression of experimental endometriotic implants in a rat model was investigated. MethodsPeritoneal endometriosis was surgically induced in 16 mature female Sprague-Dawley rats. The peritoneal endometriotic implant was confirmed after 28 days, and the animals were divided randomly into two groups. The control group (n=8) was given 4mL/day tap water by oral gavage, and the losartan group (n=8) was given 20mg/kg per day losartan p.o. We compared endometriotic implant size, extent and severity of adhesion, as well as plasma and peritoneal lavage fluid cytokine levels including vascular endothelial growth factor (VEGF) and tumor necrosis factor (TNF)-, plasma inflammatory factor pentraxin-3 (PTX-3) and C-reactive protein (CRP) between the treatment groups. ResultsMean surface endometriotic area, histological score of implants, adhesion formation, plasma VEGF, TNF, PTX-3 and CRP levels were significantly lower in the losartan group compared with control (P<0.05). Furthermore, the peritoneal VEGF level was lower in the losartan group than in the control group (P<0.001), but peritoneal TNF- was similar in both groups (P>0.05). ConclusionLosartan suppressed the implant surface area of experimental endometriosis in rats and reduced the levels of plasma VEGF, TNF-, PTX-3 and CRP.
