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    Assessment of the Awareness of Prematurity and Related Problems
    (Galenos Yayincilik, 2017) Kultursay, Nilgun; Koroglu, Ozge Altun; Kizilcan, Sirmen; Kocaogut, Elif; Yalaz, Mehmet; Akisu, Mete
    Aim: Prematurity is an important health problem affecting all segments of society. In this study, we aimed to evaluate the level of awareness of prematurity and related problems. Materials and Methods: A questionnaire consisting of fifteen questions was administered to medical faculty students, pediatric residents, and to laypersons by face to face interviews. The questionnaire applied in the scope of the study had 8 open-ended questions and 7 "yes-no" questions. Results: Of the 150 people who answered the questionnaire, 92 were health workers and the remaining 54 were people from the public. The groups with and without health workers had a similar rate of awareness of the World Prematurity Awareness Day (58.7% vs. 58.6% respectively, p=0.30). All of the health care workers defined prematurity correctly while 89.7% of the laypersons gave the correct definition (p< 0.05). People from the public expected the survival of babies with higher birth weight and gestational age and this group was less informed about the short and long-term problems related to prematurity. In both groups, the awareness of physicians specializing in newborn care was low (17.4% vs. 13.8%, p=0.13). The level of awareness of the shortage of specialized physicians and nurses in neonatal care was significantly lower among laypersons than that of health care workers (p values < 0.05). Conclusion: Although our study population knew what prematurity was, they lacked knowledge about the complications of prematurity, and the quality and adequacy of the health care teams involved in the care of premature babies.
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    Genotypic discrimination of Aspergillus fumigatus strain from related species within section fumigati
    (Inst Agricultural Medicine, 2016) Giray, Betul; Kocaogut, Elif; Ucar, Fusun B.; Haliki-Uztan, Alev
    Introduction and objective. The aim was to make an exact diagnosis of 20 strains using molecular biological methods which were isolated from the atmosphere of the inpatient rooms in the Oncology and other departments of the Ege University Medical Faculty Hospital, and identified as Aspergillus fumigatus through phenotypic tests, and to determine their antibiotic susceptibility patterns. Materials and method. It was confirmed that the 20 phenotypically-identified A. fumigatus strains belonged to the section Fumigati after they were tested by the ITS-PCR method. Their sequence analysis was performed and the results sent to the NCBI GenBank, and their accession numbers were obtained. For their exact diagnosis at the species level, the beta-tub (beta-tubulin) and rodA (RodletA) genes were examined with the multiplex PCR. Anti-fungal susceptibility of the 20 strains was determined according to the M38-A2 standards. Results. As a result of ITS-PCR, it was confirmed that 19 of the 20 strains identified as A. fumigatus through the phenotypic methods belonged to the section Fumigati. However, after the detection of beta-tub and rodA genes, all 20 strains were identified as A. fumigatus. Conclusion. Although the results of the phenotypic and molecular biological tests applied to filamentous fungi do not often overlap, in this study, the results obtained from the molecular analysis confirmed the results of the phenotypic tests. However, 1 of the 20 strains phenotypically-identified as A. fumigatus was identified as Penicillium spp. as a result of ITS-PCR and sequence analysis. On the other hand, the profile obtained from beta-tub and rodA tests indicated that the strain was A. fumigatus. Based on these results, this strain is thought to belong to the Aspergilloides genus which has the features of both genera.