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Öğe Adsorption of I-131 in urine by using anion exchange resin(Springer, 2006) Unak, P.; Teksoz, S.; Medine, E. I.Öğe Extraction, radiolabeling, and biodistribution of thebaine in rats(Springer Birkhauser, 2013) Enginar, H.; Unak, P.; Lambrecht, F. Yurt; Muftuler, F. Zumrut Biber; Seyitoglu, B.; Yurt, A.; Yolcular, S.; Medine, E. I.; Bulduk, I.The goal of this study was to determine the radiopharmaceutical potential of radioiodinated thebaine. Thebaine was extracted from dry capsules of opium poppy (Papaver somniferum L.), purified using high-performance liquid chromatography, and characterized with nuclear magnetic resonance and infrared spectroscopy. The purified thebaine was labeled with I-131 using the iodogen method. Normal and receptor-blockage biodistribution studies were performed in male Albino Wistar rats. The results of the tissue distribution studies showed that the uptake of I-131-thebaine in the stomach, large intestine, spinal cord, and prostate was higher than in the other tissues. A greater uptake of radiolabeled thebaine in the rat brain was observed in the midbrain and hypothalamus. We concluded that (1) the labeling yield of I-131-thebaine was high, (2) a large amount of I-131-thebaine remained in the midbrain and hypothalamus, and (3) I-131-thebaine had enough stability for diagnostic scanning.Öğe The influence of stereoisomerism on the biological behavior of Tc-99m labeled penicillamine(Springer, 2009) Acar, C.; Teksoz, S.; Unak, P.; Muftuler, F. Z. Biber; Medine, E. I.The aim of this study is to investigate stereoisomeric behavior of penicillamine and the effect of temperature on labeling. In addition, it was explored how stereoisomerism affected biological behavior of them. In the present work, D- and L-enantiomers of penicillamine(D-PA, L-PA) were labeled with Tc-99m using SnCl2 as reducing agent and their radiopharmaceutical potentials were investigated. Quality control procedures were carried out using thin layer radiochromatography (TLRC), electrophoresis and high performance liquid radiochromatography (HPLRC). HPRLC chromatograms showed two peaks for Tc-99m-D-PA, while a single peak was observed for Tc-99m-L-PA at room temperature. However, the single peak was observed at 90 A degrees C for both isomers. Labeling yields of each isomer were found to be over 98%. Biological activity of these complexes was determined on male Albino Wistar rats by biodistribution studies. While the biodistribution result of Tc-99m-D-PA showed high uptake in the liver, maximum uptake of Tc-99m-L-PA was observed in the kidneys. Both two complexes were cleared rapidly from the blood, mainly by the renal system. Since the activity concentration of Tc-99m-D-PA at the 30th minute in the kidneys and the liver reached a maximum value and at the 120th minute, it was removed by renal and hepatobiliary excretion. As a result, it can be concluded that stereoisomerism affect not only the chemical behavior, but also differs their biological behavior of these compounds.Öğe Isolation of hydroxytyrosol from olive leaves extract, radioiodination and investigation of bioaffinity using in vivo/in vitro methods(Walter De Gruyter Gmbh, 2013) Ozkan, M.; Muftuler, F. Z. Biber; Kilcar, A. Yurt; Medine, E. I.; Unak, P.It is known that medicinal plants like olive have biological activities due to their flavonoid content such as olueropein, tyrosol, hydroxytyrosol etc. In current study, hydroxytrosol (HT) which is one of the major phenolic compounds in olive, olive leaves and olive oil, was isolated after methanol extraction and purification of olive leaves which are grown in the northern Anatolia region of Turkey. The isolated HT was radiolabeled with I-131 (I-131-HT) and the bioaffinity of this radiolabeled component of olive leaves extract was investigated by using in vivo/in vitro methods. It was found that HT could be radiolabeled with I-131 in yields of 95.6 +/- 4.4% (n = 8), and in vivo studies showed that I-131-HT is taken up by urinary bladder, stomach, small intestine, large intestine, breast and prostate. Significant incorporation of activity was observed in cell lines via in vitro studies.Öğe Multifunctional molecular imaging probes for estrogen receptors: Tc-99m labeled diethylstilbestrol (DES) conjugated, cuinp quantum dot nanoparticles (DESCIP)(Springer, 2017) Moharrami, Payam; Unak, Perihan; Guldu, Ozge Kozgus; Medine, E. I.; Gumuser, Gul; Bilgin, Elvan Sayit; Aras, OmerA theranostic nanoparticle was synthesized based on diethylstilbestrol conjugated with phosphate, copper, and indium (DESCIP) and labelled with Tc-99m which can be used for SPECT imaging of ER-enriched cancers. In vitro biological activity of Tc-99m-DESCIP was examined in breast adenocarcinoma cells (MCF-7), prostatic carcinoma cells (PC-3), and pulmonary epithelial cells (A-549). In vivo lymph node imaging was performed in normal and receptor blocked female New Zealand rabbits. Results demonstrated that Tc-99m-DESCIP and DESCIP has potential for imaging ER-enriched tumors such as breast and prostate tumors, and their metastases in the lung, as well as improving management for their therapies.Öğe Radiolabeling of codeine with I-131 and its biodistribution in rats(Springer, 2009) Enginar, H.; Unak, P.; Lambrecht, F. Y.; Muftuler, F. Z. Biber; Medine, E. I.; Yolcular, S.; Yurt, A.; Seyitoglu, B.; Bulduk, I.Codeine which was extracted from dry capsules of the opium poppy (Papaver somniferum) was purified by HPLC (High Performance Liquid Chromatography) and characterized by NMR (Nuclear Magnetic Resonance) and IR (Infrared) spectroscopy techniques. The purified compound was labeled with I-131 and biodistribution studies were performed in rats. Radioiodinated codeine distributed uniformly in the cerebellum, m.pons, striatum and hypothalamus while the other branch of brain and Stomach, urinary bladder, and small intestine uptakes were significantly higher than other tissues.Öğe SYNTHESIS OF A THERANOSTIC AGENT: RADIOIODINATED PEGYLATED PLGA-INDOCYANINE CAPSULES AND IN VITRO DETERMINATION OF THEIR BIOAFFINITY ON OVARIAN, CERVICAL AND BREAST CANCER CELLS(Lippincott Williams & Wilkins, 2017) Akman, L.; Muftuler, F. Z. Biber; Bilgi, A.; Kilcar, A. Yurt; Gokulu, S. G.; Medine, E. I.; Terek, M. C.Öğe SYNTHESIS OF A THERANOSTIC AGENT: RADIOIODINATED PEGYLATED PLGA-INDOCYANINE CAPSULES AND IN VITRO DETERMINATION OF THEIR BIOAFFINITY ON OVARIAN, CERVICAL AND BREAST CANCER CELLS(Lippincott Williams & Wilkins, 2017) Akman, L.; Muftuler, F. Z. Biber; Bilgi, A.; Kilcar, A. Yurt; Gokulu, S. G.; Medine, E. I.; Terek, M. C.Öğe Synthesis, radiolabeling and biodistribution of morphine glucuronide (mor-glu)(Springer, 2012) Enginar, H.; Unak, P.; Muftuler, F. Z. Biber; Lambrecht, F. Y.; Medine, E. I.; Yolcular, S.; Kilcar, A. Yurt; Seyitoglu, B.; Bulduk, I.The aim of this study was to synthesize a glucuronide conjugated morphine derivative which could be labeled with I-131, as a radiopharmaceutical, and to investigate its radiopharmaceutical potential using biodistribution studies in male Albino Wistar rats. Morphine was extracted from dry capsules of the opium poppy (Papaver somniferum L.). It was conjugated with UDP-glucuronic acid by using UDP-glucuronyl transferase (UDPGT) enzyme rich microsomes, purified by high performance liquid chromatography (HPLC) and characterized by nuclear magnetic resonance (NMR), infrared (IR) spectroscopy and liquid chromatography mass spectroscopy (LC-MS/MS). Normal and receptor blockage biodistribution studies were performed in male Albino Wistar rats. The results of the tissue distribution studies showed that I-131 labeled morphine glucuronide (I-131-mor-glu) uptake in the small intestine, large intestine and urinary bladder was higher than in the other tissues of the rats in the blocked receptor and unblocked receptor. A greater uptake of the radio labeled substance was observed in the hypothalamus and mid brain than in the other branches of the rats' brains.Öğe Tc-99m-exorphin-glucuronide in tumor diagnosis: Preparation and biodistribution studies in rats(Springer, 2006) Ertay, T.; Unak, P.; Ozdogan, O.; Biber, F. Z.; Zihnioglu, F.; Medine, E. I.; Durak, H.A glucuronide derivative of exorphin C called exorphin C glucuronide was obtained by enzymatic synthesis and labeled with Tc-99m using glucoheptonate (GH) as bifunctional chelating agent. Labeling efficiency was 99.7 +/- 0.03%. Biodistribution studies were performed on normal and mammary tumor bearing albino Wistar rats. In addition, scintigraphic imaging with Tc-99m-GH-exorphin C glucuronide (Tc-99m-GEG) was performed. The radiopeptide-glucuronide is rapidly cleared via the urinary system showing lower liver uptake. Results demonstrated that Tc-99m-GEG can be used as a vehicle to target tumors.Öğe Tc-99m-glucoheptonate-guanine: Synthesis, biodistribution and imaging in animals(Springer, 2008) Unak, P.; Teksoz, S.; Muftuler, F. Z. Biber; Medine, E. I.; Acar, C.; Yurekli, Y.The aim of the current study was to design a nucleotide-based radiopharmaceutical which could be labeled with Tc-99m and to investigate its radiopharmaceutical efficiency and stability. GHA (glucoheptonate) was used as bifunctional chelate. GHA was labeled with Tc-99m by SnCl2 reduction method first, and then G (guanine) was conjugated with Tc-99m-GHA at 90 C. In order to determine its radiopharmaceutical stability, thin layer radio chromatography (TLRC) and electrophoresis were employed. In addition, the results were confirmed using high performance liquid radio chromatography (HPLRC). Scintigraphic imaging was performed on rats with mammary tumors, while tissue distribution was determined on Albino Wistar rats. Labeling yield was found to be over 95% and the labeled complex maintained its stability during the study period. The lipophilicity of the Tc-99m-GHG was measured and the partition coefficient (logP) of the labeled compound calculated. The results demonstrated that the uptake of Tc-99m-GHG (Tc-99m-glucoheptonate-guanine) reached its maximum at 3 hours p.i. in stomach and intestines. Main way of excretion was renal. Hepatobiliary excretion was also observed. In conclusion, Tc-99m-GHG may be useful as a nucleotide-based radiopharmaceutical for in vivo applications.Öğe Thymidine kinase enzyme selective imaging radiopharmaceutical: Tc-99m(CO)(3)-Ganciclovir(Walter De Gruyter Gmbh, 2013) Gedik, B.; Teksoz, S.; Ichedef, C.; Kilcar, A. Y.; Medine, E. I.; Ucar, E.The aim of this study is to radiolabel ganciclovir, known as having selective antiviral properties against thymidine kinase, with technetium tricarbonylcore (Tc-99m(CO)(3)(+)) and to investigate the biological behavior of this complex in vitro and in vivo. Commercially provided Ganciclovir (GCV) was radiolabeled with Tc-99m(CO)(3)(+). Initially, optimum radiolabeling conditions were determined by analyzing factors such as temperature, pH and time. Quality control of the radiolabeled compound was performed. The radiolabeling yield was found to be 97%. The Tc-99m(CO)(3)-GCV complex also displayed good in vitro stability during the 24 h period. In vitro cell uptake studies showed that the Tc-99m(CO)(3)-GCV complex is highly uptaken in A-549, PC-3, HeLa cell lines according to the control group Tc-99m(I)-tricarbonyl core. The knowledge gained from in vivo and in vitro studies of Tc-99m(CO)(3)-GCV could contribute to the development of a new HSV1-tk gene imaging agent.
